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Plasmid#47066PurposeFX cloning E.coli expression vector with T7 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tagsDepositorTypeEmpty backboneUseTags3C protease site (PreScission site), GFP, and dec…ExpressionBacterialMutationPromoterT7Available sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only
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pDONR221-Hyg
Plasmid#48994PurposeGateway adapted transfer of Hygromycin resistance geneDepositorInserthygromycin B phosphotransferase
UseGateway attb1 & attb2TagsExpressionMutationPromoterAvailable sinceApril 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEBB-TB
Plasmid#36099DepositorTypeEmpty backboneUseTagsBT-tagExpressionMammalianMutationPromoterEF1aAvailable sinceJune 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pQE-WT-T3-R5-RRVH
Plasmid#63623PurposeT3-R5-RRVH RNAP mutantDepositorInsertT3 RRVH RNAP
UseTagsExpressionMutationT745K, N748D, L749M, M750I, G753R, H772R, E775V, …PromoterT5/LacAvailable sinceJuly 29, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBXNH3
Plasmid#47067PurposeFX cloning E.coli expression vector with araBAD promoter and N-terminal 10x His tag and 3C protease cleavage siteDepositorTypeEmpty backboneUseTags3C protease site (PreScission site) and decaHis (…ExpressionBacterialMutationPromoteraraBADAvailable sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pQE-WT-KIF-R5-IRH
Plasmid#63629PurposeK1F-R5-IRH RNAP mutantDepositorInsertKIF-R5-IRH RNAP
UseTagsExpressionMutationL749I, Q754S, R756N, I761V, H772R, and Q786HPromoterT5/LacAvailable sinceJune 1, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
RGG-NanoLuc-RGG
Plasmid#176642PurposeNanoLuc enzyme fused to LAF-1 RGG domainDepositorInsertRGG-NanoLuc-RGG
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-CTSS
Plasmid#158445PurposeGateway Cloning compatible entry vector for the human CTSS gene.DepositorInsertCTSS (CTSS Human)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pfbiRS
Plasmid#67283PurposeExpresses the fbiC gene from Mycobacterium smegmatisDepositorInsertfbiC gene from Mycobacterium smegmatis
UseTagspolyhistidineExpressionMammalianMutationnon knownPromoterAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
NK641
Plasmid#109100PurposeReporter gene expression in S. rosettaDepositorInsertPTSG_07215
UseChoanoflagellate expressionTagsExpressionMutationPromoterAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
NK601
Plasmid#109070PurposeParental vector for making S. rosetta expression vectorsDepositorInsertPTSG_03784
UseChoanoflagellate expressionTagsExpressionMutationPromoterAvailable sinceMay 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28CLY4
Plasmid#21763DepositorInsertECFP -EYFP linked by flexible linker containing 4 SGGSGG repeats
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceDec. 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
NK590
Plasmid#109084PurposeReporter gene expression in S. rosettaDepositorInsertmPapaya
UseChoanoflagellate expressionTagsExpressionMutationPromoterAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNJB80
Plasmid#51518PurposeGateway entry vector with attL5 and attL2 sites flanking a CmR and ccdB gene. In our hands, the multisite reaction still proceeds efficiently.DepositorTypeEmpty backboneUseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceMarch 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
NK594
Plasmid#109085PurposeReporter gene expression in S. rosettaDepositorInsertTagRFP-T
UseChoanoflagellate expressionTagsExpressionMutationPromoterAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF3C06
Plasmid#70979PurposeGateway entry cloneDepositorInsertCG9246
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
NK649
Plasmid#109105PurposeReporter gene expression in S. rosettaDepositorInsertPTSG_06009
UseChoanoflagellate expressionTagsExpressionMutationPromoterAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF3D11
Plasmid#71007PurposeGateway entry cloneDepositorInsertAcf1
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSKB3.TM1777
Plasmid#11421DepositorInsertNusA (nusA T. maritima)
UseTags6x His and TEV protease siteExpressionBacterialMutationPromoterAvailable sinceApril 10, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGGEV_5'_Linker
Plasmid#49291PurposeGolden Gate entry vector at position 5, small linker insert, final entry vector for the assemblyDepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterAvailable sinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only