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pGP8A-ccdB

Plasmid

#83883

Purpose

Destination vector for integration at HIS3, expression from the ADH1 promoter

Depositor

Jennifer Nemhauser

Article

Pierre-Jerome et al Proc Natl Acad Sci U S A. 2014 Jul 1;111

Type

Empty backbone

Use

Gateway destination vector

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pXT131_TwinStrep-SUMO-ShTniQ

Plasmid

#135527

Purpose

Protein expression construct for ShTniQ

Depositor

Feng Zhang

Article

Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

Insert

ShTniQ

Tags

TwinStrep-SUMO

Expression

Bacterial

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pXT129_TwinStrep-SUMO-ShTnsB

Plasmid

#135525

Purpose

Protein expression construct for ShTnsB

Depositor

Feng Zhang

Article

Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

Insert

ShTnsB

Tags

TwinStrep-SUMO

Expression

Bacterial

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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p44K

Plasmid

#45800

Depositor

Luc Ponchon

Article

Ponchon et al Nucleic Acids Res. 2013 Aug;41(15):e150.

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only

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p18S.1 wt

Plasmid

#51727

Purpose

transcribes wild type 18S without modifications

Depositor

Vincent Mauro

Article

Burman et al Nucleic Acids Res. 2012 Sep;40(16):8085-

Insert

18S

Promoter

pol-I

Available Since

July 2, 2014

Availability

Academic Institutions and Nonprofits only

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pNSEN-d2

Plasmid

#59763

Purpose

Expresses nuclear EGFP and Tomato in mammalian cells

Depositor

Jerry Crabtree

Article

Yoo et al Nature. 2009 Jul 30;460(7255):642-6. doi

Inserts

d2-EGFP
Tomato

Tags

3X NLS and PEST

Expression

Mammalian

Promoter

CMV promoter and SV40 promoter

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pLCE-P2A-Puro

Plasmid

#123318

Purpose

Lentiviral vector for expression of eGFP-P2A-Puro cassette from a CMV promoter

Depositor

Eva Gottwein

Article

Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

Insert

eGFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pJOE6227.1

Plasmid

#135078

Purpose

An integration vector for the chromosomal deletion of the upp gene in P. putida KT2440; carries the upp deletion cassette.

Depositor

Josef Altenbuchner

Article

Graf et al Appl Environ Microbiol. 2011 Aug;77(15):

Insert

up- and downstream regions of upp (upp Pseudomonas putida)

Expression

Bacterial

Available Since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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HT-mGold

Plasmid

#183986

Purpose

Expresses fluorescent HaloTag7 fusion protein in mammalian cells in the whole cell.

Depositor

Pablo Rivera-Fuentes

Article

Emmert et al Nat Chem. 2023 Oct;15(10):1415-1421. doi

Insert

HaloTag-mGold

Tags

HaloTag, mGold

Expression

Mammalian

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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p18S.2 tag

Plasmid

#51731

Purpose

transcribes truncated transcript containing 18S and hybridization tag

Depositor

Vincent Mauro

Article

Burman et al Nucleic Acids Res. 2012 Sep;40(16):8085-

Insert

18S

Available Since

July 2, 2014

Availability

Academic Institutions and Nonprofits only

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pISH-Msx1

Plasmid

#84313

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Msx1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Msx1 (Msx1 Mouse)

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pMAL-p4X

Plasmid

#75289

Purpose

Expresses proteins in the periplasm as fusions to a high-binding mutant (A313V) maltose-binding protein

Depositor

Paul Riggs

Article

Walker et al Appl Microbiol Biotechnol. 2010 Sep;88(1

Type

Empty backbone

Tags

maltose-binding protein

Expression

Bacterial

Promoter

tac

Available Since

July 19, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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p7SKU6SBpur

Plasmid

#160329

Purpose

dual sh RNA vector expressing cfSBFP2 and a puromycin selection marker

Depositor

Ikuo Wada

Article

Matsui et al Cell Struct Funct. 2020 Jul 23;45(2):107

Type

Empty backbone

Tags

cfSBFP2

Expression

Mammalian

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCAH01Sp::Cas9D10A

Plasmid

#128161

Purpose

Ptet-controlled expression of S. pyogenes Cas9D10A variant

Depositor

Calvin Henard

Article

Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

Insert

Sp Cas9

Expression

Bacterial

Mutation

D10A

Promoter

Ptet

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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p18S.1 tag

Plasmid

#51729

Purpose

transcribes 18S containing hybridization tag

Depositor

Vincent Mauro

Article

Burman et al Nucleic Acids Res. 2012 Sep;40(16):8085-

Insert

18S

Available Since

July 2, 2014

Availability

Academic Institutions and Nonprofits only

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ECFP-Rab5C-S35N

Plasmid

#110504

Purpose

Dominant Negative

Depositor

Terence Dermody, Bernardo Mainou

Article

Mainou et al J Virol. 2012 Aug;86(16):8346-58. doi: 1

Insert

Rab5C-S35N (RAB5C Human)

Tags

ECFP

Expression

Mammalian

Mutation

S35N

Available Since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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EGFP-RILPdel212N

Plasmid

#110499

Purpose

Dominant Negative

Depositor

Terence Dermody, Bernardo Mainou

Article

Mainou et al J Virol. 2012 Aug;86(16):8346-58. doi: 1

Insert

RILPdel212N (RILP Human)

Tags

EGFP

Expression

Mammalian

Mutation

Deleted amino terminal 212 amino acids

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pISH-Otx1

Plasmid

#84315

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Otx1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Otx1 (Otx1 Mouse)

Available Since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pShuttle-FEN1mWT

Plasmid

#35032

Depositor

Sheila Stewart

Article

Saharia et al J Biol Chem. 2010 Aug 27;285(35):27057-6

Insert

FEN1mWT

Use

Adenoviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only
pSpal2At

Plasmid

#78286

Purpose

Expresses A. thaliana PAL2 in E. coli

Depositor

David Nielsen

Article

McKenna et al Metab Eng. 2011 Sep;13(5):544-54. doi: 1

Insert

PAL2 (PAL2 Mustard Weed)

Expression

Bacterial

Mutation

*see below

Promoter

lac

Available Since

June 1, 2016

Availability

Academic Institutions and Nonprofits only

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