We narrowed to 16,983 results for: tes

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pNIC-ZB-SPRTN-Y117C-FL

Plasmid

#110219

Purpose

Expression in E. coli of full-length SPRTN protein carrying Y112A mutation, a catalytically impaired mutant, with His and ZB tags at N-terminus.

Depositor

Kristijan Ramadan

Article

Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

Insert

SPRTN (SPRTN Human)

Tags

His6 and ZB

Expression

Bacterial

Mutation

Y117C, P296L (see depositor comments below)

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA4-Tet-on-vsv-Mis12-dCEN-Incenp-GFP

Plasmid

#108485

Purpose

inducible expression of vsv-Mis12-dCEN-INCENP-EGFP

Depositor

Susanne Lens

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

Mis12-dCEN-INCENP (INCENP Human)

Tags

EGFP and VSV

Expression

Mammalian

Mutation

CEN box replaced by Mis12

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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ptdTomato-N1-CMVL-ikk1FL(C179A)-tdTomato

Plasmid

#105644

Purpose

Zebrafish Inhibitor of kappa B kinase alpha with mutation at position 179 from cysteine to alanine

Depositor

Sandra Rieger

Article

Lisse et al J Cell Sci. 2017 Mar 1;130(5):975-988. d

Insert

CMVL-ikk1FL(C179A)-tdTomato (chuk Zebrafish)

Tags

tdTomato

Expression

Mammalian

Mutation

CMV:ikk1FL(C178A)-tdTomato

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only
6xHis-TEV-GEI-17(133-509)

Plasmid

#87056

Purpose

Expresses 6xHis-tagged gei-17 (isoform f), with a TEV protease cleavage site between the tag and the ORF

Depositor

Ronald Hay

Article

Pelisch et al Nat Commun. 2014 Dec 5;5:5485. doi: 10.1

Insert

GEI-17 isfoomr f (aa 133-509) (gei-17 Nematode)

Tags

6xHis-TEV and GAGGGS linker

Expression

Bacterial

Promoter

T7

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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Cerulean(1-158) in pcDNAI/Amp

Plasmid

#60897

Purpose

This vector attaches Cerulean(1-158) to the N-terminus of a protein.

Depositor

Catherine Berlot

Article

Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E

Insert

Cerulean(1-158)

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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LXSN-Cx43-Myc

Plasmid

#60694

Purpose

retroviral expression of Myc-tagged rat Cx43

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Promoter

LTR

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
LXSN-Cx43-mAPPLE

Plasmid

#60696

Purpose

retroviral expression of rat Cx43 fused to mAPPLE

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Use

Retroviral

Tags

mAPPLE

Expression

Mammalian

Promoter

LTR

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
LXSN-Cx43-YA/VD

Plasmid

#60700

Purpose

retroviral expression of rat Cx43 YA/VD mutant

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Use

Retroviral

Expression

Mammalian

Mutation

YA/VD (Y286A/V289D)

Promoter

LTR

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
LXSN-Cx43-S279D

Plasmid

#60707

Purpose

retroviral expression of rat Cx43 S279D mutant

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Use

Retroviral

Expression

Mammalian

Mutation

S279D

Promoter

LTR

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
LXSN-Cx43-S282A

Plasmid

#60709

Purpose

retroviral expression of rat Cx43 S282A mutant

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Use

Retroviral

Expression

Mammalian

Mutation

S282A

Promoter

LTR

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
LXSN-Cx43-S282D

Plasmid

#60711

Purpose

retroviral expression of rat Cx43 S282D mutant

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Use

Retroviral

Expression

Mammalian

Mutation

S282D

Promoter

LTR

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP-WRAP53beta-N-terminus

Plasmid

#64677

Purpose

expresses only N terminal o WRAP53beta

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

WD40-encoding RNA antisense to P53 (WRAP53 Human)

Tags

GFP

Expression

Mammalian

Mutation

Only N terminus amino acids 1-160

Promoter

CMV

Available Since

May 22, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-EngPD

Plasmid

#64039

Purpose

Mammalian expression of paired domain of Pax8 fused to the N-terminal repressor domain of the drosophila engrailed protein (Eng)

Depositor

Daniel Christophe

Article

Christophe-Hobertus et al Mol Cell Endocrinol. 2007 Jan 29;264(1-2

Insert

Pax8 paired domain (Pax8 Mouse)

Tags

Eng (N-terminal repressor domain of the drosophi…

Expression

Mammalian

Mutation

paired domain, aa 1-147

Promoter

CMV

Available Since

April 23, 2015

Availability

Academic Institutions and Nonprofits only
sgFFL_3x_siRNA

Plasmid

#59893

Purpose

synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with three siRNA-like sites

Depositor

Georg Seelig

Article

Strovas et al ACS Synth Biol. 2014 May 16;3(5):324-31.

Insert

mCherry with intron containing the mouse mir-124–3

Tags

PEST and VAMP 3 UTR

Expression

Mammalian

Mutation

VAMP 3 UTR--has three siRNA-like targets (fully c…

Promoter

doxycycline (Dox)-inducible promoter (CMV/TO)

Available Since

April 1, 2015

Availability

Academic Institutions and Nonprofits only

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pCR2.1-INS-904

Plasmid

#53970

Purpose

904 bp insert from human INS gene used as a control for methylation-specific PCR assays

Depositor

Kevin Ferreri

Article

Husseiny et al PLoS One. 2012;7(10):e47942. doi: 10.137

Insert

human INS gene partial (INS Human)

Expression

Bacterial

Promoter

lac

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP-Akt-AH(R25C)

Plasmid

#39534

Depositor

Julian Downward

Article

Watton et al Curr Biol. 1999 Apr 22;9(8):433-6.

Insert

Akt(R25C) (PH domain only) (Akt1 Mouse)

Tags

6G linker, EGFP, and HA

Expression

Mammalian

Mutation

PH domain only with R25C mutation

Available Since

Sept. 26, 2012

Availability

Academic Institutions and Nonprofits only
pCS252

Plasmid

#33207

Depositor

David Bartel

Article

Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

Insert

MAP4K4 3'UTR (MAP4K4 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

miR-CGCG sites: aaCgCga, aaCgCgaa

Promoter

SV40 early enhancer/promoter

Available Since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
pEF-HA/Net1 Δ1-121

Plasmid

#28263

Depositor

Jeffrey Frost

Article

Carr et al J Biol Chem. 2009 Sep 4. 284(36):24269-8

Insert

Net1 (Net1 Mouse)

Tags

2x HA

Expression

Mammalian

Mutation

Missing aa 1-121

Available Since

April 11, 2011

Availability

Academic Institutions and Nonprofits only
ATF4 9: (SL+)5’ATF4.GFP

Plasmid

#21856

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Stem loop introduced at the 5' end of the mR…

Available Since

Sept. 22, 2009

Availability

Academic Institutions and Nonprofits only

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pCS2-mkgDUX4mqg*

Plasmid

#21175

Depositor

Stephen Tapscott

Article

Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

Insert

DUX4 (DUX4 Human)

Expression

Bacterial and Mammalian

Mutation

Point mutation at nucleotide 6163 (numbering acco…

Available Since

Aug. 6, 2009

Availability

Academic Institutions and Nonprofits only