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pnEA-NpM-HsGIGYF2_35-105-E46AE47FN91A-GB1_AF

Plasmid

#148686

Purpose

Bacterial Expression of HsGIGYF2_35-105-E46AE47FN91A

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF2_35-105-E46AE47FN91A (GIGYF2 Human)

Expression

Bacterial

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1-K1537SG1562EQ1658A-dsRNAres_W

Plasmid

#147860

Purpose

Insect Expression of DmNot1-K1537SG1562EQ1658A-dsRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1-K1537SG1562EQ1658A-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

10 silent mutations and two non silent N605S, K75…

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1_812-1945-dsRNAres_W

Plasmid

#147845

Purpose

Insect Expression of DmNot1_812-1945-dsRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1_812-1945-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

3 silent mutations compared to the sequence given…

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a Cdk2ap1ΔN (MT2B2)

Plasmid

#178034

Purpose

Expression vector for the purpose of protein purification.

Depositor

Lin He

Article

Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

Insert

Cdk2ap1 (Cdk2ap1 Mouse)

Use

Nonviral

Tags

6xHIS - Thrombin - MBP- TEV-TRS

Expression

Bacterial

Mutation

First N-Terminal 27aa are deleted

Promoter

T7LacI

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pLV mCgrrf1-V5

Plasmid

#175128

Purpose

Lentiviral expression of mouse Cgrrf1-V5

Depositor

Larry Gerace

Article

Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

Insert

Cgrrf1 (Cgrrf1 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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psiCheck Cdk2ap1ΔN (MT2B2)

Plasmid

#178029

Purpose

expression vector to test translation efficiency by luciferase reporter.

Depositor

Lin He

Article

Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

Insert

Cdk2ap1 (Cdk2ap1 Mouse)

Use

Luciferase; Nonviral

Tags

Luciferase

Expression

Mammalian

Promoter

Cloned in

Available Since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pNIA-CEN-FLAG-LANS1-Set2

Plasmid

#122002

Purpose

FLAG-LANS-Set2: FLAG-tagged LANS1-Set2 for light control of Set2 localization in yeast

Depositor

Brian Kuhlman

Article

Lerner et al Genome Res. 2020 Nov;30(11):1605-1617. d

Insert

histone methyltransferase SET2 (SET2 Budding Yeast)

Use

Synthetic Biology

Tags

FLAG

Expression

Yeast

Mutation

Set2 point mutations K538G, K539S, R549G, K550S, …

Promoter

ADH1

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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p35S_EVDm1

Plasmid

#167121

Purpose

Plant binary expression vector containing the coding sequence of the Arabidopsis Copia93 retroelement EVADE (AT5G17125) with U1 binding site mutated under CaMV 35S promoter.

Depositor

Arturo Marí-Ordóñez, Olivier Voinnet

Article

Oberlin et al Genome Res. 2017 Sep;27(9):1549-1562. do

Insert

EVADE (EVD) (AT5G17125 Mustard Weed)

Expression

Plant

Mutation

mutated four nucleotides at the snoRNA U1 binding…

Promoter

CaMV 35S

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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p35S_GFP-EVDinter-GUS

Plasmid

#167123

Purpose

Plant binary expression vector containing the intron and proximal polyadenylation signal sequences of the Copia93 retroelement EVADE (AT5G17125) between mGFP5 and GUS under CaMV 35S promoter.

Depositor

Arturo Marí-Ordóñez, Olivier Voinnet

Article

Oberlin et al Genome Res. 2017 Sep;27(9):1549-1562. do

Insert

EVADE GAG intron and terminator (EVD_in/ter) (AT5G17125 Mustard Weed)

Tags

GUS and mGFP5

Expression

Plant

Promoter

CaMV 35S

Available Since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-chiRNA_dCrkgRNA1

Plasmid

#131139

Purpose

Encodes gRNA targeting Drosophila crk locus. Cuts 110bp upstream of the transcription start site. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).

Depositor

Mark Peifer

Article

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Insert

dCrk gRNA1 (Crk Fly)

Use

CRISPR

Expression

Insect

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMCh-646

Plasmid

#92289

Purpose

Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 CED 7W7A with seven mutated W-motifs in D. melanogaster cells

Depositor

Marina Chekulaeva, Witold Filipowicz

Article

Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

Insert

dGW182 CED 7W7A (gw Fly)

Tags

NHA

Expression

Insect

Mutation

dGW182 CED: aa 940-1215 with the following point …

Promoter

Ac5

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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E1b-GFP-Tol2-Gateway dre SE-irf2bpl C

Plasmid

#89372

Purpose

Vector for reporter assay of one zebrafish super-enhancer region

Depositor

Alena Shkumatava

Article

Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

Insert

irf2bpl (irf2bpl Zebrafish)

Available Since

June 15, 2017

Availability

Academic Institutions and Nonprofits only

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E1b-GFP-Tol2-Gateway dre SE-irf2bpl B

Plasmid

#89371

Purpose

Vector for reporter assay of one zebrafish super-enhancer region

Depositor

Alena Shkumatava

Article

Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

Insert

irf2bpl (irf2bpl Zebrafish)

Available Since

June 15, 2017

Availability

Academic Institutions and Nonprofits only

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E1b-GFP-Tol2-Gateway dre SE-irf2bpl D

Plasmid

#89373

Purpose

Vector for reporter assay of one zebrafish super-enhancer region

Depositor

Alena Shkumatava

Article

Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

Insert

irf2bpl (irf2bpl Zebrafish)

Available Since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

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E1b-GFP-Tol2-Gateway dre SE-irf2bpl A

Plasmid

#89370

Purpose

Vector for reporter assay of one zebrafish super-enhancer region

Depositor

Alena Shkumatava

Article

Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

Insert

irf2bpl (irf2bpl Zebrafish)

Available Since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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CDK11A gRNA (BRDN0001145793)

Plasmid

#75889

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK11A

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK11A (CDK11A Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CDK11A gRNA (BRDN0001146824)

Plasmid

#75890

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK11A

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK11A (CDK11A Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CDK11A gRNA (BRDN0001147032)

Plasmid

#75891

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK11A

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK11A (CDK11A Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CDK11A gRNA (BRDN0001147530)

Plasmid

#75892

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK11A

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK11A (CDK11A Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ACVR2B gRNA (BRDN0001147208)

Plasmid

#75485

Purpose

3rd generation lentiviral gRNA plasmid targeting human ACVR2B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ACVR2B (ACVR2B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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