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MDM4_CRISPRi_gRNA_1

Plasmid

#195132

Purpose

gRNA from the Weissman CRISPRi library targeting MDM4, cloned into a third generation gRNA backbone with mCherry

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

MDM4 CRISPRi gRNA 1 (MDM4 Human)

Expression

Mammalian

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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MDM4_Deletion_Downstream_gRNA_1

Plasmid

#195136

Purpose

gRNA in a third generation gRNA backbone with mCherry, targeting region immediately downstream of MDM4, to be used with MDM4_Deletion_Upstream_gRNA_1/2 for MDM4 deletion

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

MDM4 Deletion Downstream gRNA 1 (MDM4 Human)

Expression

Mammalian

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-R/G-ICUE

Plasmid

#181849

Purpose

ICUE cAMP sensor using sfGFP and mRuby2 as the FRET donor and acceptor.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

R/G-ICUE (RAPGEF3 Human)

Tags

mRuby2 and sfGFP

Expression

Mammalian

Mutation

Glutamine 122 in Epac1 domain mutated to glutamat…

Promoter

CMV

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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R620-M07-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-541)-His6 E484K

Plasmid

#170193

Purpose

Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), E484K mutant

Depositor

Dominic Esposito

Article

Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

Insert

SARS-CoV-2 RBD (S SARS-CoV-2)

Tags

His6

Expression

Mammalian

Mutation

E484K

Promoter

CMV51

Available Since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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R620-M08-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(318-529)-3C-His8-SBP E484K

Plasmid

#170194

Purpose

Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), E484K mutant

Depositor

Dominic Esposito

Article

Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

Insert

SARS-CoV-2 RBD (S SARS-CoV-2)

Tags

3C-His8-SBP

Expression

Mammalian

Mutation

E484K

Promoter

CMV51

Available Since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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R620-M03-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-541)-His6 N501Y

Plasmid

#170189

Purpose

Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), N501Y mutant from B.1.1.7 variant

Depositor

Dominic Esposito

Article

Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

Insert

SARS-CoV-2 RBD (S SARS-CoV-2)

Tags

His6

Expression

Mammalian

Mutation

N501Y

Promoter

CMV51

Available Since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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SOD1_pcDNA6.2/EmGFP-Bsd

Plasmid

#176964

Purpose

Mammalian expression vector encoding SOD1 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

SOD1 (SOD1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-ZNF385A

Plasmid

#101615

Purpose

Donor Vector containing ZNF385A transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF385A (ZNF385A Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-FOXD4L3

Plasmid

#101491

Purpose

Donor Vector containing FOXD4L3 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

FOXD4L3 (FOXD4L3 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1delZn3-L713F

Plasmid

#173934

Purpose

Bacterial expression of PARP1 lacking Zn3 domain but containing L713F gain-of-function mutation

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delZn3-L713F (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 214-373; L713F, V762A

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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