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pGEX4T3-HA-ERK2 K54R

Plasmid

#53170

Purpose

Expresses GST-HA tagged human ERK2 K54R from bacterial expression vector

Depositor

Christopher Counter

Publication

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK2 (MAPK1 Mustard Weed, Human)

Tags

GST and HA

Expression

Bacterial

Mutation

K54R

Available since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pXylA-agrCA-I

Plasmid

#53437

Purpose

xylose-dependent expression of the type-I agrC and agrA genes of S. aureus

Depositor

Cynthia Collins

Publication

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

agrCA locus

Use

Synthetic Biology

Tags

none

Expression

Bacterial

Mutation

"MVQTS" sequence added to the N-terminu…

Promoter

PxylA

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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pVITRO-HPV8 L1L2

Plasmid

#52598

Purpose

Expresses HPV8 L1 and L2 in mammalian cells

Depositor

Richard Roden

Publication

Kwak et al PLoS One. 2014 May 9;9(5):e97232. doi: 1

Inserts

HPV8 L1
HPV8 L2

Expression

Mammalian

Available since

May 20, 2014

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT/DEST-3xHA/mGli2

Plasmid

#51250

Purpose

Encodes N-3xHA-TEV-mouseGli2-C

Depositor

Rajat Rohatgi

Publication

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Gli2 (Gli2 Mouse)

Use

Flpin system

Tags

3xHA-TEV

Expression

Mammalian

Promoter

EF1a

Available since

Feb. 10, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pRSM3

Plasmid

#49799

Purpose

ePathBrick vector for pathway engineering in E. coli.

Depositor

Mattheos Koffas

Publication

Xu et al ACS Synth Biol. 2012 Jul 20;1(7):256-66.

Type

Empty backbone

Tags

S-tag

Expression

Bacterial

Promoter

T7

Available since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pAraGFPCDF

Plasmid

#47516

Purpose

Contains PBAD::GFP reporter, used for heterodimer competition assay

Depositor

Bryan Berger

Publication

Su et al J Mol Biol. 2013 Jul 19. pii: S0022-2836

Insert

GFP

Expression

Bacterial

Available since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pCDNA_B2AR-no-pep

Plasmid

#47439

Purpose

control FRET sensor for beta2-adrenergic receptor

Depositor

Sivaraj Sivaramakrishnan

Publication

Malik et al J Biol Chem. 2013 Jun 14;288(24):17167-7

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

beta2-adrenergic receptor (ADRB2 Human)

Tags

10 nm ER/K helix, HA, mCerulean, mCitrine, and no…

Expression

Mammalian

Promoter

pCMV

Available since

Sept. 11, 2013

Availability

Academic Institutions and Nonprofits only
pTK96_mCherry-MRLC2

Plasmid

#46358

Depositor

Iain Cheeseman

Publication

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

MRLC2 (MYL12B Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Promoter

pBABE

Available since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCFJ1208

Plasmid

#44490

Depositor

Erik Jorgensen

Publication

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1 [Peft-3 tdTomato(NLS) tbb-2 UTR, unc-119]

Use

Gateway expression clone

Available since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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TroponinT-Gateway

Plasmid

#46026

Depositor

John Gearhart

Publication

Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

Type

Empty backbone

Use

Lentiviral

Tags

V5

Promoter

TNNT2

Available since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

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pIC113

Plasmid

#44434

Depositor

Iain Cheeseman, Arshad Desai

Publication

Cheeseman et al Sci STKE. 2005 Jan 11;2005(266):pl1.

Type

Empty backbone

Use

Retroviral

Tags

EGFP, s peptide, and tev

Expression

Mammalian

Available since

April 26, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pKT127-SEP

Plasmid

#41899

Depositor

Dan Gottschling

Publication

Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

Type

Empty backbone

Tags

superecliptic GFP (SEP)

Expression

Yeast

Available since

April 17, 2013

Availability

Academic Institutions and Nonprofits only

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pSM1960

Plasmid

#41842

Depositor

Susan Michaelis

Publication

Metzger et al J Biol Chem. 2008 Nov 21;283(47):32302-1

Insert

SEC63-mRFP

Tags

mRFP

Expression

Yeast

Available since

Jan. 22, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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FUW-TetO-Dppa2

Plasmid

#40799

Depositor

Rudolf Jaenisch

Publication

Buganim et al Cell. 2012 Sep 14;150(6):1209-22. doi: 1

Insert

Dppa2 (Dppa2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

Minimal CMV + TetO

Available since

Oct. 12, 2012

Availability

Academic Institutions and Nonprofits only
pBA2kIKmA2

Plasmid

#39213

Depositor

Anastasios Melis

Publication

Lindberg et al Metab Eng. 2010 Jan;12(1):70-9. Epub 200

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Kudzu Isoprene Synthase

Use

Synthetic Biology

Mutation

Deleted the predicted chloroplast transit peptide

Promoter

psbA2

Available since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pTK24

Plasmid

#37406

Depositor

Iain Cheeseman

Publication

Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Plk1 (PLK1 Human)

Tags

Mem-mCherry, S-tag, and TEV

Expression

Mammalian

Available since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCB169

Plasmid

#37390

Depositor

Iain Cheeseman

Publication

Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

Insert

dynein light chain TcTex-type 3 (DYNLT3 Human)

Use

Retroviral

Tags

GFP, S-tag, and TEV

Expression

Mammalian

Available since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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ss-cfSGFP2

Plasmid

#37535

Depositor

Ikuo Wada

Publication

Suzuki et al PLoS One. 2012;7(5):e37551. Epub 2012 Ma

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ss-cfSGFP2 (cysteine-free SGFP2)

Tags

signal sequence of α1-antitrypsin (M1-K34)

Expression

Mammalian

Promoter

CMV

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Myc-PSFΔN

Plasmid

#35184

Depositor

Benjamin Blencowe

Publication

Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PSF (SFPQ Human)

Tags

Myc

Expression

Mammalian

Mutation

N-terminal deletion of residues 9-264

Promoter

CMV

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pMXs-GLIS1 (FLJ33315SAAN)

Plasmid

#33274

Purpose

Retroviral expression of canonical human GLIS1

Depositor

Shinya Yamanaka

Publication

Maekawa et al Nature. 2011 . 474(7350):225-9.

Insert

GLIS1, Gateway casette A (GLIS1 Human)

Use

Retroviral

Expression

Mammalian

Available since

Dec. 14, 2011

Availability

Academic Institutions and Nonprofits only

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