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PCDNA3-N3FLAG-MYB-(1-327)

Plasmid

#105612

Purpose

transient express MYB N terminal fragment with 3*FLAG tag at N terminal in HEK 293T cells

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

N terminal fragment of MYB (mouse), amino acids 1-327 (Myb Mouse)

Tags

3*FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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PCDNA3-N3FLAG-MYB-DBD(1-200)

Plasmid

#105610

Purpose

transient express MYB DBD fragment with 3*FLAG tag at N terminal in HEK 293T cells

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

DBD fragment of MYB (mouse), amino acids 1-200 (Myb Mouse)

Tags

3*FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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PCDNA3-N3HA-TAF12-HFD(50-130)

Plasmid

#105613

Purpose

transient express TAF12 HFD with 3* HA tag at N terminal in HEK 293T cells

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF12 HFD amino acid 50-130 with HA tag (Taf12 Mouse)

Tags

3*HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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PCDNA3-TAF4(827-909)

Plasmid

#105614

Purpose

transient express TAF4 HFD in HEK 293T cells

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF4 HFD amino acid 827-909 (Taf4 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pNB695

Plasmid

#60144

Purpose

Disintegrator plasmid encoding MET17 promoter driven green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

GrLuc

Tags

ClpXP degron and Nuclear localization signal (NLS…

Expression

Yeast

Promoter

MET17pr

Available Since

Dec. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pNB697

Plasmid

#60146

Purpose

Disintegrator plasmid encoding MET17 promoter driven red beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

RdLuc

Tags

ClpXP degron and Nuclear localization signal (NLS…

Expression

Yeast

Promoter

MET17pr

Available Since

Dec. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pPN218

Plasmid

#91677

Purpose

Express sgRNA targeting human TLE3

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

TLE3 (TLE3 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN219

Plasmid

#91678

Purpose

Express sgRNA targeting human TLE3

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

TLE3 (TLE3 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pIS1 IMP-1 rc

Plasmid

#21643

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

IGF-II mRNA-binding protein 1 (IGF2BP1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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pLKO.1 shPIK3CD.v2 puro

Plasmid

#58707

Purpose

Lentiviral shRNA vector for knockdown of human PIK3CD

Depositor

Kevin Janes

Article

Bajikar et al Proc Natl Acad Sci U S A. 2014 Feb 4;111

Insert

shPIK3CD (PIK3CD Human)

Use

Lentiviral

Expression

Mammalian

Available Since

May 5, 2016

Availability

Academic Institutions and Nonprofits only
pMXs-B3

Plasmid

#31707

Depositor

Duanqing Pei

Article

Liao et al J Biol Chem. 2011 May 13. 286(19):17359-

Insert

miR-20b (Mir20b Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pEBB-3XMyc-TRAF2 S274A

Plasmid

#44107

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 S274A (TRAF2 Human)

Tags

3xMyc

Expression

Mammalian

Mutation

Serine 274 to Alanine

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only
pSG5-mEKLF

Plasmid

#67838

Purpose

Expression of murine EKLF (nucleotides 71-1215) driven by SV40 promoter

Depositor

James Bieker

Article

Miller et al Mol Cell Biol. 1993 May;13(5):2776-86.

Insert

EKLF (Klf1 Mouse)

Tags

none

Expression

Mammalian

Promoter

SV40

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only
mEos2-MyosinIIA-C-18

Plasmid

#57403

Purpose

Localization: Cytoskeleton, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Burnette et al J Cell Biol. 2014 Apr 14;205(1):83-96. d

Insert

MyosinIIA

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pNB788

Plasmid

#60165

Purpose

Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by RNR1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc-yEVenus

Expression

Yeast

Mutation

FLuc has A4V & S504G (no functional effect)

Promoter

RNR1pr

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pBJ1 neo-mo FceRI beta

Plasmid

#8604

Depositor

Jean Kinet

Article

Ra et al J Biol Chem 1989 Sep 15;264(26):15323-7.

Insert

mouse Fc epsilon RI beta cDNA (Ms4a2 Mouse)

Expression

Mammalian

Available Since

June 16, 2005

Availability

Academic Institutions and Nonprofits only
pEYFP-N1-hRIP3-FL G457D

Plasmid

#61367

Purpose

Expresses Human full length RIPK3 with G457Dmutation in the mammalian cells

Depositor

Francis Chan

Article

Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

Insert

Full length human RIP3 with G457D mutation (RIPK3 Human)

Tags

EYFP

Expression

Mammalian

Mutation

changed G457 to D

Available Since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only
pEYFP-N1-hRIP3-FL N464D

Plasmid

#61369

Purpose

Expresses Human full length RIPK3 with N464D mutation in the mammalian cells

Depositor

Francis Chan

Article

Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

Insert

Full length human RIP3 with N464D mutation (RIPK3 Human)

Tags

EYFP

Expression

Mammalian

Mutation

changed N464to D

Available Since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only
pRK5-HA-SH3BP4 W92A

Plasmid

#46893

Purpose

expresses HA tagged SH3BP4 W92A mutant in mammalian cells

Depositor

Do-Hyung Kim

Article

Kim et al Mol Cell. 2012 Jun 29;46(6):833-46. doi:

Insert

SH3BP4 W92A (SH3BP4 Human)

Tags

HA

Expression

Mammalian

Mutation

W92A (mutated SH3 domain)

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pMXs-B2

Plasmid

#31706

Depositor

Duanqing Pei

Article

Liao et al J Biol Chem. 2011 May 13. 286(19):17359-

Insert

miR-18b (Mir18b Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only