We narrowed to 41,529 results for: TRO

1. pETM6-Amp-T7-roGFP

   Plasmid

   #202464

   Purpose

   Expresses redox sensitive GFP (roGFP) with T7 induction

   Depositor

   Michael Lynch

   Article

   Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

   Insert

   reduction-oxidation sensitive green fluorescent protein
   

   Use

   Tags

   6X-His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX3x-hRanBP2-(IR1+M)

   Plasmid

   #53140

   Purpose

   E.coli expression of GST tagged human RanBP2 fragment with SUMO ligase activity

   Depositor

   Frauke Melchior

   Article

   Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

   Insert

   RanBP2 fragment (RANBP2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   fragment (amino acids 2631-2711)

   Promoter

   tac

   Available Since

   May 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDS277

   Plasmid

   #34973

   Purpose

   Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(V529N mutation).

   Depositor

   Michael Glotzer

   Article

   Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

   Insert

   Mid2(SS/TM)-GFP-LOVpep
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   V529N

   Promoter

   Available Since

   March 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPIDCB

   Plasmid

   #169899

   Purpose

   Empty dox inducible vector with piggyBac, insulators, and a DTS

   Depositor

   Richard Schneider

   Article

   Chu et al Biol Open. 2020 Oct 6;9(10). pii: bio.05

   Type

   Empty backbone

   Use

   Avian expression

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dox inducible

   Available Since

   Jan. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. TC513

   Plasmid

   #122616

   Purpose

   Punc-4c::SV40 NLS::rtetR::Zip(+)::SL2::mCherry

   Depositor

   Tian Chi

   Article

   Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

   Insert

   SV40 NLS::rtetR::Zip(+)::SL2::mCherry
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. L1_KanR-Ck1

   Plasmid

   #136123

   Purpose

   L1 in position 1 (next to LB in L2 construct), for KanR

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   p5-MpEF1a:nptII_3t35S
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pCk1_spacer

   Plasmid

   #136071

   Purpose

   Use when making even level constructs (L2,...) if pCk1 position (position 1) has no construct assigned

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   Odd spacer
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pBS NL4-3 envFS eGFP RT-D185K/D186L

   Plasmid

   #101332

   Purpose

   pBS NL4-3 envFS eGFP with mutation that disrupts RT catalytic activity

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   NL4-3 envFS
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   reverse transcriptase D185K/D186L

   Promoter

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT3-Neo

   Plasmid

   #69123

   Purpose

   Sleeping Beauty (SB) transposon with G418 resistance

   Depositor

   Martin Bonamino

   Article

   Chicaybam et al Front Bioeng Biotechnol. 2017 Jan 23;4:9

   Insert

   Neo (codon optimized for human)
   

   Use

   Sleeping beauty transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U3MSCV

   Available Since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET51b(+)_LAMA-G97 (for GFP)

    Plasmid

    #130715

    Purpose

    Plasmid encoding the bacterial expression of LAMA-G97 for GFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    LAMA-G97
    

    Use

    Tags

    Hisx10

    Expression

    Bacterial

    Mutation

    Deleted first Met, Ala and Last Ser from the Enha…

    Promoter

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTYF-sGFAP-IRES-tdTomato

    Plasmid

    #166920

    Purpose

    Empty plasmid backbone; includes IRES-tdTomato and sGFAP promoter

    Depositor

    Sergey Kasparov

    Article

    Vaccari Cardoso et al Glia. 2021 Jan 5. doi: 10.1002/glia.2396

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    sGFAP

    Available Since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMPMA3dPlac-PfliC-GFPLVA

    Plasmid

    #23342

    Depositor

    Olivia Steele-Mortimer

    Article

    Ibarra et al Microbiology (Reading). 2010 Apr;156(Pt

    Insert

    GFP(LVA)
    

    Use

    Tags

    GFP(LVA)

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSLQ11299: pHR-HSP-ABE8e-hyperdCas12a-EPS-puro

    Plasmid

    #210605

    Purpose

    heat shock promoter - activated hyperdCas12a-based transcriptional base editor

    Depositor

    Stanley Qi

    Article

    Liu et al Nat Commun. 2023 Oct 18;14(1):6575. doi:

    Insert

    ABE8e-hyperdCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    truncated hsp70

    Available Since

    Dec. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAGM38869

    Plasmid

    #153220

    Purpose

    Level 0 Arabidopsis U6-promoter vector

    Depositor

    Sylvestre Marillonnet

    Article

    Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

    Insert

    Arabidopsis U6 snRNA promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pALPS tat-P2A-rev

    Plasmid

    #101331

    Purpose

    Lentivector expressing codon optimized NL4-3 tat and rev linked by P2A peptide coding sequence (GSGATNFSLLKQAGDVEENPGP)

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3 tat and rev
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCB11

    Plasmid

    #201111

    Purpose

    pSR58.6 (Addgene plasmid #63176) modified to include the tetA gene upstream of sfGFP.

    Depositor

    Mary Dunlop

    Article

    Lugagne et al Nat Commun. 2024 Mar 8;15(1):2148. doi:

    Inserts

    CcaR
    tetA(C)
    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C-to-T transition at pos. no. 1049 of tetA(C) lea…

    Promoter

    J23100 and PcpcG2-172

    Available Since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pVCERC-5 Pvan-dcas9 (Sth3)

    Plasmid

    #133319

    Purpose

    expresses dcas9 from Streptococcus thermophilus #3; induced with vanillate; for homologous recombination at the vanillate locus in Caulobacter crescentus; tetracycline resistance

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    dcas9 (Streptococcus thermophilus #3)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    vanillate

    Available Since

    Jan. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTG131

    Plasmid

    #198640

    Purpose

    Thermosensitive pSC101-based plasmid expressing a sgRNA under the control of the J23119 promoter and Cas9 under the control of the DAPG-inducible PhlF promoter

    Depositor

    David Bikard

    Article

    Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pPhlF

    Available Since

    June 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. MAC-SARS-CoV-2 Orf9b

    Plasmid

    #158378

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

    Insert

    SARS-CoV-2 Orf9b
    

    Use

    Tags

    MAC-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 25, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pCAG-ChETA-mRuby2-ST

    Plasmid

    #109126

    Purpose

    Cation channelrhodopsin ChETA fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    ChETA-mRuby2-ST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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