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FLAG-Matr3 delRRM1

Plasmid

#32881

Depositor

Yossi Shiloh

Article

Salton et al PLoS One. 2011;6(8):e23882. Epub 2011 Au

Insert

MATR3 Matrin3 (MATR3 Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

deleted amino acids 399-468

Promoter

CMV

Available Since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only
FLAG-Matr3 delZnF1

Plasmid

#32883

Depositor

Yossi Shiloh

Article

Salton et al PLoS One. 2011;6(8):e23882. Epub 2011 Au

Insert

MATR3 Matrin3 (MATR3 Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

deleted amino acids 283-322

Promoter

CMV

Available Since

Jan. 6, 2012

Availability

Academic Institutions and Nonprofits only
FLAG-Matr3 delZnF2

Plasmid

#32884

Depositor

Yossi Shiloh

Article

Salton et al PLoS One. 2011;6(8):e23882. Epub 2011 Au

Insert

MATR3 Matrin3 (MATR3 Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

deleted amino acids 793-833

Promoter

CMV

Available Since

Jan. 6, 2012

Availability

Academic Institutions and Nonprofits only
pTMN140 - UCOE-SFFV-VPH-XTEN-dCas9-IRES2-BFP-T2A-Blast

Plasmid

#238166

Purpose

Express VPH-dCas9 and BFP-T2A-BlasticidinR in mammalian cells. Lentiviral

Depositor

Thomas Norman

Article

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

VPH-dCas9

Use

CRISPR and Lentiviral

Tags

mTagBFP2

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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Pveg

Plasmid

#55173

Purpose

Very strong constitutive promoter

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Insert

veg promoter

Use

Synthetic Biology; Bacillus biobrick box

Promoter

veg

Available Since

July 7, 2014

Availability

Academic Institutions and Nonprofits only

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pET32a-hTGFa

Plasmid

#199236

Purpose

Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

Depositor

Nilson Zanchin

Article

Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

Insert

Transforming growth factor alpha, partial (TGFA Human)

Tags

TrxA-6xHis-S-tag

Expression

Bacterial

Promoter

T7

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-MITF-D

Plasmid

#38133

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

Insert

MITF-D (MITF Human)

Tags

EGFP

Expression

Mammalian

Mutation

deleted stop codon

Promoter

CMV

Available Since

Aug. 1, 2012

Availability

Academic Institutions and Nonprofits only

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sortase A pentamutant (eSrtA) in pET29

Plasmid

#75144

Purpose

evolved sortase A (eSrtA) pentamutant with improved kinetics and activity

Depositor

David Liu

Article

Chen et al Proc Natl Acad Sci U S A. 2011 Jul 12;10

Insert

sortase A pentamutant

Tags

6x His

Expression

Bacterial

Mutation

P94R, D160N, D165A, K190E, K196T. Deleted amino a…

Promoter

T7

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-HP1beta

Plasmid

#17651

Depositor

Tom Misteli

Article

Cheutin et al Science. 2003 Jan 31. 299(5607):721-5.

Insert

HP1 beta (CBX1 Human)

Tags

GFP and His

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-MITF-A

Plasmid

#38132

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

Insert

MITF-A (MITF Human)

Tags

EGFP

Expression

Mammalian

Mutation

deleted stop codon

Promoter

CMV

Available Since

July 31, 2012

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-MITF-M

Plasmid

#38131

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

Insert

MITF-M (MITF Human)

Tags

EGFP

Expression

Mammalian

Mutation

deleted stop codon

Promoter

CMV

Available Since

July 17, 2012

Availability

Academic Institutions and Nonprofits only

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pYFP-RPB1aAmr

Plasmid

#75284

Purpose

Expresses alpha-amanitin resistant EYFP tagged POLR2A in mammalian cells

Depositor

Xavier Darzacq, Robert Singer

Article

Darzacq et al Nat Struct Mol Biol. 2007 Sep;14(9):796-

Insert

POLR2A (POLR2A Human)

Tags

EYFP

Expression

Mammalian

Mutation

N792D alpha-amanitin–resistant

Promoter

CMV

Available Since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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[#GS5-RV] MitoTRACER-Donor

Plasmid

#233505

Purpose

Retroviral construct of the MitoTRACER genetic reporter to be expressed in the donor cells

Depositor

Simon Grelet

Article

Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

Insert

MitoTRACER-Donor

Use

Retroviral and Synthetic Biology

Tags

GFP11 and HA Tag

Expression

Mammalian

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-GST-Tev

Plasmid

#177142

Purpose

Bacterial vector for expression of an N-terminal GST fusion of the Tev protease

Depositor

Robert Sobol

Article

Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

Insert

Tev

Tags

GST

Expression

Bacterial

Promoter

Tac

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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JD171

Plasmid

#229483

Purpose

Adenoviral helper plasmid containing E2A, VA RNA I and L4-22k/33k genes

Depositor

Nerea Zabaleta

Article

Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

Insert

E2A, VA RNA I, L4-22k/33k

Use

AAV and Adenoviral

Expression

Mammalian

Promoter

CMV (E2A), Ef1a (L4-22k/33k) and wt promoter (VA …

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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PB-TRE-dCas9-KRAB-MeCP2

Plasmid

#122267

Purpose

SP-dCas9-KRAB-MeCP2 with doxycycline-inducible expression

Depositor

Andrea Califano

Article

Tan et al bioRxiv 2021.06.28.449297

Insert

dCas9-KRAB-MeCP2

Tags

dCas9-KRAB-MeCP2

Expression

Mammalian

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(+36)

Plasmid

#199165

Purpose

used to express a supercharged GFP variant in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(+36)

Tags

MGHHHHHHGGA

Mutation

E16R, T19R, V21K, D29K, E42K, E44K, N49E, D86K, Q…

Promoter

T7

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pDualEX-SMN2

Plasmid

#214431

Purpose

Dual-color reporter express eGFP constitutively; tdTomato expression is contingent on the exclusion of the frame-shifting cassette exon 7

Depositor

Chaolin Zhang

Article

Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

Insert

SMN2 EXON 7 (SMN2 Human)

Expression

Mammalian

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pBetaActin-Halo-CAMSAP3

Plasmid

#191329

Purpose

Expresses the fluorescently labeled microtubule minus end marker CAMSAP3 to measure microtubule retrograde flow

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

Halo-CAMSAP3 (Camsap3 Mouse)

Expression

Mammalian

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pDualIN-SMN2

Plasmid

#214430

Purpose

Dual-color reporter express eGFP constitutively; tdTomato expression is contingent on the inclusion of the frame-shifting cassette exon 7

Depositor

Chaolin Zhang

Article

Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

Insert

SMN2 EXON 7 (SMN2 Human)

Expression

Mammalian

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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