We narrowed to 7,555 results for: ALP

1. pCAGGS-mNG-eDHFR(69K6)-G-alpha-q(L254A)

   Plasmid

   #178851

   Purpose

   Mammalian expression of Gαq(L254A) fused to mNeonGreen-eDHFR(69K6)

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   mNeonGreen-eDHFR(69K6)-Gαq(L254A) (GNAQ Human)
   

   Expression

   Mammalian

   Mutation

   Gαq: C9S/C10S/Q209L/L254A

   Promoter

   CAG

   Available Since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. p(HA)HIF1alpha(401delta603) (L795V, C800S, L818S, L822V)

   Plasmid

   #52216

   Purpose

   mammalian expression of HA tagged HIF1alpha with a deletion of the N-terminal activation domain and LCLL mutation

   Depositor

   Eric Huang

   Article

   Koshiji et al EMBO J. 2004 May 5;23(9):1949-56. Epub 2

   Insert

   HIF1alpha (401delta603) LCLL (HIF1A Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   aa 401-603 deleted, removes the oxygen-dependent …

   Promoter

   CMV

   Available Since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

3. V308 pDNR MCS lacZ alpha - Donor

   Plasmid

   #11691

   Depositor

   Tony Pawson

   Article

   Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

   Type

   Empty backbone

   Use

   Cre/Lox; Donor vector

   Available Since

   May 24, 2006

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pCMV5-Flag-EPLIN alpha delta LIM

   Plasmid

   #40933

   Depositor

   Scott Maul, Elizabeth Luna

   Article

   Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

   Insert

   EPLIN alpha (LIMA1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   LIM domain deleted- amino acids 221-295

   Available Since

   Dec. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pU6-mir144 EF1Alpha-puro-T2A-BFP

   Plasmid

   #164791

   Purpose

   Express miR-144 under the U6 promoter in mammalian cells

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Inserts

   miR-144 (MIR144 Human)
   puro-T2A-BFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1Alpha and U6

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV5-Flag-EPLIN alpha delta C-term

   Plasmid

   #40930

   Depositor

   Scott Maul, Elizabeth Luna

   Article

   Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

   Insert

   EPLIN alpha (LIMA1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   deleted amino acids 296-600

   Available Since

   Dec. 12, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH1

   Plasmid

   #164800

   Purpose

   For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA1

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Inserts

   ERH CRISPRi gRNA1
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1Alpha and U6

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH2

   Plasmid

   #164801

   Purpose

   For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA2

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Inserts

   ERH CRISPRi gRNA2
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1Alpha and U6

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Venus Cam KII alpha mutant T286D

   Plasmid

   #29429

   Depositor

   Steven Vogel

   Article

   Thaler et al Proc Natl Acad Sci U S A. 2009 Apr 14. 1

   Insert

   Venus CAM KII Alpha
   

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   T286D mutation in CamKIIa sequence

   Available Since

   July 5, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pALPS_3'LTR GFP@gag start SFFV-

    Plasmid

    #101337

    Purpose

    Repaired U3 allows LTR-based transcription by the provirus with GFP as a marker for expression. WPRE was deleted.

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. Venus Cam KII alpha mutant T286A

    Plasmid

    #29430

    Depositor

    Steven Vogel

    Article

    Thaler et al Proc Natl Acad Sci U S A. 2009 Apr 14. 1

    Insert

    Venus CAM KII Alpha
    

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    T286A mutation in CamKIIa sequence

    Available Since

    June 3, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pDGB3 alpha1 Tnos:nptII:Pnos - SF - P35S:hCas9:Tnos (GB1656)

    Plasmid

    #160618

    Purpose

    Module for the expression of the human codon optimized Cas9 and kanamycin resistance (NptII).

    Depositor

    Diego Orzaez

    Insert

    Tnos:NptII:Pnos-SF-P35s:hCas9:tNos
    

    Use

    CRISPR

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Pnos, 35S

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA Flag-RARalpha (T181A,S445A,S461A)

    Plasmid

    #35556

    Depositor

    Jonathan Kurie, Harish Srinivas

    Article

    Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

    Insert

    RARalpha (RARA Human)
    

    Tags

    Flag and His

    Expression

    Mammalian

    Mutation

    T181A, S445A, S461A

    Available Since

    April 5, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pENTR/D-Topo genomic A. thaliana IMPORTIN ALPHA 1

    Plasmid

    #175814

    Purpose

    pENTR plasmid with genomic sequence of Arabidopsis thaliana IMPORTIN ALPHA ISOFORM 1 (AT3G06720) without stop codon for C-terminal epitope tags

    Depositor

    Lennart Wirthmueller, Mark Banfield

    Article

    Wirthmueller et al Plant J. 2015 Jan;81(1):40-52. doi: 10.1

    Insert

    IMPORTIN ALPHA ISOFORM 1 (IMPA-1 Mustard Weed)
    

    Use

    Pentr plasmid for gateway cloning

    Tags

    none

    Promoter

    none

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBSSK p85 alpha (delta SH3, iSH2)

    Plasmid

    #13433

    Depositor

    Ronald Kahn

    Article

    Taniguchi et al Proc Natl Acad Sci U S A. 2006 Aug 8. 10

    Insert

    p85 alpha (delta SH3, iSH2) (PIK3R1 Human)
    

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Flag tags replacing both the SH3 and iSH2 domains.

    Available Since

    Nov. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pJM450 EF1alpha EBFP2-P2A-BlastR (TUPV1)

    Plasmid

    #139230

    Purpose

    mMoClo TUPV1, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    EBFP2-P2A-Blasticidin S Deaminase
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Danio alpha Bb-crystallin 1kb promoter/AcGFP

    Plasmid

    #98098

    Purpose

    aBb-crystallin promoter fragment in GFP plasmid to assess activity in larval embryos

    Depositor

    Mason Posner

    Article

    Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

    Insert

    Alpha Bb-crystallin promoter 1 kb fragment, Danio rerio (cryabb Zebrafish)
    

    Use

    Gfp expressing

    Tags

    GFP

    Promoter

    Danio aBb-crystallin 1 kb fragment (-1074/-1)

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. Danio alpha Ba-crystallin 3kb promoter/AcGFP

    Plasmid

    #98097

    Purpose

    aBA-crystallin promoter fragment in GFP plasmid to assess activity in larval embryos

    Depositor

    Mason Posner

    Article

    Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

    Insert

    Alpha Ba-crystallin promoter 3 kb fragment, Danio rerio (cryaba Zebrafish)
    

    Use

    Gfp expressing

    Tags

    GFP

    Promoter

    Danio alpha Ba-crystallin 3 kb fragment (-3000/-1)

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSG5-FLAG-CaMKK alpha with CaMKKbeta RP domain

    Plasmid

    #33331

    Depositor

    Anthony Means

    Article

    Green et al Cell Signal. 2011 Dec;23(12):2005-12. Ep

    Insert

    Calcium/Calmodulin dependent protein kinase kinase alpha (Camkk1 Rat)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    CaMKKalpha RP domain (167-189) was replaced with …

    Promoter

    SV40

    Available Since

    April 10, 2012

    Availability

    Academic Institutions and Nonprofits only

20. V677 pDNR MCS BE lacZ alpha - Donor

    Plasmid

    #11694

    Depositor

    Tony Pawson

    Article

    Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

    Type

    Empty backbone

    Use

    Cre/Lox; Donor vector

    Available Since

    May 24, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge