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We narrowed to 6,450 results for: siae

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Showing: 1681 - 1710 of 6450 results

  4. pET151-Vps4(101-437)-Hcp1 E322A

    Plasmid
    #87748
    Purpose
    Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1
    Depositor
    Chris Hill
    Article
    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1
    Insert
    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    Tags
    6xHis-V5-TEV and Hcp1
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-100, mutated Glu-322 to Ala
    Promoter
    T7
    Available Since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    87748-plasmid-map-sequence-id-175376

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  5. pET151-Vps4(101-437)-Hcp1 R325A

    Plasmid
    #87749
    Purpose
    Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1
    Depositor
    Chris Hill
    Article
    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1
    Insert
    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    Tags
    6xHis-V5-TEV and Hcp1
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-100, mutated Arg-325 to Ala
    Promoter
    T7
    Available Since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    87749-plasmid-map-sequence-id-175377

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  6. pET151-Vps4(101-437)-Hcp1 K321D

    Plasmid
    #89288
    Purpose
    Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1
    Depositor
    Chris Hill
    Article
    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1
    Insert
    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    Tags
    6xHis-V5-TEV and Hcp1
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-100, mutated Lys-321 to Asp
    Promoter
    T7
    Available Since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    89288-plasmid-map-sequence-id-175369

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  7. pET151-Vps4(101-437)-Hcp1 R325D

    Plasmid
    #89289
    Purpose
    Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1
    Depositor
    Chris Hill
    Article
    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1
    Insert
    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    Tags
    6xHis-V5-TEV and Hcp1
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-100, mutated Arg-325 to Asp
    Promoter
    T7
    Available Since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    89289-plasmid-map-sequence-id-175352

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  8. pET151-Vps4(101-437)-Hcp1 K321A

    Plasmid
    #87747
    Purpose
    Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1
    Depositor
    Chris Hill
    Article
    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1
    Insert
    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    Tags
    6xHis-V5-TEV and Hcp1
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-100, mutated Lys-321 to Ala
    Promoter
    T7
    Available Since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    87747-plasmid-map-sequence-id-175355

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  13. pC-BAR-YR

    Plasmid
    #61766
    Purpose
    Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing bar gene (for resistance against glufosinate ammonium) on transfer DNA (TDNA).
    Depositor
    Ken Haynes
    Article
    Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do
    Inserts
    Bar gene
    2 micron origin or replication and URA3 gene for S. cerevisiae
    Use
    Ternary vector for agrobacterium mediated transfo…
    Promoter
    trpC promoter
    Available Since
    May 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    61766-plasmid-map-sequence-id-442755

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  14. pSUMO-β′-COPI-WD40

    Plasmid
    #180753
    Purpose
    Bacterial expression plasmid for Saccharomyces cerevisiae β′-COPI-WD40 (residues 1-301).
    Depositor
    Syed Saif Hasan
    Article
    Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1
    Insert
    β′-COPI-WD40 (SEC27 Budding Yeast)
    Tags
    N-terminal strep tag, His-tag, and SUMO tag
    Expression
    Bacterial
    Mutation
    Encodes residues 1-301; deleted 302-889; mutated …
    Promoter
    T7
    Available Since
    March 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    180753-plasmid-map-sequence-id-356139

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  15. kanMX6-ins6

    Plasmid
    #195043
    Purpose
    pFA6a derived selection cassette 5' flanked with tDEG1 transcription terminator, allows genome modification without disruption of insertion neighboring genes by transcription interference
    Depositor
    Gloria Brar
    Article
    Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75
    Insert
    KanR
    Use
    Yeast genomic targeting
    Tags
    S. cerevisiae DEG1 terminator, A. gossypii TEF pr…
    Available Since
    Feb. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    195043-plasmid-map-sequence-id-384247

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  17. natMX-ins7

    Plasmid
    #195044
    Purpose
    pFA6a derived selection cassette 5' flanked with tCYC1 transcription terminator, allows genome modification without disruption of insertion neighboring genes by transcription interference
    Depositor
    Gloria Brar
    Article
    Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75
    Insert
    NatR
    Use
    Yeast genomic targeting
    Tags
    tCYC1 S. cerevisiae, pPGK1 C. glabrata, NatR, tPG…
    Available Since
    Feb. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    195044-plasmid-map-sequence-id-384248

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  21. pZK12

    Plasmid
    #248912
    Purpose
    expresses yeast Isc10 fused to maltose binding protein and Cak1 in bacteria
    Depositor
    Edward Winter
    Article
    Rimal et al Mol Cell Biol. 2020 Jul 29;40(16). pii:
    Inserts
    Isc10-MBP (ISC10 Budding Yeast, Sequence is optimized for expression of S. cerevisiae Isc10 sequence in bacteria)
    Cak1 (CAK1 Budding Yeast)
    Tags
    maltose binding protein
    Expression
    Bacterial
    Mutation
    Nucleotide sequence is optimized for bacterial ex…
    Promoter
    T7
    Available Since
    Jan. 16, 2026
    Availability
    Academic Institutions and Nonprofits only
    248912-plasmid-map-sequence-id-497720

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  26. pdnCas3-APOBEC1

    Plasmid
    #229537
    Purpose
    pMV_hyg encoding dnCas3(H74A+D75A)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning
    Depositor
    Kevin Verstrepen
    Article
    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:
    Inserts
    rAPOBEC1
    dnCas3
    Uracil glycosylase inhibitor
    Tags
    XTEN
    Expression
    Bacterial and Yeast
    Mutation
    codon optimized for S.cerevisiae and codon optimi…
    Available Since
    Jan. 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    229537-plasmid-map-sequence-id-459005

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Showing: 1681 - 1710 of 6450 results