We narrowed to 1,215 results for: calcium

1. pGP-CMV-GCaMP6s

   Plasmid

   #40753

   Purpose

   Mammalian expression of ultrasensitive protein calcium sensor

   Depositor

   Douglas Kim, GENIE Project

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Insert

   GCaMP6s
   

   Use

   Tags

   6xHis, T7 epitope, and Xpress tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-DIO {mCAR}off-{GCaMP6f}on-WPRE

   Plasmid

   #111393

   Purpose

   AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection) and Cre-ON GCaMP6f (ultrasensitive calcium sensor)

   Depositor

   Adam Kepecs

   Article

   Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

   Inserts

   mCAR (Cxadr Mouse)
   GCaMP6f
   

   Use

   AAV

   Tags

   6xHis, Myc, T7, and Xpress

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin

   Available since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGolt-mCherry / pGolt3-mCherry

   Plasmid

   #73297

   Purpose

   Visualization of Dendritic Golgi Satellite

   Depositor

   Michael Kreutz

   Article

   Mikhaylova et al Cell Rep. 2015 Dec 30. pii: S2211-1247(1

   Inserts

   ER export signal of Scap
   ER export signal of Scap
   TMD (trans-membrane domain) of Calneuron-2 (Cabp7 Rat)
   

   Use

   Tags

   ER export and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV immediate early promoter

   Available since

   Feb. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGP-CMV-GCaMP6m

   Plasmid

   #40754

   Purpose

   Mammalian expression of ultrasensitive protein calcium sensor

   Depositor

   Douglas Kim, GENIE Project

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Insert

   GCaMP6m
   

   Use

   Tags

   6xHis, T7 epitope, and Xpress tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 29, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA5/FRT_CMV_NES-Caprola_01-mEGFP

   Plasmid

   #194681

   Purpose

   CMV driven expression of the calcium recorder Caprola_01 in mammalian cell lines fused to mEGFP

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_01-mEGFP
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA5/FRT_CMV_NES-Caprola_05-mEGFP

   Plasmid

   #194683

   Purpose

   CMV driven expression of the calcium recorder Caprola_05 in mammalian cell lines fused to mEGFP

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_05-mEGFP
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA5/FRT_CMV_NES-Caprola_on-mEGFP

   Plasmid

   #194686

   Purpose

   CMV driven expression of the consitutively active calcium recorder positive control Caprola_on in mammalian cell lines fused to mEGFP

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_on-mEGFP
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA5/FRT_CMV_NES-Caprola_09-mEGFP

   Plasmid

   #194685

   Purpose

   CMV driven expression of the calcium recorder Caprola_09 in mammalian cell lines fused to mEGFP

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_09-mEGFP
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA5/FRT_CMV_NES-Caprola_off-mEGFP

   Plasmid

   #194687

   Purpose

   CMV driven expression of the inactive calcium recorder negative control Caprola_off in mammalian cell lines fused to mEGFP

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_off-mEGFP
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-Venus-cpVenus-FLARE-Cameleon

    Plasmid

    #123350

    Purpose

    Yellow-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Venus-cpVenus-FLARE-Cameleon
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGP-CMV-GCaMP6-150

    Plasmid

    #91777

    Purpose

    Mammalian expression of low affinity calcium-sensing fluorescent protein

    Depositor

    Douglas Kim, GENIE Project

    Article

    de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

    Insert

    GCaMP6-150
    

    Use

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. Tol2-elavl3-H2B-GCaMP6s

    Plasmid

    #59530

    Purpose

    Expressed nuclear-localized GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backbone

    Depositor

    Misha Ahrens

    Article

    Freeman et al Nat Methods. 2014 Jul 27. doi: 10.1038/n

    Inserts

    elavl3 promoter (elavl3 Zebrafish)
    H2B
    GCaMP6s
    

    Use

    Transposable element

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3_erGAP3

    Plasmid

    #78118

    Purpose

    Low affinity fluorescent calcium indicator of the GAP (GFP-Aequorin Protein) family targeted to the endoplasmic reticulum

    Depositor

    Teresa Alonso, Javier García-Sancho

    Article

    Navas-Navarro et al Cell Chem Biol. 2016 Jun 1. pii: S2451-9

    Insert

    erGAP3
    

    Use

    Tags

    KDEL and calreticulin signal peptide

    Expression

    Mammalian

    Mutation

    S175G, D180Y, I167V and L15Q in GFP moiety and D…

    Promoter

    CMV

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV2_hSyn_NES-Caprola_06-mEGFP_WRPE-SV40

    Plasmid

    #194690

    Purpose

    hSyn1 driven expression of the calcium recorder Caprola_06 fused to mEGFP for neuronal expression through AAV transduction

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_06-mEGFP
    

    Use

    AAV

    Tags

    mEGFP

    Expression

    Mutation

    Promoter

    hSyn1

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV2_hSyn_NES-Caprola_on-mEGFP_WRPE-SV40

    Plasmid

    #194691

    Purpose

    hSyn1 driven expression of the consitutively active calcium recorder positive control Caprola_on fused to mEGFP for neuronal expression through AAV transduction

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_on-mEGFP
    

    Use

    AAV

    Tags

    mEGFP

    Expression

    Mutation

    Promoter

    hSyn1

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV2_hSyn_NES-Caprola_off-mEGFP_WRPE-SV40

    Plasmid

    #194692

    Purpose

    hSyn1 driven expression of the inactive calcium recorder negative control Caprola_off fused to mEGFP for neuronal expression through AAV transduction

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_off-mEGFP
    

    Use

    AAV

    Tags

    mEGFP

    Expression

    Mutation

    Promoter

    hSyn1

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-51b(+)-Caprola_13

    Plasmid

    #194674

    Purpose

    Protein production of the split HaloTag based calcium recorder Caprola_13 in bacteria

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_13
    

    Use

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-mEGFP-CaMKIIa (T286D/T305A/T306A)

    Plasmid

    #127392

    Purpose

    Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286D/T305A/T306A)

    Depositor

    Ryohei Yasuda

    Article

    Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

    Insert

    calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    changed Threonine 286 to Aspartic acid and Threon…

    Promoter

    pCAG

    Available since

    July 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. CaV1.3e[8a,11,31b,Δ32,42a]

    Plasmid

    #49333

    Purpose

    Repaired Addgene #26576 to agree with the rat ref sequence. Repairs are @: nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].

    Depositor

    Diane Lipscombe

    Article

    Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

    Insert

    CACNA1D (Cacna1d Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains exons 8a, 11, 31b and 42a.Deletion of ex…

    Promoter

    CMV

    Available since

    Feb. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8m-p10

    Plasmid

    #218862

    Purpose

    Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

    Insert

    RSET-jGCaMP8m
    

    Use

    Tags

    6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

    Expression

    Insect

    Mutation

    A25G F286Y

    Promoter

    hsp70

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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