We narrowed to 1,639 results for: plasmid for e coli
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Plasmid#92227PurposeExpression of deGFP mutant Y35TAG D193TAG for ncAA incoporation using the p70a promoter and UTR1DepositorInsertdeGFP Y35TAG D193TAG
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialMutationResidues Y35 D193 were mutated into TAG (Amber) s…Promoterp70aAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR3-Nterm 6xHis mRFP K15TAG-T500
Plasmid#92319PurposeExpression of NTerm 6xHis tagged mRFP mutant K15TAG for ncAA incoporation using the p70a promoter and UTR3DepositorInsertOptimized NTerm 6xHis tagged monomeric RFP (mRFP)
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialMutationPromoterp70aAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR1-Nterm 6xHis mRFP K15TAG-T500
Plasmid#92318PurposeExpression of NTerm 6xHis tagged mRFP mutant K15TAG for ncAA incoporation using the p70a promoter and UTR1DepositorInsertOptimized NTerm 6xHis tagged monomeric RFP (mRFP)
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialMutationPromoterp70aAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEC2483 (pSpy1C)
Plasmid#220248Purposereplicative E. coli-S. pyogenes shuttle plasmid for heterologous gene expressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQE80_T5_Z17-ehaA_LacI
Plasmid#186313PurposePlasmid for inducible E coli expression of the Z17 helixCAM.DepositorInsertZ17-ehaA
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQE80_T5_Z18-ehaA_LacI
Plasmid#186314PurposePlasmid for inducible E coli expression of the Z18 helixCAM.DepositorInsertZ18-ehaA
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQE80_T5_P3-ehaA_LacI
Plasmid#186315PurposePlasmid for inducible E coli expression of the P3 helixCAM.DepositorInsertP3-ehaA
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQE80_T5_AP4-ehaA_LacI
Plasmid#186316PurposePlasmid for inducible E coli expression of the AP4 helixCAM.DepositorInsertAP4-ehaA
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQE80_T5_P9-ehaA_LacI
Plasmid#186317PurposePlasmid for inducible E coli expression of the P9 helixCAM.DepositorInsertP9-ehaA
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQE80_T5_AP10-ehaA_LacI
Plasmid#186318PurposePlasmid for inducible E coli expression of the AP10 helixCAM.DepositorInsertAP10-ehaA
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSc1-DD
Plasmid#80439PurposeExpresses eGFP with ecDHFR along with an U6 promoter driven gRNA which cleaves the plasmid in-cellDepositorInsertssp Cas9 gRNA
eGFP
UseCRISPRTagsE. coli dihydrofolate reductaseExpressionMammalianMutationPromoterCMV and U6Available sinceAug. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xEcoLeuT(CUA)_AnapRS
Plasmid#140019PurposePlasmid with 4xEcoLeuT(CUA) cassette and E. coli AnapRS for amber suppression and incorporation of the fluorescent ncAA Anap; for transient or stable piggyBac-mediated integrationDepositorInsertEcoLeuRS (AnapRS)
UseTagsExpressionMammalianMutationL38F, M40G, L41P, Y499V, Y500L, Y527A, H537E, L53…PromoterEF1Available sinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEAT8-137 M1V
Plasmid#173807PurposeExpression and purification of mature (no signal sequence) human alpha1-antitrypsin wildtype variant M1V (UniProt P01009) from E. coli BL21(DE3) cellsDepositorInsertSERPiNA1 (SERPINA1 Human)
UseTagsExpressionBacterialMutationE1M Otherwise this plasmid encodes the most commo…PromoterT7Available sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEcNucC
Plasmid#214050PurposeBacterial expression plasmid for E. coli MS 115-1 NucC, a cA3-responsive nuclease known to cause abortive infection; used as a positive control in the Haliangium ochraceum type III CRISPR-Cas systemDepositorInsertEcNucC
UseTagsExpressionBacterialMutationWTPromoterlacUV5 promoterAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
MP-P
Plasmid#69661PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926 dam seqA emrR mutH(E56A)
UseTagsExpressionBacterialMutationPromoterE. coli PBADAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
MP-U
Plasmid#69699PurposeUntargetted Bacterial MutagenesisDepositorInsertRBS+ dnaQ926 dam seqA emrR ugi CDA1
UseTagsExpressionBacterialMutationPromoterE. coli PBADAvailable sinceSept. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
MP-T6
Plasmid#69698PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926 dam seqA emrR ugi AID(Mut7.3.2)
UseTagsExpressionBacterialMutationPromoterE. coli PBADAvailable sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
MP-R6
Plasmid#69689PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926 dam seqA emrR ugi CDA1 (opt)
UseTagsExpressionBacterialMutationPromoterE. coli PBADAvailable sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
MP-P9
Plasmid#69668PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926 dam seqA emrR ugi APOBEC1
UseTagsExpressionBacterialMutationPromoterE. coli PBADAvailable sinceNov. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
MP-P8
Plasmid#69667PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926 dam seqA emrR ugi AID
UseTagsExpressionBacterialMutationPromoterE. coli PBADAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only