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FUG-T2A-Pten

Plasmid

#140738

Purpose

Lentiviral vector to overexpress human PTEN and GFP

Depositor

Bryan Luikart

Article

Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

Insert

GFP-PTA-PTEN (PTEN Human)

Use

Lentiviral

Tags

none

Expression

Mammalian

Promoter

hUbiC

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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MultiMate 5 colors

Plasmid

#206266

Purpose

Expression of 5 fluorescently labelled proteins in mammalian cells. Can be used as a CRE-donor (MultiBac system)

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

H2B (H. Sapiens), Actin and Tubulin (B. taurus), mito mCherry (Synthetic), CyOFP1 (E. quadricolor)

Use

Recombinant baculovirus production, cre acceptor…

Expression

Mammalian

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CD81-(TEV)-FKBP-Myc

Plasmid

#177908

Purpose

human CD81-TEV cleavage site-FKBP-Myc tag

Depositor

Masaharu Somiya

Article

Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

Insert

human CD81 (CD81 Human, Synthetic)

Tags

FKBP, Myc tag, and TEV protease cleavage site

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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JpExpress404 PTEN

Plasmid

#49420

Purpose

Bacterial Expression Vector

Depositor

Ramon Parsons

Article

Hopkins et al Science. 2013 Jul 26;341(6144):399-402.

Insert

PTEN (PTEN )

Expression

Bacterial

Promoter

T7

Available Since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only
G718A_BattB(GA)_TKterm_AttB(GT)_sGFP-PTEN-IRES-mCherry-P2A-HygroR_noTerm_attP(GA)_IRES-UnaG-2A-Puro-Term

Plasmid

#200634

Purpose

Plasmid self-excising recombination plasmid for Matreyek Bxb1(GT) landing pad

Depositor

Kenneth Matreyek

Article

Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

Insert

PTEN (PTEN Human)

Expression

Mammalian

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV FLIPi P2G_Thy1.1 Dbl (p53,PTEN)

Plasmid

#19746

Depositor

Richard Hynes

Article

Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

Insert

oligo targeting p53 and PTEN (Pten Mouse)

Use

Cre/Lox, RNAi, and Retroviral

Expression

Mammalian

Available Since

Dec. 18, 2008

Availability

Academic Institutions and Nonprofits only

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RB-409-815-pGex2T

Plasmid

#21784

Depositor

William Kaelin

Article

Kaelin et al Cell. 1991 Feb 8. 64(3):521-32.

Insert

Rb (RB1 Human)

Tags

GST

Expression

Bacterial

Mutation

deletion mutant ORF contains aa 409-815 of wt Rb …

Available Since

Aug. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pGEX-4T3-ZO-2-1st-PDZ-delta-NLS

Plasmid

#27425

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Tags

GST

Expression

Bacterial

Mutation

Deletion of NLS-Nuclear Localization Signal: Nucl…

Available Since

May 18, 2011

Availability

Academic Institutions and Nonprofits only
pCMV-myc-Sema4D RNAiR

Plasmid

#51600

Purpose

expresses RNAi-resistant mouse Sema4D with N-terminal myc tag

Depositor

Suzanne Paradis

Article

Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

Insert

Sema4D (Sema4d Mouse)

Tags

myc

Expression

Mammalian

Mutation

silent mutations between amino acids (approx 275-…

Promoter

CMV

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
pFLAG-GATA2-Ala(381-385)

Plasmid

#1423

Depositor

Gokhan Hotamisligil

Article

Tong et al Mol Cell Biol 2005 Jan;25(2):706-15.

Insert

GATA-2 (GATA2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

alanine substitutions at amino acids 381-385

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pFLAG-GATA2-Ala(388-394)

Plasmid

#1424

Depositor

Gokhan Hotamisligil

Article

Tong et al Mol Cell Biol 2005 Jan;25(2):706-15.

Insert

GATA-2 (GATA2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

alanine substitutions at amino acids 388-393; M39…

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pEGFPc1 PTEN C124S

Plasmid

#237340

Purpose

transient overexpression in mammalian cells

Depositor

Michele Pagano

Article

Kuchay et al Nature. 2017 Jun 22;546(7659):554-558. d

Insert

PTEN (PTEN Human)

Expression

Mammalian

Mutation

C124S

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV(MinDis)-iEscSnFR_PM

Plasmid

#182813

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iEscSnFR_PM

Expression

Mammalian

Promoter

hCMV

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV(MinDis)-iEscSnFR_cyto

Plasmid

#182814

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iEscSnFR_cyto

Expression

Mammalian

Promoter

hCMV

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV(MinDis)-iFluoxSnFR_ER

Plasmid

#182815

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iFluoxSnFR_ER

Expression

Mammalian

Promoter

hCMV

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV(MinDis)-iFluoxSnFR_PM

Plasmid

#182816

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iFluoxSnFR_PM

Expression

Mammalian

Promoter

hCMV

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV(MinDis)-iFluoxSnFR_cyto

Plasmid

#182817

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iFluoxSnFR_cyto

Expression

Mammalian

Promoter

hCMV

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV9-hSyn-iEscSnFR_ER

Plasmid

#182818

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iEscSnFR_ER

Use

AAV

Promoter

hSyn

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV9-hSyn-iEscSnFR_PM

Plasmid

#182819

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iEscSnFR_PM

Use

AAV

Promoter

hSyn

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV9-hSyn-iEscSnFR_cyto

Plasmid

#182820

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iEscSnFR_cyto

Use

AAV

Promoter

hSyn

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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