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pREP3-TEV-NLS

Plasmid

#21146

Depositor

Stephen Kearsey

Publication

Yang et al BMC Mol Biol. 2005 Jun 7;6:13. doi: 10.1

Insert

TEV protease (TEVgp1 tobacco etch virus)

Use

S. pombe

Tags

2xNLS, NLS, and myc

Expression

Yeast

Available since

July 6, 2009

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-VAPA(1-242) K87D/M89D

Plasmid

#18875

Depositor

Axel Brunger

Publication

Kaiser et al Structure. 2005 Jul . 13(7):1035-45.

Insert

VAP-A (Vapa Rat)

Tags

GFP

Expression

Mammalian

Mutation

double point mutant K87D / M89D

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
FLAG-MED4

Plasmid

#15425

Depositor

Joan Conaway, Ronald Conaway

Publication

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED4 (MED4 Human)

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pMX Vav1 Y3F uGFP

Plasmid

#14558

Depositor

Joan Brugge

Publication

Wilsbacher et al Cell Commun Signal. 2006 May 18;4:5. doi

Insert

Vav1 (VAV1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Y3F (Y142, Y160, Y174) in DH domain

Available since

April 12, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pJ3 SHP2 E76A

Plasmid

#8321

Depositor

Ben Neel

Publication

Bennett et al Mol Cell Biol 1996 Mar;16(3):1189-202.

Insert

SHP2 (PTPN11 Human)

Expression

Mammalian

Mutation

E76A (activated mutant)

Available since

June 17, 2005

Availability

Academic Institutions and Nonprofits only

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pcDNA3-endo-EKAR4

Plasmid

#205513

Purpose

Genetically encoded FRET-based biosensor for monitoring ERK kinase activity. Targeted to endosomes.

Depositor

Jin Zhang

Publication

Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

Insert

endo-EKAR4

Tags

2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and 6xHI…

Expression

Mammalian

Promoter

CMV

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBITE-EGFRvIII-F269-OKT3

Plasmid

#190681

Purpose

Encodes an EGFRvIII-CD3 BITE protein (F269 clone)

Depositor

Scott McComb

Publication

Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

Insert

Anti-EGFRvIII-scFv-F269-modularized-OKT3-HisTag

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Promoter

EFS

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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METTL3 shRNA 2 tet pLKO puro

Plasmid

#162984

Purpose

Tet-inducible shRNA targeting human METTL3 #2

Depositor

Mark Rubin

Publication

Cotter et al Mol Cancer Res. 2021 Jun 4. pii: 1541-77

Insert

methyltransferase like 3 (METTL3 Human)

Use

Lentiviral

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP_sfCherry2(11)_Clathrin light chain

Plasmid

#83032

Purpose

Expresses sfCherry2(11) tagged clathrin light chain in mammalian cells

Depositor

Bo Huang

Publication

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

sfCherry2(11)_Clathrin light chain (CLTA Synthetic, Human)

Expression

Mammalian

Promoter

CMV

Available since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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pAc5 HA3-eDHFR-T2A-puro

Plasmid

#86395

Purpose

for C-terminal tagging with eDHFR trimethoprim regulated degron with puro selection

Depositor

David Bentley

Publication

Sheridan et al Biotechniques. 2016 Feb 1;60(2):69-74. d

Insert

HA3 eDHFR T2A puro

Use

CRISPR

Tags

HA3 eDHFR T2A puro

Mutation

R12Y, G67S and Y100I mutations in E coli DHFR

Available since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GFP-p38beta

Plasmid

#86833

Purpose

mammalian expression of GFP-p38beta

Depositor

Rony Seger

Publication

Zehorai et al Mol Cell Biol. 2014 Jan;34(2):259-70. do

Insert

p38beta (MAPK11 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) Drp1 V2 S600A

Plasmid

#46344

Depositor

David Chan

Publication

Loson et al Mol Biol Cell. 2013 Jan 2.

Insert

Drp1 V2 (Dnm1l Mouse)

Expression

Mammalian

Mutation

S600A

Promoter

CMV

Available since

July 23, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
CMV/N3IC-HA

Plasmid

#47618

Purpose

in vitro transcription and translation of HA-tagged Notch3 Intracellular domain

Depositor

Urban Lendahl

Publication

Lardelli et al Mech Dev. 1996 Oct;59(2):177-90.

Insert

Notch3 Intracellular domain (Notch3 Mouse)

Use

In vitro transcription and translation

Tags

HA

Expression

Mammalian

Mutation

codons 1664 (Met) to 2318 (Ala)

Promoter

CMV

Available since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
2XMyc-LRRK2-WD40

Plasmid

#25073

Depositor

Mark Cookson

Publication

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Tags

2XMyc

Expression

Mammalian

Mutation

Contains only the WD40 domain aa 2155-2527

Available since

Nov. 1, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pAdv/TERT

Plasmid

#26744

Depositor

Bingliang Fang

Publication

Gu et al Cancer Res. 2000 Oct 1. 60(19):5359-64.

Insert

human TERT promoter (TERT Human)

Expression

Mammalian

Available since

March 15, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV-Flag YAP127/128/131/381A

Plasmid

#27378

Depositor

Kunliang Guan

Publication

Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

Insert

YAP (YAP1 Human)

Tags

2xFLAG

Expression

Mammalian

Mutation

S127/128/131/381A

Available since

March 4, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3.1 FLIPglu-3.2mDelta13V

Plasmid

#18011

Depositor

Wolf Frommer

Publication

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

FLIPglu F16A S112A Delta13

Tags

ECFP and Venus

Expression

Mammalian

Mutation

F16A, S112A in binding protein

Available since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pLKO-1 shRev-Erbalpha

Plasmid

#22747

Depositor

Bruce Spiegelman

Publication

Estall et al Proc Natl Acad Sci U S A. 2009 Dec 29. 1

Insert

shRev-Erbalpha (Nr1d1 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

Feb. 8, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pRS1422

Plasmid

#12548

Depositor

Robert Sobol

Publication

Trivedi et al Cancer Res. 2005 Jul 15. 65(14):6394-400

Insert

human Methylpurine DNA glycosylase (MPG Human)

Expression

Mammalian

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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PRDM9(KRAB-SSXRD-PR/SET-ZF1-dC)-Cas9

Plasmid

#184464

Purpose

Plasmid encoding PRDM9-Cas9 fusion under CMV promoter

Depositor

Jennifer Doudna

Publication

Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

Insert

PRDM9(KRAB-SSXRD-PR/SET-ZF1-dC)-Cas9 (PRDM9 Human)

Use

CRISPR

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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