We narrowed to 24,073 results for: Sis

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pGWB507

Plasmid

#74849

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 6xHis (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV eIF4E W73A IRES GFP

Plasmid

#18762

Depositor

Scott Lowe

Article

Wendel et al Genes Dev. 2007 Dec 15. 21(24):3232-7.

Insert

eIF4E W73A (Eif4e Mouse)

Use

Retroviral

Tags

IRES GFP

Expression

Mammalian

Mutation

W73A

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
pGWB523

Plasmid

#74865

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with GST (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE476

Plasmid

#153267

Purpose

M2E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pFB-GTE-COI1

Plasmid

#29516

Depositor

Ning Zheng

Article

Sheard et al Nature. 2010 Nov 18. 468(7322):400-5.

Insert

COI1 (COI1 Mustard Weed)

Tags

GST and TEV cleavage site

Expression

Insect

Mutation

Note that the first AA in TEV site is R, not E

Available Since

May 26, 2011

Availability

Academic Institutions and Nonprofits only
pCS2+ macroH2A1.2-GFP-HA

Plasmid

#30515

Depositor

John Gurdon

Article

Pasque et al EMBO J. 2011 May 6;30(12):2373-87. doi:

Insert

macroH2A1.2-C (Macroh2a1 Mouse)

Use

Xenopus/zebrafish expression.

Tags

eGFP and triple HA

Expression

Mammalian

Mutation

NO STOP

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only

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pMRX-IBU-SNAP-Rab7

Plasmid

#228141

Purpose

Expresses SNAP-Rab7 in mammalian cells

Depositor

Noboru Mizushima

Article

Kurikawa et al PLoS Biol. 2024 Sep 17;22(9):e3002777. d

Insert

Rab7 (Rab7 Mouse)

Use

Retroviral

Tags

SNAP

Expression

Mammalian

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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YFP-Rac1(L61)

Plasmid

#11401

Depositor

Joel Swanson

Article

Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

Insert

ras-related C3 botulinum toxin substrate 1 (RAC1 Human)

Tags

YFP

Expression

Mammalian

Mutation

Obtained with Glutamine 61 mutated to Leucine.

Available Since

Feb. 21, 2007

Availability

Academic Institutions and Nonprofits only
FUW-M2-PAK2 wt

Plasmid

#31680

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

p21-activated protein kinase 2 (PAK2 Human)

Use

Lentiviral

Tags

M2

Expression

Mammalian

Available Since

April 12, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV-CMP-PE-V1

Plasmid

#202769

Purpose

Expresses CMP-PE-V1 in mammalian cells for prime editing

Depositor

Kyoungmi Kim

Article

Park et al Genome Biol. 2021 Jun 3;22(1):170. doi:

Insert

CMP-PE-V1

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO HSPA1L

Plasmid

#19457

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

HSPA1L (HSPA1L Human)

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-Rac1 G15A

Plasmid

#69355

Purpose

expression of nucleotide free Rac1 in bacterial cells

Depositor

Rafael Garcia-Mata

Article

Garcia-Mata et al Methods Enzymol. 2006;406:425-37.

Insert

Rac1 (RAC1 Human)

Tags

GST

Expression

Bacterial

Mutation

G15A

Promoter

tac

Available Since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only
p6899 PHAGE-P CMVT N-HA-hE6AP II C843A

Plasmid

#37604

Depositor

Peter Howley

Article

Martinez-Noel et al Mol Cell Biol. 2012 May 29.

Insert

E6AP II C843A (UBE3A Human)

Tags

HA

Expression

Mammalian

Mutation

C843A

Promoter

CMVt

Available Since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3-AktAR-Kras

Plasmid

#123357

Purpose

FRET-based biosensor for monitoring Akt activity in non-raft plasma membrane regions.

Depositor

Jin Zhang

Article

Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.

Insert

AktAR-Kras

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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ABI_cPPVp

Plasmid

#119212

Purpose

Expresses C-terminus of plum pox virus protease (PPVp) fused to the ABI-abscisic acid responsive protein domain

Depositor

Roman Jerala

Article

Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

Insert

split cPPVp in fusion with ABI

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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PYL1_nPPVp

Plasmid

#119211

Purpose

Expresses N-terminus of plum pox virus protease (PPVp) fused to PYL1-abscisic acid responsive protein domain

Depositor

Roman Jerala

Article

Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

Insert

split nPPVp in fusion with PYL1

Tags

AU1 tag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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optoPKAW196R/Y204A

Plasmid

#104546

Purpose

Expresses Cry2-mCherry-PKA W196R/Y204A in mammalian cells.

Depositor

David Lawrence

Article

O'Banion et al Cell Chem Biol. 2017 Oct 25. pii: S2451-

Insert

Cry2-mCherry-PKA W196R/Y204A

Tags

mCherry

Expression

Mammalian

Mutation

PKA W196R and Y204A

Promoter

CMV

Available Since

March 12, 2018

Availability

Academic Institutions and Nonprofits only

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pGWB423

Plasmid

#74817

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with GST (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

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FRB_nSbMVp

Plasmid

#118969

Purpose

Expresses N-terminus of soybean mosaic virus protease (SbMVp) fused to FKBP-rapamycin binding (FRB) domain

Depositor

Roman Jerala

Article

Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

Insert

split nSbMVp in fusion with FRB

Tags

Myc tag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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p7274 MSCV-P C-FlagHA 16 ∆DLYC

Plasmid

#163304

Purpose

Expresses HPV16 E7 ∆DLYC

Depositor

Elizabeth White

Article

Hatterschide et al Proc Natl Acad Sci U S A. 2019 Apr 2;116

Insert

HPV16 E7 ∆DLYC

Use

Retroviral

Tags

FlagHA

Mutation

∆DLYC

Promoter

MSCV LTR

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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