We narrowed to 3,254 results for: actin

1. pRK5 Myc-tagged PSK (K57A)

   Plasmid

   #197150

   Purpose

   Expression of kinase-deficient MYC-tagged PSK1-alpha (K57A) / TAOK2 (K57A) in mammalian cells

   Depositor

   Jonathan Morris

   Article

   Moore et al J Biol Chem. 2000 Feb 11;275(6):4311-22.

   Insert

   TAOK2 (TAOK2 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Changed K 57 to A

   Promoter

   SV40

   Available Since

   March 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST15-Eps8L2

   Plasmid

   #196534

   Purpose

   Expression of GST-Eps8L2-FL

   Depositor

   Edgar Gomes

   Article

   Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

   Insert

   Eps8L2 (EPS8L2 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   T7 promoter; lac UV5 promoter

   Available Since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDEST15-Eps8L2_1-183

   Plasmid

   #196535

   Purpose

   Expression of GST-Eps8L2_1-183

   Depositor

   Edgar Gomes

   Article

   Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

   Insert

   Eps8L2_1-183 (EPS8L2 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   T7 promoter; lac UV5 promoter

   Available Since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDEST15-Eps8L2_181-299

   Plasmid

   #196536

   Purpose

   Expression of GST-Eps8L2_181-299

   Depositor

   Edgar Gomes

   Article

   Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

   Insert

   Eps8L2_181-299 (EPS8L2 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   T7 promoter; lac UV5 promoter

   Available Since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDEST15-Eps8L2_544-715

   Plasmid

   #196540

   Purpose

   Expression of GST-Eps8L2_544-715

   Depositor

   Edgar Gomes

   Article

   Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

   Insert

   Eps8L2_544-715 (EPS8L2 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   T7 promoter; lac UV5 promoter

   Available Since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRK5mycGW-Eps8L2_529-715

   Plasmid

   #196543

   Purpose

   Expression of myc-Eps8L2_529-715

   Depositor

   Edgar Gomes

   Article

   Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

   Insert

   Eps8L2_529-715 (EPS8L2 Human)
   

   Tags

   myc

   Expression

   Mammalian

   Available Since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pB80-hsKIF5B(1-353)GCN4-L-GFP

   Plasmid

   #193714

   Purpose

   Expression of dimeric, GFP tagged, human kinesin-1 (KIF5B aa1-353)

   Depositor

   Lukas Kapitein

   Article

   Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

   Insert

   hsKIF5B(1-353)-GCN4-L-GFP (KIF5B Synthetic, Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   hsKIF5B(aa1-353);

   Promoter

   Chicken beta-actin

   Available Since

   March 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pB80-hsKIF5B(1-353)-GCN4-L-mCherry

   Plasmid

   #193715

   Purpose

   Expression of dimeric, mCherry tagged, human kinesin-1 (KIF5B aa1-353)

   Depositor

   Lukas Kapitein

   Article

   Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

   Insert

   hsKIF5B(1-353)-GCN4-L-mCherry (KIF5B Synthetic, Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   hsKIF5B(aa1-353); mCherry: Met1 Deleted

   Promoter

   Chicken beta-actin

   Available Since

   March 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR201-Eps8L2_529-715

   Plasmid

   #196533

   Purpose

   Entry vector for Gateway with Eps8L2_529-715

   Depositor

   Edgar Gomes

   Article

   Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

   Insert

   Eps8L2_529-715 (EPS8L2 Human)
   

   Expression

   Bacterial

   Promoter

   CMV promoter; T7 promoter

   Available Since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDEST15-Eps8L2_297-370

    Plasmid

    #196537

    Purpose

    Expression of GST-Eps8L2_297-370

    Depositor

    Edgar Gomes

    Article

    Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

    Insert

    Eps8L2_297-370 (EPS8L2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    T7 promoter; lac UV5 promoter

    Available Since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDEST15-Eps8L2_367-496

    Plasmid

    #196538

    Purpose

    Expression of GST-Eps8L2_367-496

    Depositor

    Edgar Gomes

    Article

    Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

    Insert

    Eps8L2_367-496 (EPS8L2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    T7 promoter; lac UV5 promoter

    Available Since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDEST15-Eps8L2_494-546

    Plasmid

    #196539

    Purpose

    Expression of GST-Eps8L2_494-546

    Depositor

    Edgar Gomes

    Article

    Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

    Insert

    Eps8L2_494-546 (EPS8L2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    T7 promoter; lac UV5 promoter

    Available Since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRK5mycGW-Eps8L2

    Plasmid

    #196542

    Purpose

    Expression of myc-Eps8L2-FL

    Depositor

    Edgar Gomes

    Article

    Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

    Insert

    Eps8L2 (EPS8L2 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Available Since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. EGFP-SPN_ARR N52R (Shank3)

    Plasmid

    #175255

    Purpose

    SPN-ARR fragment with structure opening N52R mutation in EGFP backbone for mammalian expression

    Depositor

    Johanna Ivaska

    Article

    Salomaa et al Curr Biol. 2021 Sep 29. pii: S0960-9822(

    Insert

    SPN-ARR fragment with N52R mutation from SHANK3
    

    Expression

    Mammalian

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. human TLNRD1-4H pET151

    Plasmid

    #159386

    Purpose

    Expresses human TLNRD1 4-helix domain (residues 143-273) in bacterial cells

    Depositor

    Ben Goult

    Article

    Cowell et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

    Insert

    Talin Rod Domain Containing Protein 1 (TLNRD1 Human)
    

    Tags

    his-tag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    TLNRD1 4-helix domain (residues 143-273)

    Promoter

    T7

    Available Since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pU6-chiRNA_dCrkgRNA1

    Plasmid

    #131139

    Purpose

    Encodes gRNA targeting Drosophila crk locus. Cuts 110bp upstream of the transcription start site. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).

    Depositor

    Mark Peifer

    Article

    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

    Insert

    dCrk gRNA1 (Crk Fly)
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pU6-chiRNA_dCrkgRNA2

    Plasmid

    #131140

    Purpose

    Encodes gRNA targeting Drosophila crk locus. Cuts 99bp beyond the 3' UTR. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).

    Depositor

    Mark Peifer

    Article

    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

    Insert

    dCrk gRNA1 (Crk Fly)
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJC20cdc8.27

    Plasmid

    #99362

    Purpose

    Bacterial expression vector containing cDNA encoding for the temperature sensitive fission yeast tropomyosin mutant, Cdc8.27.

    Depositor

    Dan Mulvihill

    Article

    Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

    Insert

    cdc8 (cdc8 Fission Yeast)
    

    Expression

    Bacterial

    Mutation

    Glutamic acid 129 to Lysine

    Promoter

    T7

    Available Since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENTR CMVie-Lifeact-EGFP L1-R5

    Plasmid

    #84391

    Purpose

    Multisite Gateway entry vector for expression of EGFP-tagged Lifeact in cells including neurons

    Depositor

    Ghassan Mouneimne

    Article

    Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

    Insert

    Lifeact
    

    Use

    Gateway

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV immediate early

    Available Since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-H-Ras_V12-E38

    Plasmid

    #39507

    Depositor

    Julian Downward

    Article

    Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

    Insert

    HRAS V12 E38 (HRAS Human)
    

    Expression

    Mammalian

    Mutation

    G12V and D38E

    Promoter

    CMV

    Available Since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only