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pENTR-SYNZIP13

Plasmid

#80668

Purpose

Encodes the synthetic coiled coil peptide SYNZIP13

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP13

Use

Gateway Cloning

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR-SYNZIP14

Plasmid

#80669

Purpose

Encodes the synthetic coiled coil peptide SYNZIP14

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP14

Use

Gateway Cloning

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR-SYNZIP10

Plasmid

#80665

Purpose

Encodes the synthetic coiled coil peptide SYNZIP10

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP10

Use

Gateway Cloning

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCsC_spacer

Plasmid

#136077

Purpose

Use when making odd level constructs (L3,...) if pCsC position (position 3) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Even spacer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCsE_spacer

Plasmid

#136078

Purpose

Use when making odd level constructs (L3,...) if pCsE position (position 4) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Even spacer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CDS12_mScarlet-I

Plasmid

#136087

Purpose

FP for N-term fusion with a CTAG

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CDS12_mScarletI(noStop)

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCk1_spacer

Plasmid

#136071

Purpose

Use when making even level constructs (L2,...) if pCk1 position (position 1) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Odd spacer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pRD404

Plasmid

#163109

Purpose

Expression of mNeonGreen_H-NSdbd in Escherichia coli (and, potentially, other bacteria)

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

mNeonGreen_H-NSdbd

Expression

Bacterial

Promoter

araBAD promoter

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pRD395

Plasmid

#163102

Purpose

Expression of mCherry_H-NSdbd in Escherichia coli (and, potentially, other bacteria)

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

mCherry_H-NSdbd

Expression

Bacterial

Promoter

araBAD promoter

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pLBS.EF NLS-QUE37C

Plasmid

#129325

Purpose

ATP measurement, nucleus, lentivirus

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN-37C

Use

Lentiviral

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pJS699

Plasmid

#168779

Purpose

Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) and can be combined with pJS697 through homologous recombination

Depositor

Timothy Whitehead

Article

Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

Insert

Spike RBD (S betacoronavirus)

Tags

Cmyc

Mutation

N343Q

Promoter

GAL1

Available Since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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JGI_018319

Plasmid

#158837

Purpose

Rare earth element alcohol dehydrogenases

Depositor

Joint Genome Institute

Article

Huang et al ISME J. 2019 Aug;13(8):2005-2017. doi: 1

Insert

Mex_1740

Use

Synthetic Biology

Available Since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pTYF-super-mfPENK-2.2k-Venus-WPRE

Plasmid

#156400

Purpose

Expresses Venus under PENK gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pME-myristo

Plasmid

#109541

Purpose

Multisite gateway vector for 5' tagging with the 5' myristoylation sequence from ARF5

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

5' myristoylation sequence from ARF5

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pJOE4847.2

Plasmid

#135086

Purpose

rhamnose-inducible expression vector

Depositor

Josef Altenbuchner

Article

Motejadded et al Biotechnol Lett. 2009 Apr;31(4):543-9. d

Insert

MalE-6xHis-Ulp1

Expression

Bacterial

Available Since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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JPF0419v

Plasmid

#124021

Purpose

Encodes pCAG driving expression of multicistronic Lyn-tagged iRFP713, cytoplasmic mAzamiGreen, mCerulean-tethered p38 KTR, and Histone 2B fused to mScarlet in a PiggyBac destination vector

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB_pCAG-Lyn-iRFP713_pCAG-NES-mAzamiGreen_pCAG-p38KTR-mCerulean_pCAG-H2B::mScarlet

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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JPF0522

Plasmid

#124013

Purpose

Encodes crippled pEF1a driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB_pEF1ac-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pMOD3-mWasabi-Sh_ble

Plasmid

#120332

Purpose

Bacterial mWasabi promoter trap

Depositor

James Kirby

Article

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

mWasabi

Expression

Bacterial

Promoter

(None)

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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T7-WbpB-His6

Plasmid

#89711

Purpose

Expresses WbpB from P. aeruginosa with an N-terminal T7 tag and a C-terminal His6 tag

Depositor

Barbara Imperiali

Article

Zamora et al Methods Enzymol. 2017;597:145-186. doi:

Insert

WbpB (wbpB Pseudomonas aeruginosa)

Tags

His6 and T7

Expression

Bacterial

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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T7-WbpE-His6

Plasmid

#89712

Purpose

Expresses WbpE from P. aeruginosa with an N-terminal T7 tag and a C-terminal His6 tag

Depositor

Barbara Imperiali

Article

Zamora et al Methods Enzymol. 2017;597:145-186. doi:

Insert

WbpE (wbpE Pseudomonas aeruginosa)

Tags

His6 and T7

Expression

Bacterial

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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