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pCMV4a-SIRT6-FLAG

Plasmid

#102322

Purpose

Human SIRT6 with FLAG tag for mammalian expression

Depositor

Hening Lin

Publication

Zhang et al Nat Chem Biol. 2016 Aug;12(8):614-20. do

Insert

SIRT6 (SIRT6 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q42

Plasmid

#111746

Purpose

Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

Depositor

Cheryl Arrowsmith

Publication

Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HTT (HTT Human)

Use

Baculovirus expression

Tags

FLAG

Expression

Mammalian

Mutation

Q42 (polyQ repeat)

Promoter

CMV and P10

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q19

Plasmid

#111733

Purpose

Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

Depositor

Cheryl Arrowsmith

Publication

Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HTT (HTT Human)

Use

Baculovirus expression

Tags

FLAG

Expression

Mammalian

Mutation

Q19 (polyQ repeat)

Promoter

CMV and P10

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pV1326

Plasmid

#111435

Purpose

S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR

Depositor

Gerald Fink

Publication

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgRNA-BsmBI stuffer

Use

CRISPR

Expression

Yeast

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLKO.1-puro-shFAM134B

Plasmid

#109013

Purpose

short-hairpin knockdown of targeted gene

Depositor

Jacob Corn

Publication

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

shFAM134B (RETREG1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

hU6 promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-5X-1-LARP7

Plasmid

#113545

Purpose

Produces an N-terminal GST-tagged full length LARP7 protein.

Depositor

Blerta Xhemalce

Publication

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

LARP7 (LARP7 Human)

Tags

GST

Expression

Bacterial

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAAV-hSyn-Htt171-18Q-myc-WPRE

Plasmid

#107936

Purpose

Adeno-Associated viral (AAV) vector plasmid expressing exon1 of wild type HTT (wtHTT, 18 polyQ repeats) in neurons

Depositor

Johan Jakobsson

Publication

Pircs et al Cell Rep. 2018 Aug 7;24(6):1397-1406. do

Insert

Exon 1 of wild type HTT (HTT Human)

Use

AAV

Promoter

hSynapsin

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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PB-CAG-DDdCas9VP192-T2A-GFP-IRES-Neo

Plasmid

#102885

Purpose

PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

Depositor

Timo Otonkoski

Publication

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

DDdCas9VP192-T2A-GFP-IRES-Neo

Use

CRISPR

Expression

Mammalian

Mutation

D10A, H840A

Promoter

CAG

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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PB-tight-DDdCas9VP192-T2A-GFP-IRES-Neo

Plasmid

#102889

Purpose

PiggyBac transposon system construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

Depositor

Timo Otonkoski

Publication

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

DDdCas9VP192-T2A-GFP-IRES-Neo

Use

CRISPR

Expression

Mammalian

Mutation

D10A, H840A

Promoter

TRE-tight

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGEX4T2 RANK-L

Plasmid

#108571

Purpose

Encodes C-terminal part of RANKL (aa158-aa316) with a GST N-terminal tag

Depositor

Jean-Claude Scimeca

Publication

Beranger et al J Bone Miner Res. 2007 Jul;22(7):975-83.

Insert

RANKL (receptor activator of nuclear factor kappa-B ligand) (Tnfsf11 Mouse)

Tags

GST

Expression

Mammalian

Mutation

aa158-aa316

Promoter

Ptac (trp/lac)

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSUM-Kan-MCS2-gfp

Plasmid

#109290

Purpose

Expresses high levels of gfp in mycobacteria

Depositor

Nicolai van Oers

Publication

Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

Insert

GFP

Expression

Bacterial

Mutation

WT

Promoter

Kanamycin

Available since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-Tet2 CD

Plasmid

#72219

Purpose

Express flag-tagged mouse Tet2 catalytic domain.

Depositor

Yi Zhang

Publication

Ito et al Science. 2011 Sep 2;333(6047):1300-3. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Ten-Eleven Translocation 2 (Tet2 Mouse)

Tags

flag

Expression

Mammalian

Mutation

The plasmid expresses TET2 catalytic domain.

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pcDNA3.1-STIM1-Venus-173-C

Plasmid

#87619

Purpose

Full-length STIM1 fused to C-terminal Venus (YFP) fragment; for BiFC.

Depositor

Jin Zhang

Publication

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

STIM1-Venus173-C (STIM1 Human, Synthetic)

Expression

Mammalian

Promoter

CMV

Available since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV.CAG.SF-iGluSnFR.S72A

Plasmid

#106200

Purpose

Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Publication

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.S72A

Use

AAV

Mutation

GltI: S72A

Promoter

CAG

Available since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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ptagRFP-MS2coatProtein

Plasmid

#103831

Purpose

Live cell reader for MS2 loop RNA production

Depositor

Karsten Rippe

Publication

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

MS2 coat protein (MCP) (cp )

Tags

tagRFP

Expression

Mammalian

Mutation

MCP: nt 202-240 are missing (13 aa), C242G (A81G)…

Promoter

CMV

Available since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Kif5A-CIBN

Plasmid

#102251

Purpose

Expresses fusion of kinesin heavy chain 5A (1-572) and CIB1 (1-170)

Depositor

Bianxiao Cui

Publication

Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Kif5A-CIB1 (Kif5a, CIB1 Rat, Mustard Weed)

Expression

Mammalian

Promoter

CMV

Available since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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px459 VRER

Plasmid

#101716

Purpose

sgRNA/SpCas9 expression plasmid with Cas9 VRER mutations (NGCG PAM)

Depositor

Andrew Holland

Publication

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SpCas9 VRER

Tags

3xFLAG-NLS and NLS

Expression

Mammalian

Mutation

VRER (D1135V, G1218R, R1335E and T1337R)

Available since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pC029 - Lw2Cas13a from Leptotrichia wadei F0279

Plasmid

#91919

Purpose

Expresses Lw2Cas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Publication

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

Lw2Cas13a and guide expression cassette

Use

CRISPR

Expression

Bacterial

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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p7052 pHAGE-P-CMVt-N-HA-GAW-AURKB

Plasmid

#100142

Purpose

Expreses AURKB

Depositor

Peter Howley

Publication

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

AURKB (AURKB Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCX-H2B-KikGR

Plasmid

#98649

Purpose

Expresses a nuclear-localized photoconvertible green-to-red fluorescent protein

Depositor

Anna-Katerina Hadjantonakis

Publication

Nowotschin et al BMC Dev Biol. 2009 Sep 9;9:49.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

KikGR

Tags

H2B

Expression

Mammalian

Promoter

CMV-IE enhancer chick beta actin promoter

Available since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only