We narrowed to 1,755 results for: quo;

1. ins:GCaMP6s; cryaa:RFP

   Plasmid

   #110285

   Purpose

   A zebrafish insulin promoter driving the calcium indicator, GCaMP6s. Contains red eye marker, and is flanked with I-SceI sites.

   Depositor

   Nikolay Ninov

   Article

   Singh et al Nat Commun. 2017 Sep 22;8(1):664. doi: 1

   Insert

   GCaMP6s
   

   Use

   Zebrafish

   Tags

   Expression

   Mutation

   Promoter

   insulin

   Available since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-EF1a-GCaMP6s-WPRE-pGHpA

   Plasmid

   #67526

   Purpose

   "Fluorescent reporter for calcium imaging"

   Depositor

   Botond Roska

   Article

   Wertz et al Science. 2015 Jul 3;349(6243):70-4. doi:

   Insert

   GCaMP6s
   

   Use

   AAV

   Tags

   6xHis, T7 tag, and Xpress epitope

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8f-p10

   Plasmid

   #218867

   Purpose

   LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

   Depositor

   GENIE Project

   Article

   Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

   Insert

   RSET-jGCaMP8f
   

   Use

   Tags

   6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

   Expression

   Insect

   Mutation

   Q315L

   Promoter

   hsp70

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA

   Plasmid

   #68719

   Purpose

   Cre-dependent bicistronic vector expressing mRuby2 and GCaMP6f from a single open reading frame.

   Depositor

   Tobias Bonhoeffer, Mark Huebener, Tobias Rose

   Article

   Rose et al Science. 2016 Jun 10;352(6291):1319-22.

   Has Service

   AAV1

   Insert

   mRuby2-P2A-GCaMP6f
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CAG-FLEX

   Available since

   June 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGP-CMV-GCaMP3-373

   Plasmid

   #91779

   Purpose

   Mammalian expression of low affinity calcium-sensing fluorescent protein

   Depositor

   Douglas Kim, GENIE Project

   Article

   de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

   Insert

   GCaMP3-373
   

   Use

   Tags

   6xHis, T7 epitope, and Xpress tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSW505 - pOp4:3xGFP-NLS

   Plasmid

   #116005

   Purpose

   destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression

   Depositor

   Sebastien Wolf

   Article

   Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

   Insert

   pOp4:B-dummy:3xGFP:linker-NLS:tUBQ10::SulfR
   

   Use

   Tags

   linker-NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-IV-GFPL10

   Plasmid

   #98704

   Purpose

   Cre-dependently express GFPL10 fusion using Introvert Switch

   Depositor

   Alexander Nectow, Jeffrey Friedman

   Article

   Nectow et al Cell. 2017 Jul 27;170(3):429-442.e11. do

   Insert

   GFPL10
   

   Use

   AAV and Cre/Lox

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   L10: 2609 C->T to remove EcoRI site

   Promoter

   EF1a

   Available since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-SFFV-RAB10 Q68L-WPRE-UbC-Emerald

   Plasmid

   #203803

   Purpose

   Lentiviral vector plasmid expressing human RAB10 mutation Q68L (constitutively active mutant) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Richard Eva, Bart Nieuwenhuis

   Inserts

   RAB10 (RAB10 Human)
   Emerald
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Q68L

   Promoter

   Available since

   July 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8s-p10

   Plasmid

   #218865

   Purpose

   LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

   Depositor

   GENIE Project

   Article

   Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

   Insert

   RSET-jGCaMP8s
   

   Use

   Tags

   6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

   Expression

   Insect

   Mutation

   S26M F286Y Q315H

   Promoter

   hsp70

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCK314

    Plasmid

    #110545

    Purpose

    Modified (delta CRP-binding site) rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteria

    Depositor

    John Heap

    Article

    Kelly et al ACS Synth Biol. 2018 Apr 2. doi: 10.1021

    Inserts

    Modified PrhaBAD (CRP-binding sites removed)
    yfp
    rhaS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-SFFV-RAB10 T23N-WPRE-UbC-Emerald

    Plasmid

    #203802

    Purpose

    Lentiviral vector plasmid expressing human RAB10 mutation T23N (dominant negative mutant) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis

    Inserts

    RAB10 (RAB10 Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    T23N

    Promoter

    Available since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLViP-LifeAct-TagRFPT

    Plasmid

    #229700

    Purpose

    Lentiviral expression of LifeAct-TagRFPT in mammalian cells

    Depositor

    Alpha Yap

    Article

    Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

    Insert

    Synthetic construct LifeAct-mTurquoise gene
    

    Use

    Lentiviral

    Tags

    TagRFPT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV/EF1a::DIO-iLMO2

    Plasmid

    #129482

    Purpose

    AAV2 transfer plasmid for Cre-dependent expression of iLMO2, an opto-chemogenetic probe for neuronal inhibition by light or chemical

    Depositor

    Robert Gross

    Article

    Jaiswal et al J Neurosci Res. 2017 Aug 3. doi: 10.1002

    Insert

    iLMO2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV/CaMKIIa::iLMO2

    Plasmid

    #129481

    Purpose

    AAV2 transfer plasmid for iLMO2, an opto-chemogenetic probe for neuronal inhibition by light or chemical, under control of the glutamatergic-selective CaMKIIa promoter

    Depositor

    Robert Gross

    Article

    Tung et al Sci Rep. 2015 Sep 24;5:14366. doi: 10.10

    Insert

    iLMO2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-TetO(3G)-GCaMP6f-WPRE

    Plasmid

    #186205

    Purpose

    Express GCaMP6f under TetO(3G)

    Depositor

    Naoshige Uchida

    Article

    Amo et al Nat Neurosci. 2022 Aug;25(8):1082-1092.

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    6xHis (N terminal on insert), T7 epitope, and Xpr…

    Expression

    Mutation

    Promoter

    TetO(3G)

    Available since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-PGK-Venus-Fluc (puro)

    Plasmid

    #140328

    Purpose

    Lentivirus for expression of Venus-Fluc (firefly luciferase) fusion protein

    Depositor

    Roland Friedel

    Article

    Bozec et al Neurooncol Adv. 2020 Oct 10;2(1):vdaa134

    Insert

    Venus-Firefly Luciferase (luc2)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    human PGK promoter

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-FLEX-EGFPL10a

    Plasmid

    #98747

    Purpose

    Cre-dependently expresses the fusion protein EGFPL10a

    Depositor

    Alexander Nectow, Eric Schmidt, Nathaniel Heintz

    Article

    Nectow et al Cell Rep. 2017 Apr 18;19(3):655-667. doi

    Has Service

    AAV Retrograde and AAV5

    Insert

    EGFPL10a
    

    Use

    AAV and Cre/Lox

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. hCXCR4-mTQ2

    Plasmid

    #110196

    Purpose

    Encodes for human CXCR4 coding sequence tagged with the mTQ2 FP

    Depositor

    Sergi Padilla Parra

    Article

    Starling et al Nat Commun. 2023 Aug 17;14(1):4994. doi:

    Insert

    hCXCR4 (CXCR4 Human)
    

    Use

    Tags

    mTurquoise (mTQ2)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-hSyn-RiboL1-jGCaMP8s

    Plasmid

    #169247

    Purpose

    AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8s, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8s protein.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Insert

    RiboL1-jGCaMP8s
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV-hSyn-RiboL1-jGCaMP8m

    Plasmid

    #169248

    Purpose

    AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8m, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8m protein.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Insert

    RiboL1-jGCaMP8m
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. AAV-hSyn-RiboL1-jGCaMP8f

    Plasmid

    #169249

    Purpose

    AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8f, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8f protein.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Insert

    RiboL1-jGCaMP8f
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Dec. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCHMP2A-NS3-blue

    Plasmid

    #223532

    Purpose

    Mammalian expression of CHMP2A fused to the hepatitis C virus protease NS3 through a short linker containing the NS3 cleavage site, along with a blue fluorescent protein.

    Depositor

    Winfried Weissenhorn

    Article

    Wang et al Viruses. 2023 Nov 22;15(12):2289. doi: 1

    Insert

    CHMP2A (CHMP2A Human)
    

    Use

    Tags

    NS3 Hepatitis C protease and mTurquoise

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. Anti-GFP [N86/38.1R]

    Plasmid

    #114492

    Purpose

    Mammalian Expression Plasmid of anti-GFP. Derived from hybridoma N86/38.1.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCK306

    Plasmid

    #110544

    Purpose

    rhaBAD, a rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteria.

    Depositor

    John Heap

    Article

    Kelly et al ACS Synth Biol. 2018 Apr 2. doi: 10.1021

    Inserts

    PrhaBAD
    yfp
    rhaS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    N/A, PrhaBAD, and kanR promoter (upstream of kanR…

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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25. Villinpromoter-blue-FlpOERT2

    Plasmid

    #67278

    Purpose

    4hydroxytamoxifen inducible FlpO recombinase controlled by intestine specific promoter (Villin). FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon context

    Depositor

    Jannik Elverløv-Jakobsen

    Article

    Callesen et al Mol Oncol. 2017 Nov;11(11):1616-1629. do

    Insert

    mTQ2=mturquoise2 blue fluorescent gene
    

    Use

    Tags

    linked to FlpO through an P2A ribosomal skipping …

    Expression

    Mutation

    Promoter

    Villin

    Available since

    July 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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26. pGP-CMV-GCaMP6-150

    Plasmid

    #91777

    Purpose

    Mammalian expression of low affinity calcium-sensing fluorescent protein

    Depositor

    Douglas Kim, GENIE Project

    Article

    de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

    Insert

    GCaMP6-150
    

    Use

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLV-SFFV-CKAP4-WPRE-UbC-Emerald

    Plasmid

    #225943

    Purpose

    Lentiviral vector plasmid expressing human cytoskeleton associated protein 4 (CKAP4) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Evan Reid, Richard Eva, Bart Nieuwenhuis

    Inserts

    CKAP4 (CKAP4 Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV and UbC

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pH6-TEV-spTorA-GFP(C)

    Plasmid

    #169041

    Purpose

    GFP preceeded by the signal peptide of TorA (spTorA) with an N-terminal 6xHis tag and TEV cleavage site in pBAD24.

    Depositor

    Siegfried Musser

    Article

    Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

    Insert

    H6-spTorA-GFP
    

    Use

    Tags

    6xHis, TEV cleavage site, and spTorA (signal pept…

    Expression

    Bacterial

    Mutation

    The mut3 GFP variant with 5 additional mutations …

    Promoter

    araBAD

    Available since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pST_140_LVL2 cam

    Plasmid

    #179334

    Purpose

    NT-CRISPR plasmid for a single gRNA with spG Cas9 (near PAM-less Cas9 variant).

    Depositor

    Anke Becker

    Article

    Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

    Insert

    Ptac tfoX, Ptet spG cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Cas9 -> SpG Cas9 (D1135L, S1136W, G1218K, E121…

    Promoter

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGP-CMV-GCaMP6-210

    Plasmid

    #91778

    Purpose

    Mammalian expression of low affinity calcium-sensing fluorescent protein

    Depositor

    Douglas Kim, GENIE Project

    Article

    de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

    Insert

    GCaMP6-210
    

    Use

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge