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SARS-CoV-2_N_deltaNIDR

Plasmid

#157868

Purpose

express MBP tagged SARS-CoV-2 Nucleocapsid protein without the N-terminal IDR

Depositor

Nicolas Fawzi

Article

Perdikari et al EMBO J. 2020 Nov 17:e106478. doi: 10.152

Insert

N (N SARS-CoV-2)

Use

Tags

MBP

Expression

Bacterial

Mutation

deltaNIDR

Promoter

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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TET-pLKO.1 PURO shGFP

Plasmid

#83085

Purpose

Lentiviral shRNA vector for inducible knockdown of GFP (control hairpin)

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
TET-pLKO.1 PURO shLacZ

Plasmid

#98632

Purpose

Lentiviral shRNA vector for inducible knockdown of LacZ (control hairpin)

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shLacZ

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pAL1

Plasmid

#197285

Purpose

Shuttle plasmid that replicates in E. coli, S. cerevisiae, and A. laidlawii. Contains the OriC from A. laidlawii 8195

Depositor

Bogumil Karas

Article

Nucifora et al ACS Synth Biol. 2024 Jan 19;13(1):45-53.

Insert

Origin of replication of A. laidlawii 8195: whole dnaA gene, plus upstream and downstream intergenic regions

Use

Synthetic Biology; Bacterial and yeast cloning

Tags

Expression

Mutation

Promoter

Available since

April 6, 2023

Availability

Academic Institutions and Nonprofits only

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TRAPa-3xFLAG-pCDNA

Plasmid

#201963

Purpose

Mammalian expression of Translocon-associated protein subunit alpha

Depositor

Ville Paavilainen

Article

Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

Insert

SSR1 (SSR1 Human)

Use

Tags

3xFLAG-tag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 4, 2024

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2_N_deltaCIDR

Plasmid

#157872

Purpose

express MBP tagged SARS-CoV-2 Nucleocapsid protein without the C-terminal IDR

Depositor

Nicolas Fawzi

Article

Perdikari et al EMBO J. 2020 Nov 17:e106478. doi: 10.152

Insert

N (N SARS-CoV-2)

Use

Tags

MBP

Expression

Bacterial

Mutation

deltaCIDR

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pPT-PCR C2

Plasmid

#141247

Purpose

PCR cloned Phaeodactylum tricornutum Mitochondrial Genome - Clone 2

Depositor

Bogumil Karas

Article

Cochrane et al Algal Research, 2020. 50, 102014

Insert

Mitochondrial Genome of Phaeodactylum tricornutum

Use

Synthetic Biology; Diatom expression

Tags

Expression

Bacterial and Yeast

Mutation

Repeat region removed, additional minor mutations…

Promoter

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pXJ40 GADD34 (Delta Pest1)

Plasmid

#78096

Purpose

mammalian expression of GADD34 Delta Pest1

Depositor

Shirish Shenolikar

Article

Choy et al Cell Rep. 2015 Jun 30;11(12):1885-91. do

Insert

GADD34 (PPP1R15A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

deletion of PEST1

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pSLIK TT 3xFLAG Luciferase neo

Plasmid

#98392

Purpose

Lentiviral vector for inducible expression of Luciferase with 3xFLAG N-terminal tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

Luciferase

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCRRNA-G0-R0

Plasmid

#101803

Purpose

Repress mCherry and GFP independently, with different strengths

Depositor

Sven van Teeffelen, David Bikard

Article

Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

Insert

crRNAs targeting GFP and mCherry

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Spy 1049

Available since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pXJ40 GADD34 (514-674aa)

Plasmid

#75480

Purpose

mammalian expression of GADD34 514-674 aa

Depositor

Shirish Shenolikar

Article

Choy et al Cell Rep. 2015 Jun 30;11(12):1885-91. do

Insert

GADD34 (PPP1R15A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

expresses aa 514-674

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only
Split-RESCUE-C

Plasmid

#170154

Purpose

AAV vector carrying the RESCUE-DDC-λN (RESCUE is the evolved ADAR2 variant for C-U editing) with a nuclear export signal

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

RESCUE-DDC-λN-NES

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pYFP-lacY-.008

Plasmid

#140140

Purpose

Evaluating the impact of feedback and low sensor concentration on information transmission of a genetic sensor.

Depositor

Drew Tack

Article

Sarkar et al Commun Biol. 2020 Apr 30;3(1):203. doi:

Insert

lacY (lacY )

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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pXJ40 GADD34 (1-513aa)

Plasmid

#75479

Purpose

mammalian expression of GADD34 1-513 aa

Depositor

Shirish Shenolikar

Article

Choy et al Cell Rep. 2015 Jun 30;11(12):1885-91. do

Insert

GADD34 (PPP1R15A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

expresses aa 1-513

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only
pXJ40 GADD34 (214-513aa)

Plasmid

#78095

Purpose

mammalian expression of GADD34 214-513 aa

Depositor

Shirish Shenolikar

Article

Choy et al Cell Rep. 2015 Jun 30;11(12):1885-91. do

Insert

GADD34 (PPP1R15A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

expresses aa 214-513

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only
pSLIK TT 3xFLAG LacZ neo

Plasmid

#83105

Purpose

Lentiviral vector for inducible expression of LacZ with 3xFLAG N-terminal tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

LacZ

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pSLIK TT 3xFLAG LacZ hygro

Plasmid

#98395

Purpose

Lentiviral vector for inducible expression of LacZ with 3xFLAG N-terminal tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

LacZ

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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pEN_TT miRc2 3xFLAG

Plasmid

#83274

Purpose

Empty entry vector for cloning inducible mammalian expression and an in-frame N-terminal 3xFLAG

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Type

Empty backbone

Use

Entry vector for gateway cloning

Tags

3xFLAG

Expression

Mutation

Promoter

TRE tight

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pPROEX Hsp104 (773-908)

Plasmid

#1316

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 (773-908) (HSP104 Budding Yeast)

Use

Tags

6X His + TEV cleavage

Expression

Bacterial

Mutation

deletion of aa1-773

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pPROEX Hsp104 (361-548)

Plasmid

#1317

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 (361-548) (HSP104 Budding Yeast)

Use

Tags

6X His + TEV cleavage

Expression

Bacterial

Mutation

aa361-548 of Hsp104

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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