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pCDF1-S400F_RASA2-EF1-Puro

Plasmid

#114098

Purpose

Express mutant RASA2 in mamalian cells

Depositor

Yardena Samuels

Article

Arafeh et al Nat Genet. 2015 Dec;47(12):1408-10. doi:

Insert

RASA2 (RASA2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

S400F

Promoter

CMV

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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Desmoplakin II no force control (FL-based)

Plasmid

#118724

Purpose

The no force control for the FL-based human desmoplakin II tension sensor serves to detect changes in FRET between YPet(short) and mCherry that are tension-independent.

Depositor

Carsten Grashoff

Article

Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

Insert

human Desmoplakin II (1-1353)-[YPet(short)-FL-mCherry] (DSP Human)

Use

Retroviral

Tags

YPet(short)-FL-mCherry

Expression

Mammalian

Mutation

truncation after aa1353

Promoter

CMV

Available Since

Dec. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_hE2F7mg_2ntmut

Plasmid

#73074

Purpose

human E2F7 minigene (exons 11,12,13/introns cassette) with mutated snord27 binding site

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

E2F7 (E2F7 Human)

Expression

Mammalian

Mutation

partial gene, exons 11,12,13 spaced by introns, 2…

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only
pSLIK-Venus-TmiR-G12

Plasmid

#25740

Purpose

Lentiviral vector with Tet-based inducible expression of mouse G alpha 12 miR30-based shRNA, constitutive Venus fluorescent protein coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

G alpha 12 miR-shRNA (Gna12 Mouse)

Use

Lentiviral and RNAi

Tags

Venus

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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FUW TetO NET1A-V5 S46A

Plasmid

#69829

Purpose

Expresses NET1A-V5 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Neuroepithelial cell-transforming gene 1 protein A (NET1 Human)

Use

Lentiviral

Tags

v5

Expression

Mammalian

Mutation

S46A

Promoter

minimal CMV promoter with tetracycline operator

Available Since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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pDONOR-SNCAe3-WT

Plasmid

#85847

Purpose

Donor plasmid for SNCA exon3 wild type sequence. lso contains TagBFP and dTomato

Depositor

Jens Schwamborn

Article

Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

Insert

SNCA exon 3 homology arms (SNCA Human)

Expression

Bacterial and Mammalian

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pZS1[FruR-T,TreR-T]

Plasmid

#60772

Purpose

Contains PIq driving expression FruR-T, and PIq driving expression of TreR-T.

Depositor

Matthew Bennett

Article

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

FruR-T
TreR-T

Use

Synthetic Biology

Expression

Bacterial

Mutation

LacI/GalR repressor chimera. TAN DBD with FruR LB…

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-NSF/S647A

Plasmid

#74938

Purpose

Mammalian expression of human NSF mutant S647A (mutation in the D2 domain of the protein)

Depositor

Elisa Greggio

Article

Belluzzi et al Mol Neurodegener. 2016 Jan 13;11(1):1. d

Insert

NSF (NSF Human)

Tags

FLAG

Expression

Mammalian

Mutation

S647A (mutation in the D2 domain of the protein)

Promoter

CMV

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pCMV-FLAG-CHD5-WT-331-672aa

Plasmid

#68870

Purpose

Mammalian expression of CHD5 histone binding domain (331-672aa)

Depositor

Adrian Bracken

Article

Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

Insert

CHD5 (CHD5 Human)

Tags

FLAG

Expression

Mammalian

Mutation

331-672aa

Promoter

CMV

Available Since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only
Tapasin-TM

Plasmid

#254045

Purpose

psffv-tapasin-TM-FLAG

Depositor

Nikolaos Sgourakis

Article

Hwang et al Sci Adv. 2026 Jan 2;12(1):eaeb0821. doi:

Insert

Tapasin-TM (TAPBP Human)

Use

Lentiviral

Tags

FLAG Tag

Expression

Mammalian

Mutation

removed C terminal domain, replaced with that of …

Promoter

sffv

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pscAAV-U6-gThy1-CBh-mCherry-SV40late

Plasmid

#249126

Purpose

expression of gThy1 and mCherry

Depositor

Matthew Krummel

Article

Kuhn et al J Exp Med. 2026 May 4;223(5):e20251228.

Insert

gThy1 (Thy1 Mouse)

Use

AAV

Promoter

hU6

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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ePB-Puro_TT-hYAP-5SA-P2A-tdTomato-CAAX

Plasmid

#252251

Purpose

A PiggyBac donor vector for Dox-inducible expression of YAP-5SA and membrane-localized tdTomato.

Depositor

Jun Wu

Article

Oura et al Cell Stem Cell. 2025 Oct 2;32(10):1509-1

Insert

YAP-5SA (YAP1 Human)

Use

Piggybac transposon donor vector

Tags

P2A-tdTomato-CAAX

Expression

Mammalian

Promoter

tight TRE

Available Since

March 2, 2026

Availability

Academic Institutions and Nonprofits only

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pIND21-puro_SOX10-3xFLAG

Plasmid

#250322

Purpose

Tetracycline-inducible expression of SOX10. rtTA (Tet-ON) is driven by the EF1alpha promoter.

Depositor

Michael Wehr

Article

Raabe et al Cells. 2022 Jan 11;11(2):241. doi: 10.33

Insert

SOX10 (SOX10 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

TRE2

Available Since

Feb. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pJTB115

Plasmid

#248909

Purpose

Expresses yeast Smk1 and Ssp2 fused to glutathione-S-transferase in bacteria

Depositor

Edward Winter

Article

Tio et al Mol Cell Biol. 2017 May 2;37(10):e00607-

Inserts

SMK1 Mitogen-activated Protein Kinase (SMK1 Budding Yeast, Sequence is from the SK1 isolate of S. cerevisiae and will differ by 1 non-synonomous/several synonymous changes)
Ssp2 (SSP2 Budding Yeast, Sequence is from the SK1 isolate of S. cerevisiae and may differ by synonymous changes from S288C form)

Tags

glutathione-S-transferase

Expression

Bacterial

Mutation

NdeI and NcoI sites were removed for cloning purp…

Promoter

T7

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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pCBS-6756

Plasmid

#249328

Purpose

Expresses EPEC DarT2(G49D)-nSpCas9(D10A) and sg#2 targeting PTEN gene in mammalian cells

Depositor

Chase Beisel

Article

Gupta et al Nat Biotechnol. 2025 Sep 4. doi: 10.1038

Insert

EPEC DarT2(G49D)-nSpCas9(D10A)

Tags

3X Flag tag

Expression

Mammalian

Mutation

DarT2(G49D) and nSpCas9(D10A)

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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pX461-ABE8e-nSpCas9-Intergenic sgRNA

Plasmid

#237463

Purpose

All-in-one base editor expressing adenine base editor (ABE8e-nSpCas9) and gRNA control targeting Intergenic site

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

Insert

Intergenic sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

chicken beta-actin promoter & U6

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pX461-evoCDA1-nSpCas9-Intergenic sgRNA

Plasmid

#237464

Purpose

All-in-one base editor expressing cytosine base editor (evoCDA1-nSpCas9) and sgRNA control targeting Intergenic site

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

Insert

Intergenic sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

chicken beta-actin promoter & U6

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pX461-ABE8e-nSpCas9-NRF1-ss-sgRNA

Plasmid

#237466

Purpose

All-in-one adenine base editor (ABE8e-nSpCas9) with gRNA targeting NRF1-Ex7 splice site

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

Insert

gRNA targeting NRF1-Ex7 splice site

Use

CRISPR

Expression

Mammalian

Promoter

chicken beta-actin promoter & U6

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pX461-evoCDA1-nSpCas9-NRF1-ss-sgRNA

Plasmid

#237467

Purpose

All-in-one cytosine base editor (evoCDA1-nSpCas9) with sgRNA targeting NRF1-Ex7 splice site

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

Insert

sgRNA targeting NRF1-Ex7 splice site

Use

CRISPR

Expression

Mammalian

Promoter

chicken beta-actin promoter & U6

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pX462-ABE8e-nSaCas9-NRF1-ss-sgRNA

Plasmid

#237468

Purpose

All-in-one adenine base editor (ABE8e-nSaCas9) with gRNA targeting NRF1-Ex7 splice site

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

Insert

gRNA targeting NRF1-Ex7 splice site

Use

CRISPR

Expression

Mammalian

Promoter

chicken beta-actin promoter & U6

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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