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pmcsg7-mCRT(deltaC)

Plasmid

#83506

Purpose

Bacterial expression of Calreticulin mutant

Depositor

Malini Raghavan

Article

Del Cid et al J Biol Chem. 2010 Feb 12;285(7):4520-35.

Insert

CALR (Calr Mouse)

Tags

His

Expression

Bacterial

Mutation

delta C. Contains residues 1-339

Promoter

T7

Available Since

Oct. 12, 2016

Availability

Academic Institutions and Nonprofits only
JT504: pMVP/SB/eGFP-DEST

Plasmid

#121861

Purpose

pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ eGFP selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Sleeping beauty transposon, pmvp gateway destinat…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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JT601: pMVP/SB/mCherry-DEST

Plasmid

#121862

Purpose

pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ mCherry selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Sleeping beauty transposon, pmvp gateway destinat…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP2_1-395_V

Plasmid

#147808

Purpose

Mammalian Expression of HsDCP2_1-395

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

Insert

HsDCP2_1-395 (DCP2 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pJW1820

Plasmid

#154321

Purpose

Multi-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

GFP^SEC (LoxP)^AID*::3xFLAG

Use

CRISPR and Cre/Lox

Expression

Worm

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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KZ001: pMVP (L3-L2) P2A-eYFP + polyA

Plasmid

#121770

Purpose

pMVP L3-L2 entry plasmid, contains eYFP-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term eYFP linked by P2A to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

P2A-eYFP + polyA

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCDF11-SFPQ(276-535)

Plasmid

#135436

Purpose

Expresses SFPQ 276-535 with TEV-cleavable hexahistidine tag

Depositor

Charles Bond

Article

Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

Insert

splicing factor proline and glutamine rich (SFPQ Human)

Tags

Hexahistidine

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pBBK92 pHomL-TDH3p-Scarlet(rfp)-Link-SCS2tmh-SSA1t-HomR (AmpR)

Plasmid

#179076

Purpose

Vector containing a yeast integration cassette for expressing TDH3p-Scarlet(rfp)-Link-SCS2tmh, an overexpressed (bright) marker for the endoplasmic reticulum

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

TDH3p-Scarlet(rfp)-Link-SCS2tmh

Expression

Yeast

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK89 pHomL-TDH3p-Scarlet(rfp)-Link-2xPH-SSA1t-HomR (AmpR)

Plasmid

#179073

Purpose

Vector containing a yeast integration cassette for expressing TDH3p-Scarlet(rfp)-Link-2xPH, an overexpressed (bright) marker for the plasma membrane

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

TDH3p-Scarlet(rfp)-Link-2xPH

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK87 pHomL-RPL18Bp-TOM70-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

Plasmid

#179071

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of TOM70-Link-Scarlet(rfp), a marker for the mitochondria

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-TOM70-Link-Scarlet(rfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK85 pHomL-RPL18Bp-PEX25-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

Plasmid

#179069

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of PEX25-Link-Scarlet(rfp), a marker for the peroxisome

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-PEX25-Link-Scarlet(rfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK81 pHomL-RPL18Bp-PRC1-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

Plasmid

#179065

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of PRC1-Link-Scarlet(rfp), a marker for the vacuole

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-PRC1-Link-Scarlet(rfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK78 pHomL-RPL18Bp-SNF7-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

Plasmid

#179062

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of SNF7-Link-Scarlet(rfp), a marker for the endosome, class E compartment

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-SNF7-Link-Scarlet(rfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK77 pHomL-RPL18Bp-Scarlet(rfp)-Link-VPS21-SSA1t-HomR (AmpR)

Plasmid

#179061

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-VPS21, a marker for the endosomes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Scarlet(rfp)-Link-VPS21

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK74 pHomL-RPL18Bp-Scarlet(rfp)-Link-SFT2-SSA1t-HomR (AmpR)

Plasmid

#179058

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-SFT2, a marker for the late Golgi

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Scarlet(rfp)-Link-SFT2

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK73 pHomL-RPL18Bp-Scarlet(rfp)-Link-SED5-SSA1t-HomR (AmpR)

Plasmid

#179057

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-SED5, a marker for the early Golgi

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Scarlet(rfp)-Link-SED5

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK72 pHomL-RPL18Bp-Scarlet(rfp)-Link-SCS2tmh-SSA1t-HomR (AmpR)

Plasmid

#179056

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-SCS2tmh, a marker for the endoplasmic reticulum

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Scarlet(rfp)-Link-SCS2tmh

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK71 pHomL-RPL18Bp-ARC35-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

Plasmid

#179055

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of ARC35-Link-Scarlet(rfp), a marker for the actin cytoskeleton

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-ARC35-Link-Scarlet(rfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK69 pHomL-RPL18Bp-SPC42-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

Plasmid

#179053

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of SPC42-Link-Scarlet(rfp), a marker for the spindle pole

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-SPC42-Link-Scarlet(rfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pRP(Exp)-CMV> ORF_924bp (Azgp1):dTomato:IRES:Puro

Plasmid

#110831

Purpose

Expresses both zinc alpha-2 glycoprotein (ZAG) and dTomato (dT) reporter, where the fluorescent dT reporter is fused with the ZAG protein.

Depositor

Ande Satyanarayana

Article

Elattar et al FASEB J. 2018 Mar 23:fj201701465RR. doi:

Insert

Azgp1 (Azgp1 Mouse)

Use

Non-viral gene expression vector

Tags

dTomato

Promoter

CMV

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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