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pFA6a-FKBP-GFP-HIS3MX6

Plasmid

#183023

Purpose

PCR-based tagging of yeast

Depositor

Santiago Di Pietro

Article

Lamb et al STAR Protoc. 2022 Apr 14;3(2):101323. do

Insert

FKBP

Use

Yeast genomic targeting

Available Since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

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pSP64-Lifeact-GFP

Plasmid

#135601

Purpose

Expression of Lifeact-GFP mRNA for microinjection for visualization of actin in S. purpuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

Lifeact-GFP

Use

In vitro transcription (mrna synthesis)

Tags

EGFP

Promoter

Unknown

Available Since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pSpal2At

Plasmid

#78286

Purpose

Expresses A. thaliana PAL2 in E. coli

Depositor

David Nielsen

Article

McKenna et al Metab Eng. 2011 Sep;13(5):544-54. doi: 1

Insert

PAL2 (PAL2 Mustard Weed)

Expression

Bacterial

Mutation

*see below

Promoter

lac

Available Since

June 1, 2016

Availability

Academic Institutions and Nonprofits only

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p3xEGFP-N1

Plasmid

#122163

Purpose

Expression of 3xEGFP

Depositor

Georg Mayr

Article

Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

Insert

3xEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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p6xEGFP-N1

Plasmid

#122166

Purpose

Expression of 6xEGFP

Depositor

Georg Mayr

Article

Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

Insert

6xEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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p2xEGFP-N1

Plasmid

#122162

Purpose

Expression of 2xEGFP

Depositor

Georg Mayr

Article

Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

Insert

2xEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pAllet

Plasmid

#202560

Purpose

E. coli cloning vector with large MCS (50+ unique restriction sites)

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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p18S.7 tag

Plasmid

#51730

Purpose

transcribes truncated transcript containing 18S and hybridization tag

Depositor

Vincent Mauro

Article

Burman et al Nucleic Acids Res. 2012 Sep;40(16):8085-

Insert

18S

Promoter

pol-I

Available Since

July 2, 2014

Availability

Academic Institutions and Nonprofits only

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mCherryS131C

Plasmid

#48732

Purpose

Expresses mCherryS131C in E. coli cells

Depositor

Bradley Olsen

Article

Thomas et al ACS Nano. 2011 Jul 26;5(7):5697-707. doi

Insert

mCherryS131C

Tags

6xHis

Expression

Bacterial

Mutation

changed serine 131 to cysteine

Promoter

T5

Available Since

Oct. 9, 2013

Availability

Academic Institutions and Nonprofits only

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p8xEGFP-N1

Plasmid

#122168

Purpose

Expression of 8xEGFP

Depositor

Georg Mayr

Article

Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

Insert

8xEGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

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pPI896

Plasmid

#207453

Purpose

REMI integrated Expression for mNeonGreen-Nap1

Depositor

Rob Kay

Article

Lutton et al Curr Biol. 2023 Aug 7;33(15):3083-3096.e

Insert

Nap1

Use

Unspecified

Tags

mNeonGreen

Available Since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

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p12xEGFP-N1

Plasmid

#122172

Purpose

Expression of 12xEGFP

Depositor

Georg Mayr

Article

Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

Insert

12xEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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p18S.1 G693A

Plasmid

#51728

Purpose

transcribes pactamycin resistant 18S

Depositor

Vincent Mauro

Article

Burman et al Nucleic Acids Res. 2012 Sep;40(16):8085-

Insert

18S

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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pCAH01SpR

Plasmid

#128159

Purpose

pCAH01 derivative (Ptet inducible promoter) with ahp (kanamycin resistance) CDS replaced with the aadA (streptomycin/spectinomycin resistance) CDS

Depositor

Calvin Henard

Article

Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

Type

Empty backbone

Expression

Bacterial

Promoter

Ptet

Available Since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2-H2B-mCherry

Plasmid

#135604

Purpose

Expression of H2B-mCherry mRNA for microinjection to visualize Histone 2B protein dynamics in S. purpuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

H2B-mCherry

Use

In vitro transcription (mrna synthesis)

Tags

mCherry

Promoter

Unknown

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pCT-L7.5.1

Plasmid

#42900

Depositor

Dane Wittrup

Article

Hackel et al J Mol Biol. 2008 Sep 19;381(5):1238-52.

Insert

L7.5.1

Tags

HA and c-myc

Expression

Yeast

Promoter

Gal1-10

Available Since

June 26, 2013

Availability

Academic Institutions and Nonprofits only

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pSP64-membrane-mCherry

Plasmid

#135598

Purpose

Expression of membrane-mCherry mRNA for microinjection for visualization of membrane in S. pupuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

membrane-mCherry

Use

In vitro transcription (mrna synthesis)

Tags

mCherry

Promoter

Unknown

Available Since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

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myc ATG18

Plasmid

#42530

Depositor

James Hurley

Article

Baskaran et al Mol Cell. 2012 Aug 10;47(3):339-48. doi:

Insert

ATG18 (ATG18 Budding Yeast)

Tags

myc

Expression

Yeast

Mutation

none

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
p10xEGFP-N1

Plasmid

#122170

Purpose

Expression of 10xEGFP

Depositor

Georg Mayr

Article

Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

Insert

10xEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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p5xEGFP-N1

Plasmid

#122165

Purpose

Expression of 5xEGFP

Depositor

Georg Mayr

Article

Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

Insert

5xEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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