We narrowed to 11,647 results for: emb
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Plasmid#148954PurposeMammalian Expression of HsGIGYF2-W288AF300AF306ADepositorInsertHsGIGYF2-W288AF300AF306A (GIGYF2 Human)
ExpressionMammalianMutationone silent mutation and one deletion of Q1211 com…Available sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsGIGYF2_1-718_AI
Plasmid#148948PurposeMammalian Expression of HsGIGYF2_1-718DepositorInsertHsGIGYF2_1-718 (GIGYF2 Human)
ExpressionMammalianMutationone silent mutation and one deletion of Q1211 com…Available sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsGIGYF2_280-310_AI
Plasmid#148951PurposeMammalian Expression of HsGIGYF2_280-310DepositorInsertHsGIGYF2_280-310 (GIGYF2 Human)
ExpressionMammalianMutationone silent mutation and one deletion of Q1211 com…Available sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsGIGYF2-F300AF306A_AI
Plasmid#148952PurposeMammalian Expression of HsGIGYF2-F300AF306ADepositorInsertHsGIGYF2-F300AF306A (GIGYF2 Human)
ExpressionMammalianMutationone silent mutation and one deletion of Q1211 com…Available sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::B1-NLSLacZ-B2
Plasmid#186413PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined NLSLacZ under control of an AttB3/B5 recombined reg. sequence.DepositorPublicationAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-EGFP-eDHFR(69K6)-cRaf
Plasmid#178849PurposeMammalian expression of cRaf fused to EGFP-eDHFR(69K6)DepositorAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-cRaf-mNG-eDHFR(69K6)
Plasmid#172107PurposeMammalian expression of cRaf fused to mNeonGreen-eDHFR(69K6)DepositorAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX2T-TEV CHMP1B 4-199 L188A/L192A
Plasmid#108289PurposeExpresses residues 4-199 with L to A mutations at residues 188 and 192 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-284DepositorInsertCHMP1B (CHMP1B Human)
TagsGSTExpressionBacterialMutationchanged L 188 to A, changed L 192 to AAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX2T-TEV CHMP1B 4-199 L192A/L195A
Plasmid#108291PurposeExpresses residues 4-199 with L to A mutations at residues 192 and 195 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-285DepositorInsertCHMP1B (CHMP1B Human)
TagsGSTExpressionBacterialMutationchanged L 192 to A, changed L 195 to AAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot1iso2-del1844-2133_T
Plasmid#147628PurposeMammalian Expression of HsNot1iso2-del1844-2133DepositorInsertHsNot1iso2-del1844-2133 (CNOT1 Human)
ExpressionMammalianMutation4 silent and two non silent G917E and R2353Q muta…Available sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HsNot3-del607-634-F673E_S
Plasmid#147489PurposeMammalian Expression of HsNot3-del607-634-F673EDepositorInsertHsNot3-del607-634-F673E (CNOT3 Human)
ExpressionMammalianMutationtwo silent mutations compared to the sequence giv…Available sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-gamma-tubulin(N229A)-TEV-His6
Plasmid#178077PurposeInsect cell expression of gamma-tubulin(N229A)-TEV-His6DepositorInsertgamma-tubulin with an N229A point mutation (TUBG1 Human)
TagsTEV-His6ExpressionInsectMutationChanged Asparagine 229 to AlanineAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV Tmx4-V5 C209A
Plasmid#175140PurposeLentiviral expression of V5-tagged mouse Tmx4DepositorAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV Tmx4-V5 C211A
Plasmid#175139PurposeLentiviral expression of V5-tagged mouse Tmx4DepositorAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-uTEV1-MAVS-mTA3
Plasmid#171001PurposeHiLITR protease with MAVS [Y536delinsFIV] C-terminal tmd targeting information (Mitochondria and ER insertion)DepositorInsertEGFP-uTEV1-MAVS(tmd)[Y536delinsFIV]
UseLentiviral and Synthetic BiologyExpressionMammalianMutationMAVS(tmd)[Y536delinsFIV]PromoterpTRE-tight (TetOn)Available sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH_CMV_L6_mKate2
Plasmid#167332PurposeExpresses mKate2 fused to TFAM L6 mutant in mammalian cellsDepositorInsertTFAM (TFAM Human)
UseLentiviralTagsmKate2ExpressionMammalianMutationChanged K136, H137, K139, R140, K146 and K147 to …PromoterCMVAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS405 VAC8-C4A,C5A,C7A-GFP
Plasmid#166838PurposeExpresses a palmitoylation mutant Vac8-GFP in yeasts, mutations are Cys4Ala, Cys5Ala, Cys7AlaDepositorInsertVAC8 (VAC8 Budding Yeast)
TagsGFPExpressionYeastMutationC4A, C5A, C7APromoterVAC8 promoterAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-optimizedNedd4.2F823D
Plasmid#165105Purposebacterial expression of a GST fusion of Human Nedd4.2d F823DDepositorInsertNedd4.2d F823D (NEDD4L Human)
TagsGST from plasmid.ExpressionBacterialMutationcodon optimized for E. coli expression; F823D; …Available sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 sgRNA sCRNA 2.0 (GB2461)
Plasmid#160593PurposeVersion of the native Cas9-sgRNA with one native WT aptamer sequence and F6 aptamer sequence recognized for Ms2 coat protein, in 3'.DepositorInsertsgRNA sCRNA 2.0
UseCRISPRMutationBsaI and BsmBI sites removedAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDUAL_2_lacZ
Plasmid#155173Purposesee pDUAL_lacZ: exchanged origin of replication (pBR322) and introduced bom siteDepositorInsertsSucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda
UseSynthetic BiologyTagsHis Tag and Strep - Tag IIExpressionBacterialAvailable sinceOct. 9, 2020AvailabilityAcademic Institutions and Nonprofits only