We narrowed to 24,953 results for: ina
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Plasmid#216531PurposeTargeting vector for CRISPRd generationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertABI-dCas9-mCherry
UseCRISPRTagsmCherryExpressionMammalianPromoterTRE3GAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8m-p10
Plasmid#218863PurposeLexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertRSET-jGCaMP8m
Tags6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…ExpressionInsectMutationA25G F286YPromoterhsp70Available sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8s-p10
Plasmid#218864PurposeGal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertRSET-jGCaMP8s
Tags6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…ExpressionInsectMutationS26M F286Y Q315HPromoterhsp70Available sinceMay 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
LLP783_SpyCatcher-DNMT3A-CO
Plasmid#211776PurposeSpyTag counterpart binding domain, SpyCatcher, fused to DNA methyltransferase DNMT3A, with GFP selectionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTraptavudin-DNMT3A
Tags3xV5ExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect DNMT3A…PromoterpEF1a and pSV40Available sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRM053 His-TEV-Cas9 triNLS 0C
Plasmid#196244PurposeExpression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)DepositorInsertCas9
UseCRISPRTagsHA, His6 (N terminal on backbone), NLS, Nucleopla…ExpressionBacterialMutationCys at positions 80 and 574 have been mutated to …Available sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKGmDUET-NgBR/hCIT
Plasmid#203173PurposeExpression of human cis-prenyltransferase complex for purificationDepositorTagsStrep-Tag II-TEV and internally 6HIS taggedExpressionMammalianPromoterCMVAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
LPUtopia-7
Plasmid#199212PurposeLanding Pad Utopia_v7 donor plasmid for AAVS1 site-specific integration in human cells. Use NeoR and eGFP as positive selection marker for AAVS1-targeted insertion and RFP as negative selection markerDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsaminoglycoside phosphotransferase,
enhanced Green Fluorescence Protein
Red Fluorescence Protein
ExpressionMammalianPromoterCMV and thymidine kinase promoterAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-3*Flag-APEX2 C-term EWSR1
Plasmid#187586PurposeExpress FLAG epitope and APEX2-tagged EWSR1 fusion protein in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEWSR1 (EWSR1 Human)
TagsAPEX2-FLAGExpressionMammalianPromoterCMVAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
NLS-4LNDD-pcDNA3.1(+)
Plasmid#172313PurposeExpresses ADAR2 Deaminase Domain (DD) fused with 4 repeats of lambda N peptide (4LN) with a Nuclear Localization Signal (NLS) in mammalian cellsDepositorInsertLambda N peptide - human ADAR2 Deaminase Domain
Tags6xHis, Flag, and Nuclear Localization Signal of S…ExpressionMammalianPromoterCMVAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-RP-CoV2/stSpike-puro
Plasmid#161794PurposeTransposon-based SARS-CoV-2 secretable trimeric Spike stable expression vector for protein productionDepositorInsertSARS-CoV-2 secretable trimeric Spike
UseTransposonTags6xHis and dTomatoExpressionMammalianPromoterEF1a/RPBSAAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr2-Gnaq
Plasmid#170298PurposeEncoding mouse GnaqDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertA recombinant mouse Gnaq (Gnaq Mouse)
TagsN/AExpressionMammalianMutationAsilent mutation was introduced into the sgRNA-ta…Available sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBa.Halo-myosin Va tail 1006-1828
Plasmid#162715PurposeMammalian expression of rat WT Myosin Va with deletion of the 1005 N-terminal residuesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMyosin Va (Myo5a Rat)
TagsHaloExpressionMammalianMutationWT with deletion of the 1005 N-terminal residues.PromoterChicken Beta actinAvailable sinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8541 PB-PGK-RR12EE345L-C term (406) dLbCpf1-2XNLS-miniVPR-mCherry-pA
Plasmid#140222PurposeConstitutive PGK driven expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to miniVPRDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertzipper with dLbCpf1 (Cas12a) C terminal half (406) fused to miniVPR
TagsmCherryExpressionMammalianMutationD832APromoterPGKAvailable sinceMay 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XHA-Src-KD
Plasmid#140312PurposeExpresses HA-tagged human Src-KD in mammalian cells for structure-function studiesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSrc mutant (SRC Human)
Tags2X HAExpressionMammalianMutationContains only the KD regionPromoterCMVAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRC/CMV-TYK2K930R-VSV
Plasmid#139346PurposeExpression of kinase-dead TYK2-K930R proteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
TagsVSVExpressionMammalianMutationV362F-K930RPromoterCMVAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
SOD1-VC
Plasmid#89758Purposeexpresses human SOD1 fused to the C-terminal part of VenusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSOD1 (SOD1 Human)
TagsC-terminal part of VenusExpressionMammalianPromoterCMVAvailable sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTE2690
Plasmid#137717PurposeExpresses dimpLbCas12a-SunTag in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdimpLbCas12a
UseCRISPRTagsNLSx3 SV40 and SunTagx10ExpressionMammalianMutationD156R, G532R, K538V, Y542R, K595R, D832A,PromoterCMVAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF17 tail 400-1038
Plasmid#134617PurposeMammalian expression of mouse KIF1A 400-1038DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertKIF17 (Kif17 Mouse)
TagseGFPExpressionMammalianMutationWT with deletion of the 399 N-terminal residues.PromoterChicken Beta actinAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDM304_DPAKa(GBD)-YFP
Plasmid#128699Purposeexpression of YFP-tagged probe for active Rac1 in Dictyostelium discoideum cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGBD domain (aa 731-890) of DPAKa
UseDictyostelium discoideum expression vectorTagsYFPMutationnoneAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJEP325-pAAV-FullH1TO-SaCa9sgRNAi(CREB)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA
Plasmid#113702PurposeDox-inducible H1 driven SaCas9 gRNA expression cassette with a gRNA targeting CREB. Followed by an EFS driven GFP-KASH in a separate reading frame.DepositorInsertsSaCas9 gRNA Cassete
GFP
UseAAV, CRISPR, and Mouse TargetingTagsKASHAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only