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pCAG-1700029I15Rik-3×FLAG

Plasmid

#199689

Purpose

Expression vector of mouse 1700029I15Rik. 3XFLAG tag at C-terminus.

Depositor

Masahito Ikawa

Article

Lu et al Proc Natl Acad Sci U S A. 2023 Feb 21;12

Insert

1700029I15Rik (1700029I15Rik Mouse)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG.Myc-SARS2_3CL(C145A)

Plasmid

#200121

Purpose

Expresses N-terminal Myc-tagged human codon optimyzed SARS-CoV-2 3CL C145A in mammalian cells

Depositor

Eiji Morita

Article

Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

Insert

SARS-CoV-2 3CL protease (ORF1ab SARS-CoV-2)

Use

Tags

Myc

Expression

Mammalian

Mutation

C145A

Promoter

CAG

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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AP76_pU.CAG.Cas9-D10A-K848A.rBGpA

Plasmid

#199253

Purpose

Expression construct encoding the high specificity SpCas9-KA-D10A nickase

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

Insert

High specificity S. pyogenes Cas9-D10A-KA nickase

Use

CRISPR

Tags

3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Mutation

D10A K848A

Promoter

CAG promoter

Available since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG1.1-Tsks/1D4

Plasmid

#199623

Purpose

Expression vector of mouse TSKS (variant X6). 1D4 tag at C-terminus.

Depositor

Masahito Ikawa

Article

Shimada et al Proc Natl Acad Sci U S A. 2023 Mar 14;12

Insert

Tsks (variant X6) (Tsks Mouse)

Use

Tags

1D4

Expression

Mutation

Promoter

CAG

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mIFP

Plasmid

#197214

Purpose

Expresses the protein of mIFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

mIFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

April 4, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-iRFP713

Plasmid

#197209

Purpose

Expresses the protein of iRFP713 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

iRFP713

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-BDFP1.8

Plasmid

#197206

Purpose

Expresses the protein of BDFP1.8 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

BDFP1.8

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-iRFP682

Plasmid

#197204

Purpose

Expresses the protein of iRFP682 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

iRFP682

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mRhubarb719

Plasmid

#197211

Purpose

Expresses the protein of mRhubarb719 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

mRhubarb719

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-miRFP2

Plasmid

#197216

Purpose

Expresses the protein of miRFP2 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP2

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-IFP2

Plasmid

#197215

Purpose

Expresses the protein of IFP2 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

IFP2

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-GFP-Rab27a

Plasmid

#194985

Purpose

overexpress Rab27a

Depositor

Angelique Bordey

Insert

GFP-Rab27a

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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AV0.1-CAG-WtSV

Plasmid

#192891

Purpose

Cloning vector

Depositor

Kazuhiro Oka

Type

Empty backbone

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-3xHAtag-SP-CLSTN2

Plasmid

#191694

Purpose

For expression coding sequencing of CLSTN2

Depositor

Yuejun Chen

Article

Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

Insert

signal peptide, 3xHAtag and full length coding sequence of CLSTN2

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-SP-3xHAtag-PTPRO

Plasmid

#191695

Purpose

For expression coding sequencing of PTPRO

Depositor

Yuejun Chen

Article

Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

Insert

signal peptide, full length coding sequence of PTPRO and 3xHAtag

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pD2529-CAG-b3 N305T

Plasmid

#189798

Purpose

Expression of full length human integrin beta 3 with N305T

Depositor

Timothy Springer

Article

Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

Insert

integrin b3_N305T (ITGB3 Human)

Use

Tags

-P2A-mCherry

Expression

Mammalian

Mutation

N305T

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-MBP-ATG9

Plasmid

#188076

Purpose

Recombinant overexpression of human ATG9 in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Use

Tags

MBP tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-ATG13 (1-197)

Plasmid

#188074

Purpose

To make ATG101/ATG13 HORMA complex for expression in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG13 (ATG13 Human)

Use

Tags

Expression

Mammalian

Mutation

codon-optimized ATG13 (1-197)

Promoter

CMV

Available since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-mTurbo-mito

Plasmid

#187177

Purpose

Express mitochondira-localized miniTurbo under the control of CAG promoter

Depositor

Isei Tanida

Article

Sanada et al Sci Rep. 2022 Jul 1;12(1):11130. doi: 10

Insert

mtActA fused with miniTurbo (BirA mutant)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-H2B-mTurbo

Plasmid

#187178

Purpose

Express histone H2B tagged with miniTurbo under the control of CAG promoter

Depositor

Isei Tanida

Article

Sanada et al Sci Rep. 2022 Jul 1;12(1):11130. doi: 10

Insert

histone H2B fused with miniTurbo (BirA mutant)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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