We narrowed to 2,181 results for: levi

1. pXJ197-pAAV-PB3'IR-U6-gRNA-directcapture-EF1a-GFP-KASH-PB5'IR

   Plasmid

   #210523

   Purpose

   AAV piggybac transposon vector, with GFP-KASH reporter and gRNA cloning site. Compatible with 10X 3' and 5' gRNA capture technology.

   Depositor

   Xin Jin

   Article

   Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

   Inserts

   GFP-KASH
   U6-gRNA-directcapture
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFPC1-hVAP-A KD/MD

   Plasmid

   #104449

   Purpose

   Expression of human VAP-A (mutant K94D/M96D, unable to bind FFAT motifs) fused to GFP in mammalian cells

   Depositor

   Catherine Tomasetto

   Article

   Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

   Insert

   vesicle-associated membrane protein-associated protein A (VAPA Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   changed K94 to D and M96 to D

   Promoter

   Available since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFB1.HMBP.PrS.EZH2

   Plasmid

   #125161

   Purpose

   Expresses human EZH2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

   Depositor

   Chen Davidovich

   Article

   Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

   Insert

   EZH2 (EZH2 Human)
   

   Use

   Baculovirus expression vector

   Tags

   His-MBP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFPC1-hVAP-B KD/MD

   Plasmid

   #104450

   Purpose

   Expression of human VAP-B (mutant K87D/M89D, unable to bind FFAT motifs) fused to GFP in mammalian cells

   Depositor

   Catherine Tomasetto

   Article

   Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

   Insert

   vesicle-associated membrane protein-associated protein B (VAPB Human)
   

   Use

   Tags

   EGFP and HA

   Expression

   Mammalian

   Mutation

   changed K87 to D and M89 to D

   Promoter

   Available since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFB1.HMBP.PrS.RBBP4

   Plasmid

   #125164

   Purpose

   Expresses human RBBP4 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

   Depositor

   Chen Davidovich

   Article

   Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

   Insert

   Histone-binding protein RBBP4 (RBBP4 Human)
   

   Use

   Tags

   His-MBP

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-N-HA-NEK7 K64M

   Plasmid

   #75143

   Purpose

   Expresses N-terminal HA-tagged NEK7 (with a K64M substitution; kinase dead) in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

   Insert

   NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Lys 64 mutated to Met; kinase inactive and F236L …

   Promoter

   CMV

   Available since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. BPV00500 pET21GG2 His(6)-TF-BECN(1-450)

   Plasmid

   #136684

   Purpose

   Expresses the full-length Beclin-1 protein (a.a. 1-450) with a N-terminal hexa-Histidine and solubility tag (i.e., Trigger Factor)

   Depositor

   Colin Garvie

   Article

   Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-66

   Insert

   Beclin-1 (BECN1 Human)
   

   Use

   Tags

   Trigger Factor

   Expression

   Bacterial

   Mutation

   DNA optimized for E. coli expression

   Promoter

   T7

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAC1-V16-Luc

   Plasmid

   #122034

   Purpose

   AAV vector, pTet bidi (bidirectional tetracycline-responsive promoter) driving Tet-dependent rtTA and Luciferase expression

   Depositor

   Liliane Tenenbaum

   Article

   Chtarto et al Mol Ther Methods Clin Dev. 2016 Mar 30;5

   Insert

   Luciferase
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGL3-Basic-Rab18-sfGFP(N) HDR template

   Plasmid

   #129415

   Purpose

   HDR tempalte for tagging of endogenous human RAB18 N-terminus with sfGFP

   Depositor

   Elina Ikonen

   Article

   Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

   Insert

   RAB18 HDR template (RAB18 Human)
   

   Use

   CRISPR and TALEN; Endogenous tagging hdr template

   Tags

   sfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFB1.HMBP.PrS.AEBP2

    Plasmid

    #125165

    Purpose

    Expresses human AEBP2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

    Depositor

    Chen Davidovich

    Article

    Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

    Insert

    Zinc finger protein AEBP2 (AEBP2 Human)
    

    Use

    Tags

    His-MBP

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFB1.HMBP.PrS.EED

    Plasmid

    #125163

    Purpose

    Expresses human EED in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

    Depositor

    Chen Davidovich

    Article

    Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

    Insert

    Polycomb protein EED (EED Human)
    

    Use

    Tags

    His-MBP

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFB1.HMBP.PrS.SUZ12

    Plasmid

    #125162

    Purpose

    Expresses human SUZ12 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

    Depositor

    Chen Davidovich

    Article

    Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

    Insert

    Polycomb protein SUZ12 (SUZ12 Human)
    

    Use

    Baculovirus expression vector

    Tags

    His-MBP

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLenti CMV Puro DHFR.dn-cHSF1

    Plasmid

    #134729

    Purpose

    Lentiviral expression vector for constitutively active dominant-negative HSF1 fused to DHFR destabilized domain

    Depositor

    Matthew D. Shoulders

    Article

    Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

    Insert

    DHFR.dn-cHSF1 (HSF1 Human)
    

    Use

    Lentiviral

    Tags

    DHFR

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 186-202, 379−529

    Promoter

    CMV

    Available since

    Aug. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFBOH-MHL.SUZ12

    Plasmid

    #125172

    Purpose

    Expresses human SUZ12 in insect cells, under a TEV-cleavable N-terminal polyhistidine tag.

    Depositor

    Chen Davidovich

    Article

    Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

    Insert

    SUZ12 (SUZ12 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. BPV00554 pET21GG2-His(6)-BECN(248-450)

    Plasmid

    #136685

    Purpose

    Expresses the Beclin-1 ECD/BARA domain (a.a. 248-450) with a N-terminal hexa-Histidine tag

    Depositor

    Colin Garvie

    Article

    Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-66

    Insert

    Beclin-1 ECD/BARA (BECN1 Human)
    

    Use

    Tags

    Hexa-Histadine

    Expression

    Bacterial

    Mutation

    DNA optimized for E. coli expression

    Promoter

    T7

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMXs-IRES-blast 3xFlag-SPNS1(E164K)

    Plasmid

    #198569

    Purpose

    SPNS1(E164K) codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vector

    Depositor

    Monther Abu-Remaileh

    Article

    Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

    Insert

    SPNS1(E164K) with n-terminal 3xFlag (SPNS1 Human)
    

    Use

    Retroviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    changed glutamic acid E164 to lysine (E164K)

    Promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. BPV00605 pET21GG2-His(6)-BECN(1-265)

    Plasmid

    #136686

    Purpose

    Expresses the Beclin-1 IDR+CC domains (a.a. 1-265) with a N-terminal hexa-Histidine tag

    Depositor

    Colin Garvie

    Article

    Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-66

    Insert

    Beclin-1 IDR + CC (BECN1 Human)
    

    Use

    Tags

    Hexa-Histadine

    Expression

    Bacterial

    Mutation

    DNA optimized for E. coli expression

    Promoter

    T7

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pU6-crRNA(TARDBP)

    Plasmid

    #109323

    Purpose

    AAV vector expressing crRNA for AsCpf1 (RR variant) targeting human TARDBP

    Depositor

    Feng Zhang

    Article

    Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

    Insert

    mCherry-KASH (TARDBP Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn1

    Available since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-ITR-CMV-mCherry-1xU6-LeuIGI1

    Plasmid

    #217367

    Purpose

    Expresses E. coli leucine tRNA variant "LeuIGI1" and a wild-type mCherry reporter; can be packaged into AAV

    Depositor

    Abhishek Chatterjee

    Article

    Huang et al ACS Synth Biol. 2024 Jul 19;13(7):2141-2

    Insert

    E. coli leucine tRNA mutant, "LeuIGI1" for TAG suppression
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    G6U, C78G, U83C

    Promoter

    U6

    Available since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-ITR-CMV-mCherry-1xU6-LeuIGI2

    Plasmid

    #217368

    Purpose

    Expresses E. coli leucine tRNA variant "LeuIGI2" and a wild-type mCherry reporter; can be packaged into AAV

    Depositor

    Abhishek Chatterjee

    Article

    Huang et al ACS Synth Biol. 2024 Jul 19;13(7):2141-2

    Insert

    E. coli leucine tRNA mutant, "LeuIGI2" for TAG suppression
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    C2G, C3G, G6U, A7G, U77C, C78G, G81C, G82C, U83C

    Promoter

    U6

    Available since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge