We narrowed to 13,278 results for: sequence
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Plasmid#90473PurposeElastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Term TagGFP2, and a TEV-cleavage site, leaving an N-terminal Sortase Recognition Sequence GGG and a C-terminal CysteineDepositorInsertTagGFP2-TEV-GGG-ELP(120nm)-Cys
TagsCysteine and Sortase-TagExpressionBacterialPromoterT7Available SinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS403-PGAL1-hpSC_URA3
Plasmid#22317DepositorInsertURA hairpin
UseIntegrating plasmidExpressionYeastAvailable SinceOct. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-Mito-pHCountdown
Plasmid#182302Purposelentiviral plasmid encoding pH-Countdown targeted to mitochondria via fusion to the COX8A presequence with puromycin selectionDepositorInsertmito pH countdown
UseLentiviralTagsCOX8A mitochondria targeting sequencePromoterEF-1aAvailable SinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
p350_LTJ_180bpCD90.1Template
Plasmid#104988Purpose180bp HDR template for murine CD90.1DepositorInsert180bp genomic DNA sequence from murine CD90.1
UseHdr template for gene editingAvailable SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-dSaCas9-KRAB-gRNA-TRE-blast
Plasmid#201151PurposeLentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.DepositorInsertsSadCas9-KRAB
gRNA-TRE
UseCRISPR and LentiviralTagsHAMutationgRNA sequence: ATCAGTGATAGAGAACGTATGAvailable SinceJuly 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUPD2-HR1b-C (B3-B4)
Plasmid#211851PurposeCDS for HR1b degron sequence in domestication vectorDepositorInsertHR1b
UseSynthetic BiologyAvailable SinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
p(U6b-BsaI-gRNA)
Plasmid#65956PurposeTribolium U6b promoter with BsaI cloning site to insert guide RNA sequenceDepositorTypeEmpty backboneExpressionInsectPromoterU6Available SinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJW1259
Plasmid#61251PurposeCas9 plasmid with sgRNA deleted; for use in C. elegansDepositorInsertDeletion
UseCRISPRExpressionWormMutationDeleted sgRNA(F+E) sequence from pJW1219Available SinceDec. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJYDC2
Plasmid#162459PurposeYeast display plasmid for expression of C-terminal fusions. Modification of pJYDC1 vector with ALFA tag in MCS.DepositorTypeEmpty backboneUseShuttle vector bacteria/yeastTagsALFA-tag, HA tag, HDEL sequence, and eUnaG2 fluor…Available SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
ttHsCas12a
Plasmid#182385PurposeGives high editing efficiency in barley.DepositorInsertttHsCas12a
UseCRISPR; Coding sequence onlyMutationD156RPromoterNoneAvailable SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only