We narrowed to 13,184 results for: BASE
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Plasmid#236766PurposeA piggybac-based vector containing mouse U6 promoter-driven EIF3D sgRNA #4 and CAG promoter-driven nuclear-localized Clover-T2A-BSR.DepositorInsertEIF3D sgRNA-4 (EIF3D Human)
UsePiggybacExpressionMammalianPromotermouse U6 promoter and mouse U6 promoter, CAG prom…Available SinceMay 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT45
Plasmid#223417PurposeT-DNA vector for temperature tolerance LbCas12a-D156R based mutagenesis for dicot plants; TTTV PAM; LbCas12a-D156R and the crRNA was driven by separate 2x35s promoter; Kanamycin for plants selection.DepositorInsert2x35s-LbCas12a-D156R-2x35s-crRNA scaffold
UseCRISPRTagsNLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT46
Plasmid#223418PurposeT-DNA vector for Mb2Cas12a-RVRR based mutagenesis for monocot plants; more relaxed PAM requirements; Mb2Cas12a-RVRR and the crRNA were driven by separate ZmUbi1; BASTA for plants selection.DepositorInsertZmUbi-Mb2Cas12a-RVRR-ZmUbi-crRNA scaffold
UseCRISPRTagsNLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT47
Plasmid#223419PurposeT-DNA vector for Mb2Cas12a-RVRR based mutagenesis for monocot plants; more relaxed PAM requirements; Mb2Cas12a-RVRR and the crRNA were driven by separate ZmUbi1; Hygromycin for plants selection.DepositorInsertZmUbi-Mb2Cas12a-RVRR-ZmUbi-crRNA scaffold
UseCRISPRTagsNLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEND-303_Cacnes_tetR
Plasmid#225618PurposeC. acnes with tetR-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+TetR // P(BBa_23119-TetO2)+RBS_1+mCherryDepositorInsertTetR
UseSynthetic BiologyTags6xHisExpressionBacterialMutationCodon optimized for Cutibacterium acnesAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEND-304_Cacnes_tetR
Plasmid#225619PurposeC. acnes with tetR-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+TetR // P(BBa_23119-TetO1O3)+RBS_1+mCherryDepositorInsertTetR
UseSynthetic BiologyTags6xHis and mCherry-V5-6xHisExpressionBacterialMutationCodon optimized for Cutibacterium acnesAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEND-375_Cacnes_phlF
Plasmid#225620PurposeC. acnes with PhlF-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+PhlF // P(BBa_23119-PhlO1O3)+RBS_1+mCherry.DepositorInsertPhlF
UseSynthetic BiologyTags6xHis and mCherry-V5-6xHisExpressionBacterialMutationCodon optimized for Cutibacterium acnesAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV_Tre3g_CalcireticulinSS_mTurquoise_D4-cameleon_REAch2_KDEL
Plasmid#216253PurposeFRET-based calcium sensor located in the ER lumen, Tet-inducibleDepositorInsertER-targeted D4 second generation cameleon (FRET calcium sensor)
UseLentiviralTagsREAch and mTurquoisePromoterTRE3GAvailable SinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb12620
Plasmid#219928PurposePrTPI with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALIDepositorInsertPromoter TPI
ExpressionYeastAvailable SinceJune 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-Caprola_06
Plasmid#194667PurposeProtein production of the split HaloTag based calcium recorder Caprola_06 in bacteriaDepositorInsertCaprola_06
TagsHis-tag and Strep-tagExpressionBacterialPromoterT7Available SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-Caprola_13
Plasmid#194674PurposeProtein production of the split HaloTag based calcium recorder Caprola_13 in bacteriaDepositorInsertCaprola_13
TagsHis-tag and Strep-tagExpressionBacterialPromoterT7Available SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-Caprola_14
Plasmid#194675PurposeProtein production of the split HaloTag based calcium recorder Caprola_14 in bacteriaDepositorInsertCaprola_14
TagsHis-tag and Strep-tagExpressionBacterialPromoterT7Available SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2_RFP-60A
Plasmid#200394PurposePlasmid IVT template encoding RFP. NOTE: In the manuscript, "mCherry" was listed as the internal control for multiple fluorescence-based experiments, but it was actually RFP.DepositorInsertRFP
ExpressionMammalianAvailable SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIIS18-SapI
Plasmid#128599PurposeEntry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymesDepositorTypeEmpty backboneUseUnspecifiedAvailable SinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIIS18-BsmBI
Plasmid#128648PurposeEntry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymesDepositorTypeEmpty backboneUseUnspecifiedAvailable SinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTransgenesis p3 sv40 minimal promoter:: Katushka RFP
Plasmid#131731PurposeTransposon based transgenesisDepositorInsertSV40MinPromoter
UseXenopus expressionAvailable SinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAav-MDM2-PQS2-3xHA
Plasmid#84886PurposerAAV-based template for genome engineering of the MDM2 protein C-terminus containing PQS2 and 3xHA tags and a selection cassetteDepositorUseAAVTagsPQS2 3xHAPromoternoAvailable SinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nabi2.244
Plasmid#73756PurposeNabi2.244 is a FRET (Förster Resonance Energy Transfer)-based protein voltage sensor. This probe contains Clover and mRuby2 inserted at different locations in the Ciona voltage sensitive domain.DepositorInsertNabi2.244
ExpressionMammalianAvailable SinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAVTRE3_Clover
Plasmid#135179PurposeAAV expression of TRE(TRE3G)-driven, Clover expression for fluorescent lablingDepositorInsertsClover
TRE3G
UseAAVExpressionMammalianMutationa variant of GFPPromoterTRE3GAvailable SinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJOP-HTT-HR65Q
Plasmid#92249PurposeHTT donor DNA with 65Q for homologous recombination-mediated gene modificationDepositorInsertHTT donor DNA (HTT Human)
UsePrecisionx hr targeting vectorMutationBarcode of wobble bases to prevent TALEN cleaving…Available SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only