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Showing: 1121 - 1140 of 1231 results

  6. pcDNA3-Lyn-dPlcR

    Plasmid
    #181832
    Purpose
    Genetically encoded single-color sensor for monitoring plasma membrane PI(4,5)P2 dynamics in living cells. Based on dimerization-dependent green fluorescent protein (ddGFP).
    Depositor
    Jin Zhang
    Article
    Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.
    Insert
    Lyn-PlcR
    Use
    Tags
    N-terminal targeting sequence from Lyn kinase, dd…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    181832-plasmid-map-sequence-id-364041

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  11. Lck-mScarlet-I

    Plasmid
    #98821
    Purpose
    In vivo visualization of the plasma membrane (can be used for colocalization studies)
    Depositor
    Dorus Gadella
    Article
    Chertkova et al bioRxiv 160374
    Insert
    Lck
    Use
    Tags
    mScarlet-I
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    98821-plasmid-map-sequence-id-358010

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  13. Lck-mTurquoise2

    Plasmid
    #98822
    Purpose
    In vivo visualization of the plasma membrane (can be used for colocalization studies)
    Depositor
    Dorus Gadella
    Article
    Chertkova et al bioRxiv 160374
    Insert
    Lck
    Use
    Tags
    mTurquoise2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    98822-plasmid-map-sequence-id-190902

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  14. pDS275

    Plasmid
    #34972
    Purpose
    Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(T406A, T407A, I532A mutations).
    Depositor
    Michael Glotzer
    Article
    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm
    Insert
    Mid2(SS/TM)-GFP-LOVpep
    Use
    Tags
    Expression
    Yeast
    Mutation
    T406A, T407A, I532A
    Promoter
    Available since
    March 23, 2012
    Availability
    Academic Institutions and Nonprofits only
    34972-plasmid-map-sequence-id-42929

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  15. CIBN-CAAX

    Plasmid
    #79574
    Purpose
    expression of N-terminal portion of CIB1 with a C-terminal CAAX box from KRras for plasma-membrane targeting
    Depositor
    Olof Idevall-Hagren, Pietro De Camilli
    Article
    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10
    Insert
    CIBN (CIB1 Mustard Weed)
    Use
    Tags
    C-terminal CAAX-box
    Expression
    Mammalian
    Mutation
    K93A, R94A, K106A, K107A (please see depositor co…
    Promoter
    CMV
    Available since
    July 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    79574-plasmid-map-sequence-id-314173

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  16. Lyn-tdnano-HA

    Plasmid
    #136512
    Purpose
    Mammalian expression of a tandem dimer of WT SspB (nano), targeted to membrane by Lyn myristoylation sequence, with triple-HA epitope tag
    Depositor
    Bianxiao Cui
    Article
    Hope et al J Mol Biol. 2020 Jun 12;432(13):3739-374
    Insert
    Lyn nano nano 3HA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    136512-plasmid-map-sequence-id-271004

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  19. pDS240

    Plasmid
    #34966
    Purpose
    Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(K533∆, high-affinity epitope).
    Depositor
    Michael Glotzer
    Article
    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm
    Insert
    Mid2(SS/TM)-GFP-LOVpep
    Use
    Tags
    Expression
    Yeast
    Mutation
    K533∆, high-affinity epitope
    Promoter
    GAL1
    Available since
    March 21, 2012
    Availability
    Academic Institutions and Nonprofits only
    34966-plasmid-map-sequence-id-42500

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Showing: 1121 - 1140 of 1231 results