We narrowed to 6,573 results for: tac

1. pMAL-MBP-Rac1-T17N

   Plasmid

   #214293

   Purpose

   Express dominant negative Rac1 fused with MBP

   Depositor

   Cliff Stains

   Article

   He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

   Insert

   Dominant negative Rac1 (RAC1 Human)
   

   Use

   Tags

   Maltose-binding protein (MBP)

   Expression

   Bacterial

   Mutation

   Rac1-T17N

   Promoter

   Tac

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMAL-MBP-FRB-Rac1/N12

   Plasmid

   #214294

   Purpose

   Express split-Rac1 fragment fused with FRB and MBP

   Depositor

   Cliff Stains

   Article

   He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

   Insert

   Split-Rac1 fragment fused with FRB (RAC1 Human)
   

   Use

   Tags

   Maltose-binding protein (MBP)

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX-4T1-GST-TEV-NAP1

   Plasmid

   #217610

   Purpose

   For expression in E. coli of NAP1

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv September 25, 202

   Insert

   NAP1 (AZI2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   TAC promoter

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-2T_RV-B14_VP1

   Plasmid

   #202074

   Purpose

   Bacterial expression of VP1 protein from rhinovirus B14 (RV-B14). Contains N-terminal GST-tag and C-terminal His6-tag.

   Depositor

   Vesa Hytönen

   Article

   Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

   Insert

   RV-B14 VP1 protein with Histag
   

   Use

   Tags

   6xHis-tag and GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX-2T_RV-A89_VP1

   Plasmid

   #202073

   Purpose

   Bacterial expression of VP1 protein from rhinovirus A89 (RV-A89). Contains N-terminal GST-tag and C-terminal His6-tag.

   Depositor

   Vesa Hytönen

   Article

   Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

   Insert

   RV-A89 VP1 protein with Histag
   

   Use

   Tags

   6xHis-tag and GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. GST-RAPH1-F2

   Plasmid

   #194864

   Purpose

   Express the RAPH1 fragment (535-868) tagged to GST in bacteria.

   Depositor

   Guillaume Jacquemet

   Article

   Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

   Insert

   RAPH1 1062-1250 (RAPH1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   amino acids 1062-1250

   Promoter

   Tac

   Available since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX-6P-1 Drosophila melanogaster CBS-pair domain

   Plasmid

   #166436

   Purpose

   Express pGEX-6P-1 Drosophila melanogaster CBS-pair domain

   Depositor

   Kalle Gehring

   Article

   Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

   Insert

   Drosophila melanogaster CBS-pair domain (Cbs Fly)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Tac, LacI, Amp

   Available since

   Sept. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. FKBP-EGFP-RanGAP*

   Plasmid

   #175238

   Purpose

   Bacterial expression and purification, low affinity SUMOylation substrate that can recruited to FRB with rapamycin, point mutation F562A reduces affinity for E2, increasing the Km

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   RANGAP1 (RANGAP1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Amino acid 562 F to A

   Promoter

   tac

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX-PP-NEDD4L-WW1(193-226)

   Plasmid

   #166701

   Purpose

   expresses NEDD4L WW1 in bacterial cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

   Insert

   NEDD4L WW1 domain
   

   Use

   Tags

   GST, HRV 3C site, and Thrombin site

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEX-PP-NEDD4L-WW2(385-418)

    Plasmid

    #166702

    Purpose

    expresses NEDD4L WW2 in bacterial cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

    Insert

    NEDD4L WW2 domain
    

    Use

    Tags

    GST, HRV 3C site, and Thrombin site

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGEX-PP-NEDD4L-WW3(497-530)

    Plasmid

    #166703

    Purpose

    expresses NEDD4L WW3 in bacterial cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

    Insert

    NEDD4L WW3 domain
    

    Use

    Tags

    GST, HRV 3C site, and Thrombin site

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGEX-PP-NEDD4L-WW4(548-581)

    Plasmid

    #166704

    Purpose

    expresses NEDD4L WW4 in bacterial cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

    Insert

    NEDD4L WW4 domain
    

    Use

    Tags

    GST, HRV 3C site, and Thrombin site

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. EcNGsC-MutY-pKK223

    Plasmid

    #131376

    Purpose

    Low level expression of E.coli G.stearothermophillus MutY chimera protein

    Depositor

    Martin Horvath

    Article

    Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021

    Insert

    EcNGsC MutY chimera
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Fusion of two MutY genes. Residues 1-225 are from…

    Promoter

    tac

    Available since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEX-4T-3-cytXlATL2-R232Q

    Plasmid

    #124064

    Purpose

    Control protein. The R232Q mutation abolishes dimerization activity, so this construct is inactive compared to wild type cytATL

    Depositor

    Tom Rapoport

    Article

    Wang et al J Cell Biol. 2013 Dec 9;203(5):801-14. d

    Insert

    cytXlATL2-R232Q
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFM101

    Plasmid

    #40147

    Depositor

    Geraldine Seydoux

    Article

    Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

    Insert

    gfp::par-2 wild-type RNAi resistant (par-2 Nematode)
    

    Use

    Tags

    GST and eGFP

    Expression

    Bacterial

    Mutation

    Base pairs 6-523 of par-2 were recoded to create …

    Promoter

    tac

    Available since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pFM102

    Plasmid

    #40148

    Depositor

    Geraldine Seydoux

    Article

    Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

    Insert

    gfp::par-2 RNAi resistant R183-5A (par-2 Nematode)
    

    Use

    Tags

    GST and eGFP

    Expression

    Bacterial

    Mutation

    R183-5A; base pairs 6-523 of par-2 were recoded t…

    Promoter

    tac

    Available since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pJK01_Rho_minprox_DsREd

    Plasmid

    #173489

    Purpose

    PCR template for reporter gene with mouse Rhodopsin promoter with DsRed

    Depositor

    Barak Cohen

    Article

    White et al Proc Natl Acad Sci U S A. 2013 Jul 16;11

    Insert

    Mouse Rhodopsin promoter driving DsRed reporter gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Mouse Rhodopsin

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSLQ7535 pHR (hU6-crSURF2-EFS-PuroR-WPRE)

    Plasmid

    #214879

    Purpose

    Lentiviral vector encoding RfxCas13d targeting SURF2 guide array

    Depositor

    Stanley Qi

    Article

    Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

    Insert

    hU6-crSURF2-EFS-PuroR-WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAM/CBA-eGFP-miRNegativex3-WPRE-bGHpA

    Plasmid

    #194249

    Purpose

    Expresses 3 non-targeting miRNAs

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    miRNegative
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX4T3-GST-NQO1

    Plasmid

    #177140

    Purpose

    Bacterial vector for expression of an N-terminal GST fusion of NQO1 with a TEV protease site located between the GST tag and NQO1

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    NQO1 (NQO1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge