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p029 End-1::EnR

Plasmid

#11029

Depositor

Ramesh Shivdasani

Article

Shoichet et al Proc Natl Acad Sci U S A. 2000 Apr 11. 9

Insert

End1::EnR (end-1, en END-1 is from C. Elegans, EnR is from Drophila)

Use

Xenopus, mammalian, avian, and zebrafish

Mutation

Dominant inhibitory GATA-binding protein End1::En…

Available Since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only

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Lenti-RNF2-ngRNA+41_EF1a-puroR

Plasmid

#207358

Purpose

Lentiviral transfer plasmid encoding hU6-driven expression of a RNF2 nicking guide RNA and EF1a-driven puromycin resistance gene.

Depositor

Marianne Carlon

Article

Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

Insert

RNF2 +41 ngRNA

Use

Lentiviral

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pHW539 (15xUAS::kin-2a(G310D)::SL2::GFP::let-858 3'UTR)

Plasmid

#107137

Purpose

Dominant negative PKA cGAL effector for C. elegans

Depositor

Paul Sternberg

Article

Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

Insert

kin-2a(G310D)::SL2::GFP

Expression

Worm

Promoter

15xUAS-delta-pes-10(basal promoter)

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR-attL5-CRY2-mCh-attL2

Plasmid

#160438

Purpose

Entry vector for cloning Cryptochrome2-mCherry using 2-fragment gateway recombination

Depositor

Nicholas Tolwinski

Article

Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

Insert

Cryptochrome 2 (CRY2 Mustard Weed)

Tags

mCherry

Expression

Bacterial

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pUCIDT-attL1-Human ABeta-attR5

Plasmid

#160436

Purpose

Entry vector for cloning human Amyloid Beta(1-42) using 2-fragment gateway recombination

Depositor

Nicholas Tolwinski

Article

Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

Insert

Homo sapiens amyloid beta precursor protein (APP Human)

Expression

Bacterial

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pMLS280

Plasmid

#73738

Purpose

Simple cloning vector

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Type

Empty backbone

Use

Unspecified

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pRL1075

Plasmid

#70688

Purpose

Use of a vector with a conditionally lethal gene, sacB, to help transfer transposon mutations to a wild type strain

Depositor

Peter Wolk

Article

Black et al Mol Microbiol. 1993 Jul;9(1):77-84.

Type

Empty backbone

Available Since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

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cEF1a-GFP

Plasmid

#135671

Purpose

Introduce constitutive (EF1a promoter) GFP cDNA into (ES) cells by recombination-mediated cassette exchange

Depositor

Florian Karreth

Insert

GFP

Use

Mouse Targeting

Available Since

March 1, 2021

Availability

Academic Institutions and Nonprofits only

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pTG611

Plasmid

#237386

Purpose

mNeonGreen::AID^NSEC^ repair template

Depositor

Ariel Pani

Article

Gibney et al Genetics. 2026 Jan 20:iyag016. doi: 10.1

Insert

mNeonGreen::AID^NSEC^

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pTG613

Plasmid

#237388

Purpose

mStayGold::AID^NSEC^ repair template

Depositor

Ariel Pani

Article

Gibney et al Genetics. 2026 Jan 20:iyag016. doi: 10.1

Insert

mStayGold::AID^NSEC^

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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KG#707

Plasmid

#110911

Purpose

Expresses the C. elegans sup-1(e995) minigene in ventral nerve cord cholinergic motor neurons

Depositor

Kenneth Miller

Article

Harterink et al J Cell Sci. 2018 Oct 22;131(20). pii: jc

Insert

sup-1 minigene

Expression

Bacterial

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCM3.12

Plasmid

#19606

Depositor

Geraldine Seydoux

Insert

spe-11 ORF+3' in pDONR201 (spe-11 Nematode)

Expression

Worm

Available Since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

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pEY21

Plasmid

#191027

Purpose

ggr-3 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

ggr-3

Expression

Worm

Mutation

None

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pEY88

Plasmid

#191081

Purpose

ggr-1 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

ggr-1

Expression

Worm

Mutation

None

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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F40F8.11 RNAi

Plasmid

#186731

Purpose

Knockdown of F40F8.11 gene

Depositor

Seung-Jae Lee

Insert

F40F8.11 targeting RNA

Use

RNAi

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pJJS010

Plasmid

#188333

Purpose

PCR template for repair templates to tag a gene with a mKate2 and mIAA7 AID degron using homology-directed repair for C. elegans.

Depositor

Mike Boxem

Article

Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

Insert

Linker::mKate2::mIAA7

Expression

Bacterial

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pJJS004

Plasmid

#188327

Purpose

PCR template for repair templates to tag a gene with a mCherry and mIAA7 AID degron using homology-directed repair for C. elegans.

Depositor

Mike Boxem

Article

Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

Insert

Linker::mCherry::mIAA7

Expression

Bacterial

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCM1.122

Plasmid

#21383

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

msp-56 promoter in pDONRP4P1R

Use

Gateway Cloning

Available Since

June 19, 2009

Availability

Academic Institutions and Nonprofits only

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pCM5.54

Plasmid

#21392

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

cye-1 3'utr in pDONRP2RP3

Use

Gateway Cloning

Available Since

June 19, 2009

Availability

Academic Institutions and Nonprofits only

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pDR3.06

Plasmid

#19368

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

daz-1 ORF+3' in pDONR201

Expression

Worm

Available Since

Jan. 21, 2009

Availability

Academic Institutions and Nonprofits only

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