We narrowed to 19,358 results for: Comp

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pTAMAHISTEV_PfeAQ482A

Plasmid

#128945

Purpose

expresses Q482A mutant of PfeA in E. coli

Depositor

James Naismith

Article

Moynie et al Nat Commun. 2019 Aug 14;10(1):3673. doi:

Insert

ferric enterobactin receptor

Tags

TEV protease cleavable 7xHis and TamA

Expression

Bacterial

Mutation

Signal peptide sequence has been removed (1-75), …

Promoter

T7

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF61-2xCSTREP

Plasmid

#136221

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF61

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF61

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-K1-2xCSTREP

Plasmid

#136148

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K1

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

K1

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Myc-huMEKK3 (K391A)

Plasmid

#44157

Depositor

Xin Lin

Article

Blonska et al Mol Cell Biol. 2004 Dec;24(24):10757-65.

Insert

MEKK3 (K391A) (MAP3K3 Human)

Tags

Myc

Expression

Mammalian

Mutation

K391A, kinase-dead mutant

Available Since

April 10, 2013

Availability

Academic Institutions and Nonprofits only
pCDNA3-V5-PTPN14-del-C

Plasmid

#61005

Purpose

Expresses human PTPN14 (del-phosphotase domain mutant) in mammalian cells, N-terminus V5 tag

Depositor

Jianmin Zhang

Article

Wilson et al J Biol Chem. 2014 Aug 22;289(34):23693-7

Insert

PTPN14 delete C (PTPN14 Human)

Tags

V5 tag

Expression

Mammalian

Mutation

deleted amino acids 934-1187

Promoter

CMV

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only
myc-Atg14 (1-200)

Plasmid

#86750

Purpose

N-terminal myc-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

Insert

Atg14 (ATG14 Human)

Tags

myc

Expression

Mammalian

Mutation

aa 1-200

Promoter

CMV

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-FAS-NES-jRGECO1a-WPRE

Plasmid

#141236

Purpose

AAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of jRGECO1a (jRGECO1a will not be expressed in mammalian cells that express Cre recombinase)

Depositor

Guohong Cui

Article

Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

Insert

NES-jRGECO1a

Use

AAV and Cre/Lox; Cre-off

Tags

6xHIS tag and nuclear export signal

Promoter

human elongation factor-1 alpha (EF-1 alpha)

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223-MCU-S259A

Plasmid

#31728

Depositor

Vamsi Mootha

Article

Baughman et al Nature. 2011 Jun 19;476(7360):341-5. doi

Insert

MCU (MCU Human)

Use

Pdonr gateway

Tags

None

Mutation

Changed S259 to Alanine

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only
Erk2

Plasmid

#40777

Purpose

Expression of rat Erk2 in mammalian cells

Depositor

Dustin Maly

Article

Hari et al Chem Biol. 2014 May 22;21(5):628-35. doi

Insert

Erk2 (Mapk1 Rattus norvegicus)

Tags

eGFP

Expression

Mammalian

Mutation

Starting Met removed

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only
pCDNA3-HA-HA-human CMYC (208-439)

Plasmid

#203433

Purpose

Mammalian expression of MYC

Depositor

Yoshiaki Ito

Article

Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

Insert

MYC (MYC Human)

Tags

2 x HA

Expression

Mammalian

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3−3xFLAG−AGO2(S387D)

Plasmid

#220463

Purpose

Express S387 phosphomimetic mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cells

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

Human AGO2 (AGO2 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Serine 387 to aspartate

Promoter

CMV

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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QSOX1-Tol2

Plasmid

#113383

Purpose

evaluate FOXA1 DIV motif enhancer activity near 50 kb of QSOX1 gene

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

QSOX1-enhancer (QSOX1 Human)

Use

Tol2 reporter

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO HIS HSPH3

Plasmid

#19536

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

heat shock 70kDa protein 4-like (HSPA4L Human)

Tags

His

Expression

Mammalian

Available Since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mGnptg

Plasmid

#78106

Purpose

Mammalian expression of mouse Gnptg with C-terminal His tag

Depositor

Thomas Braulke

Article

Encarnacao et al J Biol Chem. 2011 Feb 18;286(7):5311-8.

Insert

Gnptg (Gnptg Mouse)

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

May 19, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO HIS DNAJB9

Plasmid

#19559

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DnaJ (Hsp40) homolog, subfamily B, member 9 (DNAJB9 Human)

Tags

His

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pJZC32

Plasmid

#62327

Purpose

sgRNA (no RNA aptamer addition) with MCP-VP64 effector for mammalian cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA
MCP-VP64

Use

Lentiviral

Tags

VP64

Expression

Mammalian

Mutation

Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

Promoter

CMV and U6

Available Since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF63-2xCSTREP

Plasmid

#136223

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF63

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF63

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF33-2xCSTREP

Plasmid

#136194

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF33

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF33

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF60-2xCSTREP

Plasmid

#136220

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF60

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF60

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF38-2xCSTREP

Plasmid

#136199

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF38

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF38

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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