Skip to main content
Addgene
Showing: 1651 - 1680 of 13590 results

  5. pTSlb-R756S

    Plasmid
    #60730
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations R756S
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Residues 1-180 of split T7 RNAP and Residues 180-…
    Promoter
    Available since
    Jan. 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    60730-plasmid-map-sequence-id-97716

    Enlarge

  13. MT-AvrXa10-FT

    Plasmid
    #234373
    Purpose
    T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-GFP-AvrXa10-GB1) with optimized activation domain (AvrXa10), targeting Arabidopsis FT locus
    Depositor
    Michael Smanski
    Article
    Zinselmeier et al Plant Biotechnol J. 2024 Nov;22(11):3202
    Inserts
    NbGP41-GFP-AvrXa10-GB1
    dCas9-24XGP41
    gRNAs targeting FT locus
    RFP
    Use
    Synthetic Biology
    Tags
    GB1, GFP, and GP41
    Expression
    Plant
    Mutation
    Promoter
    Arabidopsis U6, Arabidopsis Ubi10, and Arabidopsi…
    Available since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    234373-plasmid-map-sequence-id-469429

    Enlarge

  15. pH-nCas9-PPE-V2

    Plasmid
    #170131
    Purpose
    For plant prime editing in rice plants or monocotyledons protoplasts
    Depositor
    Caixia Gao
    Article
    Lin et al Nat Biotechnol. 2021 Mar 25. pii: 10.103
    Insert
    nCas9(H840A)-M-MLV
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    H840A for Cas9; D200N, T306K, W313F, T330P and L…
    Promoter
    maize Ubiquitin-1, OsU3
    Available since
    May 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    170131-plasmid-map-sequence-id-333607

    Enlarge

  17. pN094

    Plasmid
    #167155
    Purpose
    MoClo-compatible Level1 (position 6) vector encoding Aspergillus nidulans 4'-phosphopantetheinyl transferase NpgA under control of 0.4 kb 35S promoter, for expression in plants
    Depositor
    Karen Sarkisyan
    Article
    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103
    Insert
    p35s-npgA-ocsT
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    p35s
    Available since
    June 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    167155-plasmid-map-sequence-id-335276

    Enlarge

  18. pSpG-PPE

    Plasmid
    #170130
    Purpose
    For plant prime editing in wheat plants or monocotyledons protoplasts
    Depositor
    Caixia Gao
    Article
    Lin et al Nat Biotechnol. 2021 Mar 25. pii: 10.103
    Insert
    nCas9-SpG(H840A)-M-MLV
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    H840A, D1135L, S1136W, G1218K, E1219Q, R1335Q, T1…
    Promoter
    maize Ubiquitin-1
    Available since
    May 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    170130-plasmid-map-sequence-id-333605

    Enlarge

  19. p2R3a-Tq2CFP-OcsT

    Plasmid
    #71268
    Purpose
    Entry clone containing Turqoise2. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system
    Depositor
    Ari Pekka Mähönen
    Article
    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246
    Insert
    Tq2CFP
    Use
    Gateway
    Tags
    4xGly linker and octaline synthase terminator
    Expression
    Mutation
    Promoter
    Available since
    Dec. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    71268-plasmid-map-sequence-id-129804

    Enlarge

  20. pTXB1-EXO1b D173A

    Plasmid
    #68269
    Purpose
    Bacterial expression of human EXO1 D173A (catalytically-dead) mutant
    Depositor
    Stefano Ferrari
    Article
    Eid et al EMBO Rep. 2010 Dec;11(12):962-8. doi: 10
    Insert
    EXO1 (EXO1 Human)
    Use
    Tags
    Mxe intein/chitin binding domain
    Expression
    Bacterial
    Mutation
    catalytically-dead D173A. Please see depositor co…
    Promoter
    T7
    Available since
    Aug. 12, 2015
    Availability
    Academic Institutions and Nonprofits only

  22. p2R3a-3xVenusYFP-OcsT

    Plasmid
    #71271
    Purpose
    Entry clone containing three repeats of Venus. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system
    Depositor
    Ari Pekka Mähönen
    Article
    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246
    Insert
    3 times VenusYFP
    Use
    Gateway
    Tags
    octaline synthase terminator
    Expression
    Mutation
    Promoter
    Available since
    Dec. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    71271-plasmid-map-sequence-id-129818

    Enlarge

  24. p2R3a-GUS-OcsT

    Plasmid
    #71267
    Purpose
    Entry clone containing the GUS enzyme. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system
    Depositor
    Ari Pekka Mähönen
    Article
    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246
    Insert
    Beta-glucuronidase
    Use
    Gateway
    Tags
    2xGly linker and octaline synthase terminator
    Expression
    Mutation
    Promoter
    Available since
    Dec. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    71267-plasmid-map-sequence-id-129805

    Enlarge

  26. pX028

    Plasmid
    #167151
    Purpose
    MoClo-compatible Level 1 (position 2) vector encoding Neonothopanus nambi hispidin synthase nnHispS under control of 0.4 kb 35S promoter, for expression in plants
    Depositor
    Karen Sarkisyan
    Article
    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103
    Insert
    p35s-nnHispS-ocsT
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    p35s
    Available since
    June 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    167151-plasmid-map-sequence-id-335280

    Enlarge

  28. pX029

    Plasmid
    #167152
    Purpose
    MoClo-compatible Level 1 (position 3) vector encoding Neonothopanus nambi hispidin-3-hydroxylase nnH3H under control of 0.4 kb 35S promoter, for expression in plants
    Depositor
    Karen Sarkisyan
    Article
    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103
    Insert
    p35s-nnH3H-ocsT
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    p35s
    Available since
    June 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    167152-plasmid-map-sequence-id-335272

    Enlarge

Showing: 1651 - 1680 of 13590 results