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pRN3P-HA-Suv4-20h2mut

Plasmid

#86692

Purpose

For transcription of of Suv4-20h2mut mRNA preceded by an HA-tag in 5'

Depositor

Maria-Elena Torres-Padilla

Article

Eid et al Genes Dev. 2016 Nov 15;30(22):2513-2526.

Insert

histone-lysine N-methyltransferase KMT5C mutant (Kmt5c Mouse)

Use

Tags

HA

Expression

Mutation

Mutated asparagine 273 to cysteine 276 (NHDC) int…

Promoter

T3

Available since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pL6-H2B-iRFP720-bla

Plasmid

#189991

Purpose

Lentivirial plasmid for nuclear iRFP-720 expression with blasticidin resistance

Depositor

Achim Kramer

Article

Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

Human histone H2B

Use

Lentiviral

Tags

iRFP720

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pVH19-Tier1-PhCMV-p300core

Plasmid

#169572

Purpose

Tier-1 vector encoding PhCMV-driven histone acetyltransferase p300 core domain expression (PhCMV-p300core-pA)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven P300core (EP300 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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Clim-DC2-H2B2-Venus

Plasmid

#129556

Purpose

pClimDC-H2B:mV nuclear marker

Depositor

Inaki Ruiz-Trillo

Article

Kozyczkowska et al Curr Biol. 2021 Sep 27;31(18):4104-4110.

Insert

Histone 2B fused to venus

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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MTK3_006

Plasmid

#123722

Purpose

Encodes human histone H2B as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

H2B

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV5-Flag-HDAC3

Plasmid

#63676

Purpose

Mammalian expression of Flag tagged HDAC3

Depositor

Lingfang Zeng, Qingbo Xu

Article

Zeng et al J Cell Biol. 2006 Sep 25;174(7):1059-69.

Insert

HDAC3 (Hdac3 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only
pGFP-HBO1-G485A pc2201

Plasmid

#167570

Purpose

The human HBO1 is fused to C-terminal GFP of EGFP-C1 (Clontech)

Depositor

Cristina Cardoso

Article

Heinz et al Epigenetics Chromatin. 2019 Mar 14;12(1)

Insert

histone acetyltransferase 1 (KAT7 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

G485A

Promoter

CMV

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCVhyg-H3.3WT-FLAG/HA

Plasmid

#128740

Purpose

Expression of histone H3.3WT-FLAG/HA

Depositor

Kai Ge

Article

Jang et al Nucleic Acids Res. 2019 Jan 25;47(2):607

Insert

H3.3 WT (H3-3A Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mutation

Promoter

MSCV

Available since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pTY-EF1a-wild type mKdm2b-Flag

Plasmid

#61739

Purpose

Express wild-type mouse histone demethylase Kdm2b

Depositor

Yi Zhang

Article

He et al Nat Cell Biol. 2013 Apr;15(4):373-84. do

Insert

Kdm2b (Kdm2b Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 6, 2015

Availability

Academic Institutions and Nonprofits only
pmScarlet-i_H2A_C1

Plasmid

#85053

Purpose

In vivo visualization of the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

H2A (H2AC16 Human)

Use

Tags

mScarlet-i

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pMTTH+mEGFP-H1.4

Plasmid

#157799

Purpose

Expression of mEGFP and human linker histone H1.4 fusion protein

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

mEGFP-H1.4 (H1-4 Human)

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

pTAC

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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dSpyCas9-p300-P2A-CASANOVA

Plasmid

#113129

Purpose

Co-expresses dSpyCas9 fused to the p300 histone acetyltransferase core domain and CASANOVA

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

dCas9-p300-P2A-CASANOVA

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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Ad-Flag-HDAC3

Plasmid

#63675

Purpose

adenoviral expression of Flag tagged HDAC3

Depositor

Lingfang Zeng, Qingbo Xu

Article

Zeng et al J Cell Biol. 2006 Sep 25;174(7):1059-69.

Insert

HDAC3 (Hdac3 Mouse)

Use

Adenoviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only
pMSCVhyg-H3.3K4M-FLAG/HA

Plasmid

#128741

Purpose

Expression of histone H3.3K4M-FLAG/HA

Depositor

Kai Ge

Article

Jang et al Nucleic Acids Res. 2019 Jan 25;47(2):607

Insert

H3.3K4M (H3-3A Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mutation

K4M

Promoter

MSCV

Available since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pmScarlet-H_H2A_C1

Plasmid

#85052

Purpose

In vivo visualization of the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

H2A (H2AC16 Human)

Use

Tags

mScarlet-H

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pMTTH+H1.4ΔCTD

Plasmid

#157797

Purpose

Expression of human linker histone H1.4 without c-terminal tail

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

H1.4ΔCTD (H1-4 Human)

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

pTAC

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pIRESN2 H2B-GFP-uvr8at(2x)

Plasmid

#44970

Depositor

Thanos Halazonetis

Article

Crefcoeur et al Nat Commun. 2013;4:1779. doi: 10.1038/nc

Insert

H2B-GFP-UVR8(2x) (UVR8, H2BC21 Human, Mustard Weed)

Use

Tags

internal GFP

Expression

Mammalian

Mutation

tandem repeat of UVR8 (both have Methionine 1 del…

Promoter

pCMV IE

Available since

June 6, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-synp-F-H2B-GCaMP6f

Plasmid

#102994

Purpose

Expresses histone fused GCaMP6f under the synapsin promoter

Depositor

David Cox

Article

Skocek et al Nat Methods. 2018 Jun;15(6):429-432. doi

Insert

H2B-GCaMP6f

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

human synapsin I promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pME-H2B

Plasmid

#108883

Purpose

Multisite gateway vector for 5' tagging with the human histone H2B sequence (for nuclear localisation)

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

H2B (human amino acid sequence but different codon usage) (H2BC21 Human, Synthetic)

Use

Multisite gateway

Tags

Expression

Mutation

human amino acid sequence but different codon usa…

Promoter

Available since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pQCXIpuro-H3.3K4M-FLAG/HA

Plasmid

#128742

Purpose

Expression of histone H3.3K4M-FLAG/HA

Depositor

Kai Ge

Article

Jang et al Nucleic Acids Res. 2019 Jan 25;47(2):607

Insert

H3.3K4M (H3-3A Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mutation

K4M

Promoter

CMV

Available since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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