We narrowed to 4,284 results for: TIL
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pAWPSG-Po-nCas9-BE
Plasmid#195740PurposeExpress APOVEC1-nCas9(D10A)-UGI using phenol inducible system in Methanotroph or E. coliDepositorArticleInsertDi-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterPo (phoenol-inducible promoter)Available sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
aCat TS WT (1-697 mTFP1-TSmod-Venus 698-907)
Plasmid#101299PurposeFRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.DepositorInsertalpha-Catenin
UseTagsExpressionMammalianMutationContains amino acids 1-698 and 698-906 of alpha-C…PromoterAvailable sinceJune 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-DIO-Ef1a-TVA-2A-N2cG
Plasmid#172360PurposeTargeting envA-pseodotyped RVdG vectors for specific retrograde labeling specifically from cre-expressing neuronsDepositorInsertTVA + B19-N2cG
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-EGFP-NLS
Plasmid#86677PurposeExpresses nuclear-localized GFP after lentiviral transduction. Can be used to monitor GFP leakage into the cytosol following nuclear envelope rupture events.DepositorInsertEGFP-NLS
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
ankG-mCherry
Plasmid#42566DepositorInsertankyrin G (Ank3 Rat)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTS2303-Tier1-CoTC-PPGK-NLuc_TB
Plasmid#169548PurposeTier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' CoTC insulator sequence (CoTC-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).DepositorInsertCoTC-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
UseTagsExpressionMammalianMutationPromoterPPGKAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCSD_2xNLS-SpCas9WT-NLS-3xHA-NLS-SaCas9WT-2xNLS
Plasmid#107320PurposeExpresses SpCas9 fused to SaCas9 in mammalian cellsDepositorInsertSpCas9-SaCas9 fusion
UseCRISPRTagsNLSExpressionMammalianMutationSpCas9 is fused to SaCas9PromoterCMV IE94Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DIO-Ef1a-TVA-2A-oG
Plasmid#172359PurposeTargeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling specifically from cre-expressing neuronsDepositorInsertTVA + B19-oG
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
NO- DSD Sensor 2
Plasmid#199786PurposeSynthetic gene circuitDepositorInsertNO- DSD Sensor
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-gp78c/2767
Plasmid#11429DepositorInsertgp78c (AMFR Human)
UseTagsGSTExpressionBacterialMutationn/aPromoterAvailable sinceJuly 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
aCat TL1 (1-907 mTFP1-TSmod-Venus STOP)
Plasmid#101297PurposeFRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.DepositorInsertalpha-Catenin
UseTagsExpressionMammalianMutationContains amino acids 1-906 of alpha-CateninPromoterAvailable sinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
TS MS-Sensor
Plasmid#199788PurposeSynthetic gene circuitDepositorInsertTS MS-Sensor
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHM024Env
Plasmid#137691PurposeExpress HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)DepositorInsertHIV-1 Envelope Glycoprotein
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
TT MS-Sensor
Plasmid#199789PurposeSynthetic gene circuitDepositorInsertTT MS-Sensor
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-mCherry
Plasmid#109421PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mCherry. VHH-mCherry also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, mCherry, HA, BAP and His6 epitope
UseTagsBAP, HA, His6, and T7ExpressionBacterialMutationPromoterT7Available sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Stk11)-PGKpuroBFP-W
Plasmid#105043PurposeLentiviral gRNA plasmid targeting mouse Stk11 , co-expression of TagBFPDepositorInsertStk11 (Stk11 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P41nmt1
Plasmid#39281DepositorInsertnmt1 promoter (nmt1 Fission Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutation-1163 to +6 of nmt1 locus with a 4bp deletion (TA…PromoterAvailable sinceSept. 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P81nmt1
Plasmid#39282DepositorInsertnmt1 promoter (nmt1 Fission Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutation-1163 to +6 of nmt1 locus with a 7bp deletion (AT…PromoterAvailable sinceSept. 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-GIT1
Plasmid#15226DepositorInsertG protein coupled receptor kinase-interacting protein 1 (GIT1 Human)
UseTagsGFPExpressionMammalianMutationL425V, E476K and L583PPromoterAvailable sinceSept. 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-PPP1CB-miRFP703
Plasmid#178524PurposeExpresses PP1c-miRFP703 in mammalian cellsDepositorInsertPP1c-miRFP703 (PPP1CB Synthetic, Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDL14
Plasmid#106901PurposepDL14 is conditionally replicating plasmid and expresses gam, bet and mutSK622A genes under control of PrhaB promoter for oligo-mediated E. coli chromosome engineering in MMR independent mannerDepositorInsertsgam
bet
mutS
lacI
rhaR
rhaS
UseTagsExpressionBacterialMutationLysine 622 was replaced by AlaninePromoterAvailable sinceJune 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 HA Arf6 ActQ67L
Plasmid#10835DepositorInsertArf6 Q67L (ARF6 Human)
UseTagsHAExpressionMammalianMutationQ67L. Defective in GTP hydrolysis. Constitutive…PromoterAvailable sinceApril 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
PTPRA_deltaD1-SHC
Plasmid#139615PurposeStrepIII-HA-tagged gene expressionDepositorInsertPTPRA_deltaD1 (PTPRA Human)
UseTagsStrepIII-HAExpressionMammalianMutationD1 domain deletionPromoterCMVAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSK+ chickBMPR-1B DN
Plasmid#49529Purposecloning vector for dominant negative chick BMP receptor 1BDepositorInsertBMPR-1B DN (BMPR1B Chicken)
UseTagsExpressionBacterialMutationdominant negative mutation (K231R); also P2A, M11…PromoterT3Available sinceMay 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Cluc-RMA
Plasmid#189624PurposeExpresses Cluc-RMA under the hSyn promoter. Cluc-RMA for monitoring neuronal transduction.DepositorInsertCypridina luciferase fused to Fc
UseAAV and LuciferaseTagsCluc and IgG1 FcExpressionMammalianMutationPromoterhSynAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_KRAB-MeCP2_KanR neo
Plasmid#167901PurposePiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmidDepositorInsertdCas9-KRAB-MeCP2
UseCRISPRTagsExpressionMutationD10A, D839A, H840A, N863APromoterAvailable sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP - FLAG-APEX2- hs Lamin B1
Plasmid#139442PurposeAPEX2-Lamin B1 expression plasmidDepositorInsertLMNB1 (LMNB1 Human)
UseTags3xGGGGS linker (between APEX2 and Lamin B1), APEX…ExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pK237.U6-Chimeric-CAG-loxP-stop-loxP-hspCas9 (Supernova)
Plasmid#85041PurposeThis plasmid should be used for Cre/loxP-based Supernova-CRISPR/Cas9.DepositorInsertloxP-stop-loxP
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSP1594_2x_NLS
Plasmid#110625PurposeMammalian expression vector for Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9)DepositorInsertsSt1Cas9 LMD-9
None
UseCRISPR and Synthetic BiologyTagsSV40 NLSExpressionMammalianMutationPromoterCAGAvailable sinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P41nmt-3HA
Plasmid#39284DepositorInsertnmt1 promoter (nmt1 Fission Yeast)
UseYeast genomic targetingTags3xHA, A. gossypii translation elongation factor 1…ExpressionMutation-1163 to +6 of nmt1 locus with a 4bp deletion (TA…PromoterAvailable sinceAug. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P81nmt1-3HA
Plasmid#39285DepositorInsertnmt1 promoter (nmt1 Fission Yeast)
UseYeast genomic targetingTags3xHA, A. gossypii translation elongation factor 1…ExpressionMutation-1163 to +6 of nmt1 locus with a 7bp deletion (AT…PromoterAvailable sinceAug. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGEX-KG-Itch/2793
Plasmid#11434DepositorInsertItch (Itch Mouse)
UseTagsGSTExpressionBacterialMutationn/aPromoterAvailable sinceApril 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRT498-OtUBD(V203D)
Plasmid#190090PurposeExpresses OtUBD(V203D) mutant that is unable to bind ubiquitin with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coliDepositorInsertOtUBD(V203D)
UseTags6xHis and MBPExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
U6-CjCas9-MS2gRNA-Backbone
Plasmid#210711PurposeThis plasmid express MS2 loop containing Cj Cas9 gRNADepositorInsertMS2 loop containing Cj Cas9 gRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-His3MX6-PGAL1-GFP
Plasmid#41616DepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutationPromoterAvailable sinceDec. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSC-PreE2
Plasmid#52264PurposeBacterial expression of GST-tagged TARGET proteins fused to SUMO-E2 with PreScission protease cleavage site linkerDepositorInsertUbc9 GST fusion (UBE2I Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMuLE Lenti Dest eGFP
Plasmid#62175PurposeMuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses EGFP under the control of a PGK promoter. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseLentiviral; Mule gateway destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn_CapChR2_mScarlet
Plasmid#188039PurposeExpresses CapChR2-mScarlet in mammalian neuronsDepositorInsertCapChR2-mScarlet
UseAAVTagsmScarletExpressionMutationS43D, L112C, T139C and N238E mutations in CoChRPromoterhSynAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
POLArISact
Plasmid#164971PurposeExpresses POLArISact, an orientation probe that specifically binds to F-actin, in mammalian cellsDepositorInsertPOLArISact
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FRT-EF1a-TVA-2A-N2cG
Plasmid#172361PurposeTargeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from Flp-expressing neuronsDepositorInsertTVA + B19-N2cG
UseAAVTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
KK601: pMVP (L3-L2) ecDHFR DD + polyA
Plasmid#121790PurposepMVP L3-L2 entry plasmid, contains ecDHFR DD + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term ecDHFR degron domain (stabilized by TMP) to gene of interest.DepositorInsertecDHFR Degron Domain + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSC-IntE2
Plasmid#52265PurposeBacterial expression of GST-tagged TARGET proteins fused to SUMO-E2 with GyrA intein linkerDepositorInsertUbc9 GST fusion (UBE2I Synthetic, Human)
UseTagsGST and GyrA-inteinExpressionBacterialMutationPromoterAvailable sinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMuLE Lenti Dest iRFP
Plasmid#62176PurposeMuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses iRFP under the control of a PGK promoter. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseLentiviral; Mule gateway destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-nl.mCherry-GCaMP7s
Plasmid#172385PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInsertnuclear-localized mCherry + GCaMP7s
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
JI401: Ad5 E1A/Hexon qRT-PCR standard
Plasmid#131753PurposePlasmid standard containing regions of the Ad5 E1A and Ad5 Hexon genes to create a qRT-PCR standard curve for detection of replication competent adenovirus (RCA).DepositorInsertsAd5 E1A gene fragment
Ad5 Hexon gene fragment
UseStandard for qrt-pcrTagsExpressionMutationPromoterN/AAvailable sinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2-MT mouse Axin (Axin MTFu1)
Plasmid#21287DepositorInsertAxin (Axin1 Mouse)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2009AvailabilityAcademic Institutions and Nonprofits only -
pX330.Pten.b
Plasmid#66588PurposesgRNA for Pten deletionDepositorInsertPten deletion (Pten Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-FLEX-ExSYTE
Plasmid#216263PurposeCre-dependent expressoin of ExSYTE (P2A) EGFP with 3'UTR of Rat Arc DTE under synapsin promoterDepositorInsertExSYTE P2A EGFP - Rat DTE
UseAAVTagsExpressionMutationPromoterAvailable sinceMay 8, 2024AvailabilityAcademic Institutions and Nonprofits only
