We narrowed to 2,181 results for: levi

1. pAAV-ITR-CMV-mCherry-1xU6-LtR-wt(TAG)

   Plasmid

   #217366

   Purpose

   Expresses E. coli leucine tRNA and a wild-type mCherry reporter; can be packaged into AAV

   Depositor

   Abhishek Chatterjee

   Article

   Huang et al ACS Synth Biol. 2024 Jul 19;13(7):2141-2

   Insert

   E. coli leucine tRNA for TAG suppression
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFB1.HMBP.PrS.CBX8ΔChromo

   Plasmid

   #224708

   Purpose

   Expresses a human CBX8 truncation (deletion of the chromodomain) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag

   Depositor

   Chen Davidovich

   Article

   Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

   Insert

   CBX8 chromodomain truncation (CBX8 Human)
   

   Use

   Tags

   HMBP

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFBOH-MHL.mEGFP-CBX8

   Plasmid

   #224710

   Purpose

   Expresses N-terminal hexahistidine-tagged human CBX8 in insect cells, with a monomeric variant EGFP tag.

   Depositor

   Chen Davidovich

   Article

   Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

   Insert

   CBX8 (CBX8 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   Oct. 18, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pFB1.HMBP.PrS.CBX8-KR21A

   Plasmid

   #224709

   Purpose

   Expresses a human CBX8 mutant (KR21A) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

   Depositor

   Chen Davidovich

   Article

   Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

   Insert

   CBX8 KR21A mt (CBX8 Human)
   

   Use

   Tags

   HMBP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFB1.HMBP.PrS.CBX8

   Plasmid

   #224707

   Purpose

   Expresses human CBX8 in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

   Depositor

   Chen Davidovich

   Article

   Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

   Insert

   CBX8 (CBX8 Human)
   

   Use

   Tags

   HMBP

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pXPR_003.gEZH2

   Plasmid

   #220318

   Purpose

   Expresses guide RNA for CRISPR/Cas9 knockout of EZH2 for knockout/rescue experiments in mammalian cells.

   Depositor

   Chen Davidovich

   Article

   Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

   Insert

   EZH2 gRNA (EZH2 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFB1.HMBP.PrS.EZH2-mt2

   Plasmid

   #220240

   Purpose

   Expression of an EZH2 mutant in insect cells.

   Depositor

   Chen Davidovich

   Article

   Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

   Insert

   EZH2 mt 2 (EZH2 Human)
   

   Use

   Tags

   6x His, MBP

   Expression

   Insect

   Mutation

   F32A, R34A, D36A, K39A, PRKKKR494-499NAAIRS

   Promoter

   Polyhedrin

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFB1.HMBP.PrS.EZH2-mt1

   Plasmid

   #220239

   Purpose

   Expression of an EZH2 mutant in insect cells.

   Depositor

   Chen Davidovich

   Article

   Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

   Insert

   EZH2 mt 1 (EZH2 Human)
   

   Use

   Tags

   6x His, MBP

   Expression

   Insect

   Mutation

   R27A, R31A, F165A

   Promoter

   Polyhedrin

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET15b MOSPD2 [315-445]

   Plasmid

   #186474

   Purpose

   Expression of human MOSPD2 (MSP domain from residue 315 to 445), fused to a cleavable 6HIS tag, in bacteria

   Depositor

   Catherine Tomasetto

   Article

   Di Mattia et al EMBO J. 2020 Dec 1;39(23):e104369. doi:

   Insert

   MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
   

   Use

   Tags

   6HIS

   Expression

   Bacterial

   Mutation

   MSP domain [315-445]

   Promoter

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti CMV Hygro dn-cHSF1

    Plasmid

    #134732

    Purpose

    Lentiviral expression vector for constitutively active dominant-negative HSF1

    Depositor

    Matthew D. Shoulders

    Article

    Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

    Insert

    dn-cHSF1 (HSF1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 186-202, 379−529

    Promoter

    CMV

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti CMV/TO Zeo dn-cHSF1

    Plasmid

    #134734

    Purpose

    Lentiviral expression vector for inducible expression of a constitutively active dominant-negative HSF1

    Depositor

    Matthew D. Shoulders

    Article

    Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

    Insert

    dn-cHSF1 (HSF1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 186-201, 380−529

    Promoter

    CMV/TO

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDest40 DHFR.dn-cHSF1

    Plasmid

    #134735

    Purpose

    Mammalian expression vector for constitutively active dominant-negative HSF1 fused to DHFR destabilized domain

    Depositor

    Matthew D. Shoulders

    Article

    Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

    Insert

    DHFR.dn-cHSF1 (HSF1 Human)
    

    Use

    Tags

    DHFR

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 186-202, 379−529

    Promoter

    CMV

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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13. pXJ219-pAAV-PB3'IR-U6-gRNA-EF1a-mtagBFP2-KASH-PB5'IR

    Plasmid

    #210525

    Purpose

    AAV piggybac transposon vector, with mtagBFP-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.

    Depositor

    Xin Jin

    Article

    Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

    Inserts

    mtagBFP-KASH
    U6-gRNA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pXJ385-pAAV-PB3’IR-U6-gRNA-EF1a-mScarlet-KASH-PB5’IR

    Plasmid

    #224435

    Purpose

    AAV piggybac transposon vector, with mScarlet-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.

    Depositor

    Xin Jin

    Article

    Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

    Inserts

    mScarlet-KASH
    U6-gRNA
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pXJ218-pAAV-PB3'IR-U6-gRNA-directcapture-EF1a-mtagBFP2-KASH-PB5'IR

    Plasmid

    #213480

    Purpose

    AAV piggybac transposon vector, with mtagBFP-KASH reporter and gRNA cloning site. Compatible with 10X 3' and 5' gRNA capture technology.

    Depositor

    Xin Jin

    Article

    Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

    Inserts

    mtagBFP-KASH
    U6-gRNA-directcapture
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pXJ220-pAAV-PB3'IR-U6-gRNA-EF1a-GFP-KASH-PB5'IR

    Plasmid

    #213481

    Purpose

    AAV piggybac transposon vector, with GFP-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.

    Depositor

    Xin Jin

    Article

    Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

    Inserts

    GFP-KASH
    U6-gRNA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-N-Flag-NLRP3 1-90

    Plasmid

    #75137

    Purpose

    Expresses N-terminal Flag-tagged amino acids 1-90 of NLRP3 (the pyrin domain) in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    fragment encoding amino acids 1-90

    Promoter

    CMV

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA 3.2/V5-DEST CDC42EP1 S192A

    Plasmid

    #69820

    Purpose

    Expresses CDC42EP1-V5 in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    CDC42 effector protein (CDC42EP1 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    S192A

    Promoter

    CMV

    Available since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHIV-EGFP.Flag-EZH2-mt2

    Plasmid

    #220245

    Purpose

    Expresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.

    Depositor

    Chen Davidovich

    Article

    Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

    Insert

    EZH2 mt 2 (EZH2 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    F32A, R34A, D36A, K39A, PRKKKR494-499NAAIRS

    Promoter

    EF-1α

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-N-Flag-ASC1

    Plasmid

    #75134

    Purpose

    Expresses N-terminal Flag-tagged ASC1 in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    PYD and CARD domain containing (Pycard Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    F23S (please see depositor comments below)

    Promoter

    CMV

    Available since

    Aug. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge