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pSTAP-seq_fly-tj

Plasmid

#86386

Purpose

Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.

Depositor

Alexander Stark

Article

Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

Insert

D. melanogaster tj enhancer

Use

Stap-seq screening vector

Available Since

June 2, 2017

Availability

Academic Institutions and Nonprofits only

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pAG180 SGCD 3'UTR mut

Plasmid

#12038

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N13 3'UTR mut (SGCD Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Available Since

June 8, 2006

Availability

Academic Institutions and Nonprofits only

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pAG222 PIK3CG 3'UTR mut

Plasmid

#12052

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N23 3'UTR mut (PIK3CG Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Available Since

June 8, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3 PARL-FLAG-CT S70A

Plasmid

#13620

Depositor

Luca Pellegrini

Article

Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

Insert

Presenilin-associated rhomboid-like (PARL, PARL Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 70 to Ala

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
hSTARR-seq_SULT1C vector

Plasmid

#99294

Purpose

Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

Depositor

Alexander Stark

Article

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Type

Empty backbone

Use

Hstarr-seq screening vector

Expression

Mammalian

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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hSTARR-seq_TTF2 vector

Plasmid

#99293

Purpose

Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

Depositor

Alexander Stark

Article

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Type

Empty backbone

Use

Hstarr-seq screening vector

Expression

Mammalian

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf110

Plasmid

#12798

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf110

Expression

Mammalian

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pCfB2991

Plasmid

#63643

Purpose

pCfB2991 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Promoter

none

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pMX Vav1 Y3F/Grb2bindb* (P657A) uGFP

Plasmid

#14563

Depositor

Joan Brugge

Article

Wilsbacher et al Cell Commun Signal. 2006 May 18;4:5. doi

Insert

Vav1 (VAV1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Y3F/Grb2bindb* (P657A)

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
pET15b+PCNA3 R112C/T180C

Plasmid

#66182

Purpose

Expresses the R112C/T180C mutant of Sulfolobus solfataricus PCNA3

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 3

Tags

His6 tag

Expression

Bacterial

Mutation

R112C, T180C

Promoter

T7 promoter

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pET15b+PCNA2 E105C/L171C

Plasmid

#66178

Purpose

Expresses the E105C/L171C mutant of Sulfolobus solfataricus PCNA2

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 2

Tags

His6 tag

Expression

Bacterial

Mutation

E105C, L171C

Promoter

T7 promoter

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pAG76 GLCCI1 3'UTR

Plasmid

#12029

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

GLCCI1 3'UTR (GLCCI1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only

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MSCV-mouse-MycNV419D-IRES-RFP

Plasmid

#73574

Purpose

Mammalian expression of mouse MycN V419D mutant

Depositor

Martine Roussel

Article

Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

Insert

Mycn (Mycn Mouse)

Use

Retroviral

Tags

IRES-RFP

Expression

Mammalian

Mutation

V419D

Promoter

MSCV

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pmalc4x_V426F_TEV

Plasmid

#47702

Purpose

Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation V426F

Depositor

Raquel Lieberman

Article

Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.

Insert

Olfactomedin-like domain with V426F mutation (MYOC Human)

Tags

MBP

Expression

Bacterial

Mutation

changed V426F

Promoter

Ptac

Available Since

Sept. 9, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pBS BRD7

Plasmid

#19232

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Bromodomain containing 7 (BRD7 Chicken)

Expression

Bacterial

Available Since

Oct. 8, 2008

Availability

Academic Institutions and Nonprofits only
pBS TMEM108

Plasmid

#18852

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Hypothetical protein, Tmem108 (HAPLN1 Chicken)

Expression

Bacterial

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
pDESTFLAGHApuro3-Ago2K570A

Plasmid

#14102

Depositor

Thomas Tuschl

Article

Ma et al Nature. 2005 Mar 31. 434(7033):666-70.

Insert

Ago2 (AGO2 Human)

Tags

FLAG/HA

Expression

Mammalian

Mutation

changed Lys570 to Ala

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
Tmem194 pEGFP-N2 (1074)

Plasmid

#62027

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

Tmem194 (TMEM194A Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pAAV-Ef1a-DIO-PBG-WPRE-hGH

Plasmid

#74287

Purpose

pAAV vector to express PBG (chimeric Glycoprotein) in a Cre-dependent manner

Depositor

Edward Callaway

Article

Kim et al Neuron. 2015 Dec 16;88(6):1253-67. doi:

Insert

PBG (chimeric rabies Glycoprotein)

Use

AAV

Promoter

Ef1a

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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pICSL80037

Plasmid

#68260

Purpose

Level 0 Golden Gate Part: Coding sequence, neomycin phophotransferase II (Escherichia coli)

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Coding sequence, neomycin phophotransferase II (Escherichia coli)

Use

Synthetic Biology

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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