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pET-28b-RfxCas13d-His

Plasmid

#141322

Purpose

Plasmid for bacterial expression and purification of RfxCas13d protein

Depositor

Ariel Bazzini, Miguel Angel Moreno-Mateos

Article

Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

Insert

Rfx-Cas13d

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pT3TS-RfxCas13d-NLS-HA

Plasmid

#141321

Purpose

Plasmid to carry out IVT of RfxCas13d-NLS (human codon-optimized)

Depositor

Ariel Bazzini, Miguel Angel Moreno-Mateos

Article

Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

Insert

Rfx-Cas13d (NLS-RfxCas13d-NLS)

Use

CRISPR

Tags

HA and NLS

Expression

Mutation

Promoter

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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PB-PG13-mCherry-EF1a-PuroR-EGFP

Plasmid

#195292

Purpose

piggyBac transposon vector with mCherry reporter containing 13 copies of the p53-binding consensus sequence (constitutive puromycin resistance and EGFP)

Depositor

Hideyuki Oguro

Article

Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

Inserts

mCherry
PuroR-P2A-EGFP

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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Slax mMyoD (CT#999)

Plasmid

#13897

Depositor

Cliff Tabin

Article

Brent et al Cell. 2003 Apr 18. 113(2):235-48.

Insert

MyoD (Myod1 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 16, 2007

Availability

Academic Institutions and Nonprofits only
pDSG594

Plasmid

#214221

Purpose

competition strain 10 integrase plasmid

Depositor

Uri Alon

Article

Glass et al Cell. 2024 Jan 31:S0092-8674(24)00061-8.

Insert

PhlF, A118

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pDSG578

Plasmid

#214215

Purpose

integrase plasmid

Depositor

Uri Alon

Article

Glass et al Cell. 2024 Jan 31:S0092-8674(24)00061-8.

Insert

PhlF, A118

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pDSG602

Plasmid

#214229

Purpose

BAC target plasmid

Depositor

Uri Alon

Article

Glass et al Cell. 2024 Jan 31:S0092-8674(24)00061-8.

Insert

AraC, folA, trpC

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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pDSG467

Plasmid

#214209

Purpose

Arabinose-induced trpC

Depositor

Uri Alon

Article

Glass et al Cell. 2024 Jan 31:S0092-8674(24)00061-8.

Insert

AraC, trpC

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pDSG597

Plasmid

#214224

Purpose

competition strain 9 integrase plasmid

Depositor

Uri Alon

Article

Glass et al Cell. 2024 Jan 31:S0092-8674(24)00061-8.

Insert

PhlF, A118

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pDSG596

Plasmid

#214223

Purpose

competition strain 6 integrase plasmid

Depositor

Uri Alon

Article

Glass et al Cell. 2024 Jan 31:S0092-8674(24)00061-8.

Insert

PhlF, A118

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pDSG590

Plasmid

#214218

Purpose

competition strain 8 integrase plasmid

Depositor

Uri Alon

Article

Glass et al Cell. 2024 Jan 31:S0092-8674(24)00061-8.

Insert

PhlF, A118

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pDSG595

Plasmid

#214222

Purpose

competition strain 2 integrase plasmid

Depositor

Uri Alon

Article

Glass et al Cell. 2024 Jan 31:S0092-8674(24)00061-8.

Insert

PhlF, A118

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pDSG598

Plasmid

#214225

Purpose

competition strain 1 integrase plasmid

Depositor

Uri Alon

Article

Glass et al Cell. 2024 Jan 31:S0092-8674(24)00061-8.

Insert

PhlF, A118

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pDSG599

Plasmid

#214226

Purpose

competition strain 5 integrase plasmid

Depositor

Uri Alon

Article

Glass et al Cell. 2024 Jan 31:S0092-8674(24)00061-8.

Insert

PhlF, A118

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pDSG600

Plasmid

#214227

Purpose

competition strain 3 integrase plasmid

Depositor

Uri Alon

Article

Glass et al Cell. 2024 Jan 31:S0092-8674(24)00061-8.

Insert

PhlF, A118

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pDSG601

Plasmid

#214228

Purpose

competition strain 4 integrase plasmid

Depositor

Uri Alon

Article

Glass et al Cell. 2024 Jan 31:S0092-8674(24)00061-8.

Insert

PhlF, A118

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pDSG593

Plasmid

#214220

Purpose

competition strain 11 integrase plasmid

Depositor

Uri Alon

Article

Glass et al Cell. 2024 Jan 31:S0092-8674(24)00061-8.

Insert

PhlF, A118

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pDSG591

Plasmid

#214219

Purpose

competition strain 7 integrase plasmid

Depositor

Uri Alon

Article

Glass et al Cell. 2024 Jan 31:S0092-8674(24)00061-8.

Insert

PhlF, A118

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pDSG488

Plasmid

#214210

Purpose

DAPG-inducible folA

Depositor

Uri Alon

Article

Glass et al Cell. 2024 Jan 31:S0092-8674(24)00061-8.

Insert

PhlF, TmpR

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBeloBac11loxP2272

Plasmid

#60342

Purpose

This is a BAC vector that contains both wt and mutant loxP sites.

Depositor

Jon Coren

Article

Coren et al BMC Res Notes. 2017 Jul 28;10(1):344. do

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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