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pEntry_MERS-CoV-nsp16

Plasmid

#168826

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

MERS-CoV-nsp16 (orf1ab )

Use

Gateway-compatible entry vector

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-hPLEKHA6-Myc

Plasmid

#236540

Purpose

For expression of PLEKHA6 (human) in mammalian cells

Depositor

Sandra Citi

Article

Sluysmans et al Mol Biol Cell. 2021 Nov 1;32(21):ar34. d

Insert

PLEKHA6 (PLEKHA6 Human)

Tags

GFP and Myc

Expression

Mammalian

Mutation

WT

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pMDC32B-B/c

Plasmid

#227963

Purpose

Expression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

Depositor

Alberto Carbonell

Article

Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Insert

B/c

Expression

Plant

Promoter

35S

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6-CFAPoptim-KAN

Plasmid

#221128

Purpose

Plasmid for generating integration cassette to C-terminally tag gene of interest with FAPoptim::KANMX

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

FAPoptim

Expression

Yeast

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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pRS415-TEF1pr-SEC63-FAPoptim

Plasmid

#221116

Purpose

FAP tagged SEC63 (C-terminally tagged, optimized) under the Tef1 promoter with 2xMYC tag

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

FAPoptim-SEC63

Expression

Yeast

Promoter

TEF1

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRS415-TEF1pr-SEC61-FAPoptim

Plasmid

#221115

Purpose

FAP tagged SEC61 (C-terminally tagged, optimized) under the Tef1 promoter with 2xMYC tag

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

SEC61-FAPoptim

Expression

Yeast

Promoter

TEF1

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pYI2206

Plasmid

#228348

Purpose

Constitutive rPhlTA mutant expression

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

phlTA mutant

Use

Synthetic Biology

Tags

Three copies of VP16 (VP48)-Nuclear localization …

Expression

Yeast

Mutation

P5S, S6P, K86T, F109L, Q117R, E143K

Promoter

KpGAPDH promoter

Available Since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

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pMT478

Plasmid

#228286

Purpose

Constitutive mutant rPhlTA expression with strong promoter

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

rphlTA mutant

Use

Synthetic Biology

Tags

Three copies of VP16 (VP48)-Nuclear localization …

Expression

Yeast

Mutation

P5S, S6P, K86T, F109L, Q117R, E143K

Promoter

KpGAPDH promoter

Available Since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-ANP1-FAPoptim

Plasmid

#221120

Purpose

FAP-tagged marker for the cis-Golgi (FAP optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

ANP1-FAPoptim

Expression

Yeast

Promoter

TEF1

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-ERG6-FAPoptim

Plasmid

#221121

Purpose

FAP-tagged marker for lipid droplets (FAP optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

ERG6-FAPoptim

Expression

Yeast

Promoter

TEF1

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-PMA1-FAPoptim

Plasmid

#221122

Purpose

FAP-tagged marker for the plasma membrane (FAP optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

PMA1-FAPoptim

Expression

Yeast

Promoter

TEF1

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-SEC7-FAPoptim

Plasmid

#221124

Purpose

FAP-tagged marker for the trans-Golgi (FAP optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

SEC7-FAPoptim

Expression

Yeast

Promoter

TEF1

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-VPH1-FAPoptim

Plasmid

#221125

Purpose

FAP-tagged marker for the vacuolar membrane (FAP optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

VPH1-FAPoptim

Expression

Yeast

Promoter

TEF1

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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NOLC1 atgRNA pDY2250

Plasmid

#219860

Purpose

attachment site pegRNA for human NOLC1

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

Insert

NOLC1 atgRNA (NOLC1 Human)

Use

CRISPR

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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NOLC1 nicking guide pDY2251

Plasmid

#219861

Purpose

Nicking sgRNA for NOLC1

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

Insert

NOLC1 nicking sgRNA (NOLC1 Human)

Use

CRISPR

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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VirTREX2-HLDel-1_NbATML1-1pro

Plasmid

#231149

Purpose

T-DNA encoding TRV2 with TREX2 and mobile gRNA targeting NbATML1-1pro

Depositor

Daniel Voytas

Article

Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

Insert

TREX2 and mobile gRNA targeting NbATML1-1pro

Expression

Plant

Promoter

PEBV sub-genomic

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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VirTREX2-HLDel-1_NbSTM-5'UTR

Plasmid

#231148

Purpose

T-DNA encoding TRV2 with TREX2 and mobile gRNA targeting NbSTM-5?UTR

Depositor

Daniel Voytas

Article

Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

Insert

TREX2 and mobile gRNA targeting NbSTM-5?UTR

Expression

Plant

Promoter

PEBV sub-genomic

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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Const.6 EGFP-P2A-dTomato-T2A-TagBFP-IRES-Luc-PS9

Plasmid

#231910

Purpose

For packaging EGFP, dTomato, TagBFP and firefly luciferase mRNA into SARS-CoV-2 virus-like particles (VLPs)

Depositor

Sriram Neelamegham

Article

Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Insert

EGFP-P2A-dTomato-T2A-TagBFP-IRES-Luc

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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Const.5 EGFP-P2A-dTomato-IRES-Luc-PS9

Plasmid

#231909

Purpose

For packaging EGFP, dTomato and firefly luciferase mRNA into SARS-CoV-2 virus-like particles (VLPs)

Depositor

Sriram Neelamegham

Article

Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Insert

EGFP-P2A-dTomato-IRES-Luc

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pRExT2A-NT-BmNG

Plasmid

#232204

Purpose

Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: BSD-3xTy-T2A-3xTy-mNeonGreen

Depositor

Calvin Tiengwe

Article

Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

Insert

mNeongreen

Expression

Bacterial

Available Since

March 3, 2025

Availability

Academic Institutions and Nonprofits only

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