We narrowed to 17,163 results for: EMB

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nAChR beta2 WT

Plasmid

#24272

Depositor

Henry Lester

Article

Nashmi et al J Neurosci. 2003 Dec 17. 23(37):11554-67

Insert

nAChR beta2 WT (Chrnb2 Mouse)

Expression

Mammalian

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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pEGFP SNAP47

Plasmid

#101915

Purpose

Expresses SNAP47 in mammalian cells

Depositor

Thierry Galli

Article

Kuster et al J Biol Chem. 2015 Nov 20;290(47):28056-6

Insert

SNAP47 (SNAP47 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2018

Availability

Academic Institutions and Nonprofits only

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nAChR alpha4 WT

Plasmid

#24271

Depositor

Henry Lester

Article

Nashmi et al J Neurosci. 2003 Dec 17. 23(37):11554-67

Insert

nAChR alpha4 WT (Chrna4 Mouse)

Expression

Mammalian

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only
pME Actin Vhh mCherry (JDW 1240)

Plasmid

#224499

Purpose

Gateway compatible middle entry clone containing an Actin nanobody fused to mCherry (For visualizing actin, actin chromobody)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Actin-Vhh-mCherry

Use

Gateway cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221_HDLBP

Plasmid

#154448

Purpose

For recombination of HDLBP

Depositor

Markus Landthaler

Article

Zinnall et al Nat Commun. 2022 May 18;13(1):2727. doi:

Insert

HDLBP (HDLBP Human)

Expression

Mammalian

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-v2 Puro EIF2AK3/PERK_sgRNA

Plasmid

#218666

Purpose

sgRNA targeting human EIF2AK3/PERK

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

EIF2AK3 (EIF2AK3 Human)

Use

CRISPR and Lentiviral

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-LAMP2

Plasmid

#222321

Purpose

Synchronize the trafficking of LAMP2 from the ER.

Depositor

Franck Perez

Article

Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

Insert

Streptavidin-KDEL and LAMP2 fused to SBP-EGFP (LAMP2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1280

Plasmid

#235712

Purpose

Expression of FL human VPS34-EGFP in mammalian cells

Depositor

Roger Williams

Article

Tremel et al Nat Commun. 2021 Mar 10;12(1):1564. doi:

Insert

VPS34 (PIK3C3 Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pME-EGFP-P2A-iCre (JDW 921)

Plasmid

#224519

Purpose

A Gateway compatible middle entry clone containing EGFP P2A iCre (codon optimized cre)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EGFP-P2A-iCre

Use

Cre/Lox

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-muscle Amphiphysin 2

Plasmid

#22213

Depositor

Pietro De Camilli

Article

Butler et al J Cell Biol. 1997 Jun 16. 137(6):1355-67

Insert

Amphiphysin II (BIN1 Human)

Tags

GFP

Expression

Bacterial and Mammalian

Available Since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pPICZ A-Strep-Opa1 (SB251)

Plasmid

#227600

Purpose

Inducible expression of Strep-tagged Opa1 in Pichia pastoris

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

OPA1 (OPA1 Human)

Tags

Twin-StrepTag

Expression

Yeast

Mutation

contains aa 253-960 only

Promoter

AOX1

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-hATG2A

Plasmid

#114462

Purpose

Expresses human ATG2A with GFP in mammalian cells

Depositor

Noboru Mizushima

Article

Tamura et al FEBS Lett. 2017 Dec;591(23):3819-3830. d

Insert

human ATG2A (ATG2A Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

P656R (please see depositors comment below)

Promoter

CMV

Available Since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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siRNA-resistant SigmaR1-mNeonGreen a5mut (F196A L199A F200A L203A Y206A L210A L214A Y217A)

Plasmid

#226578

Purpose

Encodes mutant siRNA resistant SigmaR1 protein (substitutions) labeled with mNeonGreen

Depositor

Gia Voeltz

Article

Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

Insert

SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)

Tags

mNeonGreen

Expression

Mammalian

Mutation

F196A, L199A, F200A, L203A, Y206A, L210A, L214A, …

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pET41b+_Npl4

Plasmid

#117108

Purpose

Production of recombinant Npl4 in E.coli

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

Npl4 (NPLOC4 Human)

Expression

Bacterial

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-SIN-BX SpyCatcher003-hCD52

Plasmid

#210565

Purpose

Expresses Spycatcher003 on the cell surface using human CD52 hinge

Depositor

Omer Dushek

Article

Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

Insert

Spycatcher003 fused to human CD52 hinge

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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CRY2-mCherry-Miro1TM

Plasmid

#102247

Purpose

Expresses fusion of CRY2PHR with mCherry and transmembrane domain of Miro1

Depositor

Bianxiao Cui

Article

Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

Insert

CRY2PHR-mCherry-Miro1TM (RHOT1, CRY2 Human, Mustard Weed)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pME-DreERT2 (JDW 1344)

Plasmid

#224523

Purpose

A Gateway-compatible middle entry clone containing Dre recombinase fused to mutant ERT2 for tamoxifen-inducible recombinase activity

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

DreERT2

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-ACTB

Plasmid

#207748

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of ACTB for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting N-terminus of ACTB (ACTB Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Solo-mStayGold-CETN2

Plasmid

#227292

Purpose

Donor template for mStayGold insertion into the N-terminus of the CETN2 locus. For centriole visualization. To be co-transfected with sgRNA plasmid px330-CETN2 (Addgene #227291)

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

CETN2 Homology Arms flanking a mStayGold Tag (CETN2 Human)

Use

CRISPR; Donor template

Expression

Mammalian

Promoter

Promoterless

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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GLuc-PM

Plasmid

#164783

Purpose

Gaussia luciferase (GLucM23) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptors

Depositor

Laszlo Hunyady

Article

Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.

Insert

Gaussia luciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta (PDGFRB Human, Gaussia princeps)

Tags

GLuc

Expression

Mammalian

Promoter

CMV

Available Since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

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