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pTETRIS-cargo

Plasmid

#126033

Purpose

Luciferase reporter construct for the study of cis-repressive activity in long noncoding RNAs. Also confers puromycin resistance.

Depositor

Mauro Calabrese

Publication

Kirk et al Nat Genet. 2018 Oct;50(10):1474-1482. do

Type

Empty backbone

Use

Luciferase; Piggybac

Expression

Mammalian

Promoter

TRE

Available since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHAGE-RAC1

Plasmid

#116783

Purpose

Lentiviral expression of RAC1

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

RAC1 (RAC1 Human)

Use

Lentiviral

Mutation

WT

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pMRE137

Plasmid

#118491

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mClover3

Expression

Bacterial

Available since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCLX-M38s/TOP-dGFP

Plasmid

#114295

Purpose

Expression of destabilized GFP under the control of the M38-TOP canonical Wnt signaling responsive promoter

Depositor

Patrick Salmon

Publication

Boitard et al Cell Rep. 2015 Mar 3;10(8):1349-61. doi:

Insert

dGFP

Use

Lentiviral

Mutation

destabilized GFP

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Chronos-mRuby2-ST

Plasmid

#105446

Purpose

Cation channelrhodopsin Chronos fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Publication

Pegard et al Nat Commun. 2017 Oct 31;8(1):1228. doi:

Insert

Chronos-mRuby2-ST

Tags

mRuby2 and soma targeting motif

Promoter

pCAGGS

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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5LTR-puro-3LTR

Plasmid

#86948

Purpose

PiggyBac transposon

Depositor

Rob Mitra

Publication

Qi et al Nucleic Acids Res. 2017 Jan 12. pii: gkw

Insert

PiggyBac transposon

Expression

Mammalian

Available since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pUBC_HA_L7Ae-VP64

Plasmid

#68421

Purpose

Transient expression of humanized A. fulgidus L7Ae, fused to the VP64 transcription activator, in mammalian cells, under a UBC promoter

Depositor

John Rinn

Publication

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

L7Ae

Use

Synthetic Biology

Tags

HA and VP64

Expression

Mammalian

Promoter

human UbC

Available since

Sept. 21, 2015

Availability

Academic Institutions and Nonprofits only

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p3E-mVenus

Plasmid

#67719

Purpose

Multisite gateway entry clone for adding C-terminal fusions of mVenus (no ATG)

Depositor

Rob Parton

Publication

Morsch et al Front Cell Neurosci. 2015 Aug 31;9:321.

Insert

mVenus

Use

Gateway multisite 3' entry clone

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJZC593

Plasmid

#62319

Purpose

sgRNA with MS2-PP7 for yeast cells

Depositor

Wendell Lim, Stanley Qi

Publication

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA + MS2-PP7 RNA binding module

Expression

Yeast

Promoter

SNR52

Available since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GLRX2

Plasmid

#38795

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

GLRX2 (Glrx2 Human)

Tags

His Tev

Expression

Bacterial

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pGL3Basic-Mdm2-T3

Plasmid

#32369

Depositor

Hong Wu

Publication

Chang et al J Biol Chem. 2004 Jul 9;279(28):29841-8.

Insert

Mdm2 promoter (Mdm2 Mouse)

Expression

Mammalian

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pLminP_Luc2P_RE54

Plasmid

#90399

Purpose

C/EBPa - CEBPA gene reporter

Depositor

Ramnik Xavier

Publication

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

CEBPa

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE50

Plasmid

#90389

Purpose

PPARg/RARa - PPARg gene reporter

Depositor

Ramnik Xavier

Publication

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

ACO1

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE15

Plasmid

#90354

Purpose

RARA - Retinoic acid receptor gene reporter

Depositor

Ramnik Xavier

Publication

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

RARE

Available since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE14

Plasmid

#90349

Purpose

VDR - Vitamin D receptor gene reporter

Depositor

Ramnik Xavier

Publication

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

VDR

Available since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBacMam2_N_His8_eGFP_TEV

Plasmid

#178806

Purpose

Baculovirus gene transfer into Mammalian cells to product recombinant proteins

Depositor

Kazuhiro Abe

Type

Empty backbone

Tags

His8, eGFP, TEV

Expression

Mammalian

Promoter

CMV

Available since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pYC1446

Plasmid

#158720

Purpose

Integrating plasmid expressing Sth1 Cas9 and the cognate sgRNA

Depositor

Yi-Cheng Sun

Publication

Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

Insert

Sth1 sgRNA scaffold

Use

CRISPR

Expression

Bacterial

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR E3 pR8A L3-L2

Plasmid

#158735

Purpose

Plant mitoTALEN vector assembly, Entry clone 3 with attL3 L2 (last NN)

Depositor

Shin-ichi Arimura

Publication

Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

Insert

TALEN ORF without TAL repeat

Use

TALEN

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pENTR E1 pF8A L1-L4

Plasmid

#158731

Purpose

Plant mitoTALEN vector assembly, Entry clone 1 with attR4 R3 (last NN)

Depositor

Shin-ichi Arimura

Publication

Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

Insert

TALEN ORF without TAL repeat

Use

TALEN

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pENTR E1 pF7A L1-L4

Plasmid

#158730

Purpose

Plant mitoTALEN vector assembly, Entry clone 1 with attR4 R3 (last NI)

Depositor

Shin-ichi Arimura

Publication

Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

Insert

TALEN ORF without TAL repeat

Use

TALEN

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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