We narrowed to 120,297 results for: CIL
-
Plasmid#133802PurposeCMV and T7 promoter expression plasmid for human codon optimized AcrIIA5 with C-terminal NLS (SV40)DepositorInserthuman codon optimized AcrIIA5
TagsNLS(SV40)ExpressionMammalianMutationn/aPromoterCMV and T7Available SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Dyskerin
Plasmid#207577PurposeN-terminal mCherry-tag using SalI/EcoRIDepositorInsertgenerated by restriction cloning of gene into a modified pBABE-puro vector containing the N-terminal mCherrytag using SalI/EcoRI
TagsmCherryExpressionMammalianPromoterLTRAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-Halo-Dyskerin
Plasmid#207578PurposeCRISPR-Cas9 genome editing encoding a FLAG-HaloTag at the endogenous dyskerin locusDepositorInsertHaloTag DKC1
TagsHaloTagExpressionMammalianPromoterCMVAvailable SinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3
Plasmid#48743PurposeExpression of luciferase driven by truncated mPer2 promoter region (bases -1128 to +1060 with respect to transcription start site at +1)DepositorInserttruncated mPer2 promoter/enhancer region (-1128 to +1060)
UseLuciferaseMutationtruncated promoter region (see pGL6)Available SinceDec. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCALNL-NaChBac
Plasmid#125580PurposeCre-dependent expression of NaChBac (FLAG-tagged) in mammalian cells.DepositorInsertNaChBac
UseCre/LoxTagsFLAGExpressionMammalianAvailable SinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcpBpaRS
Plasmid#196484PurposeTo express benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into benzoylphenylalanine.DepositorInsertbenzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS), amber suppressor tRNA
ExpressionMammalianPromoterCMV (synthetase), Pol III tRNA promoter (tRNA)Available SinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBITE-EGFRvIII-F271-OKT3
Plasmid#190682PurposeEncodes an EGFRvIII-CD3 BITE protein (F271 clone)DepositorInsertAnti-EGFRvIII-scFv-F271-modularized-OKT3-HisTag
UseLentiviralTagsmNeonGreenExpressionMammalianPromoterEFSAvailable SinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
MSCVhygro-F-Ezh2-F667I
Plasmid#24927DepositorInsertEzh2 (EZH2 Mouse)
UseRetroviralTagsFLAGExpressionMammalianMutationPhenylanaline(F)667 mutated to Isoleucine(I)Available SinceJuly 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-FLAG-Bbs4
Plasmid#128868PurposeFor mammalian expression of mouse Bbs4DepositorAvailable SinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ER-CaNAR2
Plasmid#64732PurposeEndoplasmic reticulum (ER)-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of ER membrane.DepositorInsertCYP450-CaNAR2
TagsCerulean3, N-terminal 27 amino acids from CYP450,…ExpressionMammalianPromoterCMVAvailable SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
PADH1-TIR1-9myc-URA3
Plasmid#207016PurposeExpresses TIR1-9Myc. TIR1 mediates auxin-induced ubiquitination of auxin-inducible degron (AID).DepositorInsertTIR1
Tags9xMycExpressionYeastPromoterpADH1Available SinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF5/FRT-DEST EVC2-Flag
Plasmid#41005DepositorAvailable SinceNov. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PM-CaNAR2
Plasmid#64730PurposePlasma membrane-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.DepositorInsertLyn-CaNAR2
TagsCerulean3, N-terminal targeting sequence from Lyn…ExpressionMammalianPromoterCMVAvailable SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
PDZD7-TwinStrepII
Plasmid#83198PurposeExpression of tagged protein in mammalian cellsDepositorAvailable SinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBluescript-IC3-FLAG-Tev-ZZ
Plasmid#170311PurposePlasmid for endogenous C-terminal tagging of the Tetrahymena thermophila Outer Dynein Arm intermediate chain 3 with FLAG and ZZ tags.DepositorInsertOuter Dynein Arm Intermediate Chain 3
TagsFLAG, ZZAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3 Pro E2
Plasmid#48749PurposeExpression of luciferase driven by mPer2 promoter fragment containing E-box2 (bases -112 to +98 with respect to transcription start site at +1) and SV40 promoter (backbone)DepositorInsertmPer2 promoter/enhancer region containing E-box2 (-112 to +98)
UseLuciferaseAvailable SinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
BS-TRP1-TetOff(7 repeats)-mRuby3-Pho8
Plasmid#207031PurposeTet-Off controlled mRuby3-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.DepositorInsertPho8
TagsmRuby3ExpressionYeastAvailable SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
Halo-TCAB1 HRD
Plasmid#207535PurposeHomologous recombination donor to insert HaloTag at the N-terminus of the endogenous TCAB1 locus.DepositorInsertHaloTag with internal PuroR cassette flanked by human TCAB1 locus sequences
TagsHaloTagExpressionMammalianPromoterEndogenousAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Mito-CaNAR2
Plasmid#64731PurposeMitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of mitochondrial outer membrane.DepositorInsertCaNAR2
TagsCerulean3, N-terminal 30 amino acids from DAKAP1,…ExpressionMammalianPromoterCMVAvailable SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTK331
Plasmid#59572PurposeTC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(14) Kozak mKate-G8-L7Ae-PEST 4xFF4 attB2 SGP2(98/30) XbaI attB1 EBFP2 ochre attB2 (insert) AscI (truncated E1) 3'UTR Poly A I-SceIDepositorInsertSGP(16) 2xK-turn Kozak EGFP attB2 AscI
Available SinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only