We narrowed to 2,816 results for: cdna expression plasmid
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Plasmid#204695PurposeThis plasmid allows for inducible expression of BRD4-NUT(MIN) mutant fusion tagged with mNeonGreen, in mammalian cells.DepositorTagsmNeonGreenExpressionMammalianMutationdeletion of majority of NUT, retaining only amino…PromoterCMVAvailable SinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only
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pcDNA5-FRT/TO-mNeonGreen-BRD4-p300(deltaI)
Plasmid#204701PurposeThis plasmid allows for inducible expression of engineered BRD4-p300(I) fusion tagged with mNeonGreen, in mammalian cells.DepositorTagsmNeonGreenExpressionMammalianMutationdeletion of intrinsically disordered regions of p…PromoterCMVAvailable SinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2 mir-1-1 reporter hsa-mir-133a-1
Plasmid#46676DepositorInserthsa-mir-133a-1 (MIR133A1 Human)
UseRNAiTagsV5 and hsa-mir-1-1ExpressionMammalianPromoterCMVAvailable SinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2 mir-1-1 reporter hsa-mir-122
Plasmid#46673DepositorInserthsa-mir-122 (MIR122 Human)
UseRNAiTagsV5 and hsa-mir-1-1ExpressionMammalianPromoterCMVAvailable SinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2 mir-1-1 reporter hsa-mir-138-2
Plasmid#46677DepositorInserthsa-mir-138-2 (MIR138-2 Human)
UseRNAiTagsV5 and hsa-mir-1-1ExpressionMammalianPromoterCMVAvailable SinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2 mir-1-1 reporter hsa-mir-205
Plasmid#46679DepositorInserthsa-mir-205 (MIR205 Human)
UseRNAiTagsV5 and hsa-mir-1-1ExpressionMammalianPromoterCMVAvailable SinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2 mir-1-1 reporter hsa-mir-142
Plasmid#46678DepositorInserthsa-mir-142 (MIR142 Human)
UseRNAiTagsV5 and hsa-mir-1-1ExpressionMammalianPromoterCMVAvailable SinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
cDNA5-FRT/TO-APT2-LAMA-F98
Plasmid#234427PurposeExpress APT2 with a C terminal fusion to the GFP enhancer nanobody containing dihydrofolate reductase inserted in the nanobody at position F98DepositorInsertAPT2 fused to the GFP enhancer nanobody containing dihydrofolate reductase inserted in the nanobody at position F98 (LYPLA2 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable SinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-APT2-LAMA-G97
Plasmid#234426PurposeExpress APT2 with a C terminal fusion to the GFP enhancer nanobody containing dihydrofolate reductase inserted in the nanobody at position G97DepositorInsertAPT2 fused to the GFP enhancer nanobody containing dihydrofolate reductase inserted in the nanobody at position G97 (LYPLA2 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable SinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-CRY2-mCh-superPLDx100-P2A-CIBN-CAAX
Plasmid#188994PurposeA plasmid for dual expression of CRY2-mCh-superPLDx100 (an engineered PLD mutant with 100 times higher activity than the wild-type) and plasma membrane-tagged CIBNDepositorInsertPLD
TagsCAAX, CIBN, CRY2, P2A, and mCherryExpressionMammalianMutationK57R, A59V, K109R, P245A, V264I, G328S, G381V, G4…PromoterCMVAvailable SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-CRY2-mCh-superPLDx10-P2A-CIBN-CAAX
Plasmid#188991PurposeA plasmid for dual expression of CRY2-mCh-superPLDx10 (an engineered PLD mutant with 10 times higher activity than the wild-type) and plasma membrane-tagged CIBNDepositorInsertPLD
TagsCAAX, CIBN, CRY2, P2A, and mCherryExpressionMammalianMutationF163L, K249R, G328S, G381V, G429D, D480GPromoterCMVAvailable SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-CRY2-mCh-superPLDx30-P2A-CIBN-CAAX
Plasmid#188992PurposeA plasmid for dual expression of CRY2-mCh-superPLDx30 (an engineered PLD mutant with 30 times higher activity than the wild-type) and plasma membrane-tagged CIBNDepositorInsertPLD
TagsCAAX, CIBN, CRY2, P2A, and mCherryExpressionMammalianMutationK109R, P245A, G328S, G381V, G429DPromoterCMVAvailable SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-CRY2-mCh-superPLDx50-P2A-CIBN-CAAX
Plasmid#188993PurposeA plasmid for dual expression of CRY2-mCh-superPLDx50 (an engineered PLD mutant with 50 times higher activity than the wild-type) and plasma membrane-tagged CIBNDepositorInsertPLD
TagsCAAX, CIBN, CRY2, P2A, and mCherryExpressionMammalianMutationD201G, A258T, K327R, G328S, G381V, G429D, T450APromoterCMVAvailable SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CAG-NanoLuc-VChR1-EYFP
Plasmid#114112Purposefusion protein of NanoLuc, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogeneticsDepositorInsertNanoLuc; Volvox channelrhodopsin 1; EYFP
ExpressionMammalianPromoterCAGAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2 mir-1-1 reporter hsa-mir-128-1
Plasmid#46675DepositorInserthsa-mir-128-1 (MIR128-1 Human)
UseRNAiTagsV5 and hsa-mir-1-1ExpressionMammalianPromoterCMVAvailable SinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
(363) pcDNA3.1-Nup62-Ubc-Flag-EPEA
Plasmid#168098PurposeNucleoporin Nup62 with C-terminal Ubc nanobody tag, Flag tag, and EPEA nanobody tagDepositorInsertNup62-Ubc-Flag-EPEA
TagsEPEA-tag, Flag-tag, and Ubc-TagExpressionMammalianMutationP229A, S283T on Nup62 native sequencePromoterT7/cmvAvailable SinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 frt/to N-BioTAP-C-BRD4-NUT
Plasmid#171630PurposeExpresses BioTAP-tagged BRD4-NUT in mammalian cells. Can be inserted into a FRT site by co-transfection with a plasmid encoding Flp recombinaase.DepositorAvailable SinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 CMV 3xFG eIF4G1 +MIC
Plasmid#158776PurposepcDNA5 transfection plasmid for the exogenous expression of N-terminal 3xFlag-tagged eIF4G1 isoform including the microexon.DepositorInserteIF4G1 (with microexon)
Tags3xFlagExpressionMammalianAvailable SinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EFNB2-RBD-Fc (D62Q-Q130L-V167L)
Plasmid#200980PurposeMammalian expression plasmid for soluble EFNB2 (D62Q-Q130L-V167L specificity mutant) fused to IgG1 FcDepositorInsertEFNB2 (EFNB2 Human)
TagsIgG1 FcExpressionMammalianMutationSoluble Receptor-Binding Domain; D62Q-Q130L-V167L…PromoterCMVAvailable SinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-myc:Skp1(dC111):gs6:TEVs:gs6:fLuc:HA
Plasmid#228530PurposeFirefly luciferase in fusion with a fragment of human Skp1 protein through a flexible linker and a TEV viral protease cleavage site. Expression of fLuc protein is deinhibited by TEV site cleavage.DepositorInsertmyc:Skp1(dC111):gs6:TEVs:gs6:fLuc:HA (SKP1 Human, Synthetic, Photinus pyralis)
TagsHA and mycExpressionMammalianPromoterCMVAvailable SinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only