We narrowed to 16,183 results for: nes

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PET-B-HiFi SpCas9-NLS-6xHis

Plasmid

#207378

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Publication

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HiFi SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

R691A, amino acids 1005-1013 replaced with two gl…

Promoter

T7

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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PET-Blackjack SpCas9-NLS-6xHis

Plasmid

#207375

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Publication

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

Blackjack SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

amino acids 1005-1013 replaced with two glycine

Promoter

T7

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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Donor_ANKRD1_KO_Hygro

Plasmid

#186668

Purpose

Donor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. eEF1a promoter, hygromycin and polyA sequence is inserted between two homology arms.

Depositor

Astrid Haase

Publication

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

ANKRD1 KO Hygromycin (ANKRD1 Human)

Expression

Mammalian

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Cas9_ANKRD1_sgRNA2

Plasmid

#186669

Purpose

Plasmid containing Cas9 and ANKRD1 sgRNA2 , sgRNA sequence: cggtcagcttatatagct.

Depositor

Astrid Haase

Publication

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

ANKRD1 KO sgRNA Plasmid (ANKRD1 Human)

Expression

Mammalian

Available since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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Nbr_C_sgRNA2

Plasmid

#186664

Purpose

Nbr C-tag sgRNA2 plasmid

Depositor

Astrid Haase

Publication

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

Nbr sgRNA 2 Plasmid (Nbr Fly)

Expression

Insect

Available since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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Nbr_C_sgRNA1

Plasmid

#186663

Purpose

Nbr C-tag sgRNA1 plasmid

Depositor

Astrid Haase

Publication

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

Nbr sgRNA 1 Plasmid (Nbr Fly)

Expression

Insect

Available since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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VIR_sgRNA

Plasmid

#186661

Purpose

VIR sgRNA plasmid

Depositor

Astrid Haase

Publication

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

VIR sgRNA Plasmid (vir Fly)

Expression

Insect

Available since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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GP807

Plasmid

#164535

Purpose

Expresses Smo noK-FLAG-Ub noK

Depositor

Gregory Pazour

Publication

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened noK (Smo Mouse)

Use

Lentiviral

Tags

FLAG_Ub noK

Mutation

All 21 cytoplasmic Lysines of Smoothened and all…

Promoter

CMV

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCbh-SpyCas9-T2A-puro-pA_U6-sgPolb (pE047)

Plasmid

#149350

Purpose

Mammalian expression vector for SpyCas9, puromycin resistance and human Polb guide RNA

Depositor

Ralf Kuehn

Publication

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Insert

SpyCas9

Use

CRISPR

Tags

Flag tag

Expression

Mammalian

Promoter

Cbh

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pBV-luc TOP2A 500bp promoter

Plasmid

#138273

Purpose

Luciferase reporter plasmid driven by TOP2A promoter

Depositor

Wen Xue

Publication

Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

Insert

TOP2A promoter sequence

Use

Luciferase

Expression

Mammalian

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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BII-ChPtW-eSpCas9

Plasmid

#133381

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Publication

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

eSpCas9

Tags

HA

Expression

Mammalian

Mutation

eSpCas9 mutations

Promoter

CMV enhancer and hEf1a (CpG free)

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCSDest2-SpCas9-MT1-NLS-3XHA-NLS

Plasmid

#69221

Purpose

Expresses R1333K mutant SpCas9 in mammalian cell

Depositor

Scot Wolfe

Publication

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

SpCas9

Use

CRISPR

Tags

NLS-3XHA-NLS

Expression

Mammalian

Mutation

Arginine 1333 is changed to Lysine

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCSDest2-SpCas9-MT2-NLS-3XHA-NLS

Plasmid

#69222

Purpose

Expresses R1333S mutant SpCas9 in mammalian cell

Depositor

Scot Wolfe

Publication

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

SpCas9

Use

CRISPR

Tags

NLS-3XHA-NLS

Expression

Mammalian

Mutation

Arginine 1333 is changed to Serine

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCSDest2-SpCas9-MT3-NLS-3XHA-NLS

Plasmid

#69223

Purpose

Expresses R1335K mutant SpCas9 in mammalian cell

Depositor

Scot Wolfe

Publication

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

SpCas9

Use

CRISPR

Tags

NLS-3XHA-NLS

Expression

Mammalian

Mutation

Arginine 1335 is changed to Lysine

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pAM057_pMi[ivory1.5kb_prom::mScarlet3-KRas6R-SV40, Pi_FibL:mBaoJin-P10UTR]

Plasmid

#261232

Purpose

Minos transgenesis construct for Lepidoptera expressing a red-fluorescent membrane tag in developing wing scales

Depositor

Arnaud Martin

Insert

ivory1.5kb_prom::mScarlet3-KRas6R-SV40, Pi_FibL:mBaoJin-P10UTR

Expression

Insect

Promoter

ivory1.5kb_prom, Pi_FibL

Available since

Aug. 31, 2026

Availability

Academic Institutions and Nonprofits only

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p_sc-eVIP-delta-Puro-EGFP-SMAD4

Plasmid

#249483

Purpose

Transfer plasmid for stable transgene expression with 2nd or 3rd generation lentiviral systems

Depositor

Nils Blüthgen

Publication

Hollek et al Mol Syst Biol. 2026 Jan 19. doi: 10.1038

Insert

SMAD4 (SMAD4 Human)

Use

Lentiviral

Promoter

EF-1a

Available since

June 16, 2026

Availability

Academic Institutions and Nonprofits only

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p_sc-eVIP-delta-Puro-EGFP-PDGFRA

Plasmid

#249474

Purpose

Transfer plasmid for stable transgene expression with 2nd or 3rd generation lentiviral systems

Depositor

Nils Blüthgen

Publication

Hollek et al Mol Syst Biol. 2026 Jan 19. doi: 10.1038

Insert

PDGFRA (PDGFRA Human)

Use

Lentiviral

Promoter

EF-1a

Available since

June 16, 2026

Availability

Academic Institutions and Nonprofits only

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p_sc-eVIP-delta-Puro-EGFP-FES-R25H

Plasmid

#249455

Purpose

Transfer plasmid for stable transgene expression with 2nd or 3rd generation lentiviral systems

Depositor

Nils Blüthgen

Publication

Hollek et al Mol Syst Biol. 2026 Jan 19. doi: 10.1038

Insert

FES (FES Human)

Use

Lentiviral

Mutation

R25H

Promoter

EF-1a

Available since

June 16, 2026

Availability

Academic Institutions and Nonprofits only

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p_sc-eVIP-delta-Puro-EGFP-CCND1

Plasmid

#249449

Purpose

Transfer plasmid for stable transgene expression with 2nd or 3rd generation lentiviral systems

Depositor

Nils Blüthgen

Publication

Hollek et al Mol Syst Biol. 2026 Jan 19. doi: 10.1038

Insert

CCND1 (CCND1 Human)

Use

Lentiviral

Promoter

EF-1a

Available since

June 9, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV_3x_U6_mmp25_CAG_nlsTagBFP2

Plasmid

#247935

Purpose

Targeting mmp25 gene in vivo by adeno-associated viral vector-mediated delivery

Depositor

Benoit Vanhollebeke

Publication

Panji et al Commun Biol. 2026 Feb 25;9(1):479. doi:

Insert

mmp25 sgRNAs (Mmp25 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

nls-TagBFP2

Expression

Mammalian

Promoter

U6

Available since

June 1, 2026

Availability

Academic Institutions and Nonprofits only

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