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pPR-1_2A_LD5N

Plasmid

#250454

Purpose

GRASP module to assemble sPPR proteins for RNA editing: module 2A_LD5N

Depositor

Ian Small

Article

Dennis et al Nucleic Acids Res. 2025 Oct 28;53(20):gk

Insert

2A_LD5N

Use

Synthetic Biology

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pPR-1_1E_LN5T

Plasmid

#250453

Purpose

GRASP module to assemble sPPR proteins for RNA editing: module 1E_LN5T

Depositor

Ian Small

Article

Dennis et al Nucleic Acids Res. 2025 Oct 28;53(20):gk

Insert

1E_LN5T

Use

Synthetic Biology

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pPR-1_1E_LN5N

Plasmid

#250452

Purpose

GRASP module to assemble sPPR proteins for RNA editing: module 1E_LN5N

Depositor

Ian Small

Article

Dennis et al Nucleic Acids Res. 2025 Oct 28;53(20):gk

Insert

1E_LN5N

Use

Synthetic Biology

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pPR-1_1E_LD5T

Plasmid

#250451

Purpose

GRASP module to assemble sPPR proteins for RNA editing: module 1E_LD5T

Depositor

Ian Small

Article

Dennis et al Nucleic Acids Res. 2025 Oct 28;53(20):gk

Insert

1E_LD5T

Use

Synthetic Biology

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pPR-1_1E_LD5N

Plasmid

#250450

Purpose

GRASP module to assemble sPPR proteins for RNA editing: module 1E_LD5N

Depositor

Ian Small

Article

Dennis et al Nucleic Acids Res. 2025 Oct 28;53(20):gk

Insert

1E_LD5N

Use

Synthetic Biology

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pPR-1_1A_5T_AGGT

Plasmid

#250449

Purpose

GRASP module to assemble sPPR proteins for RNA editing: module 1A_5T_AGGT

Depositor

Ian Small

Article

Dennis et al Nucleic Acids Res. 2025 Oct 28;53(20):gk

Insert

1A_5T_AGGT

Use

Synthetic Biology

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pPR-1_1A_5N_AGGT

Plasmid

#250447

Purpose

GRASP module to assemble sPPR proteins for RNA editing: module 1A_5N_AGGT

Depositor

Ian Small

Article

Dennis et al Nucleic Acids Res. 2025 Oct 28;53(20):gk

Insert

1A_5N_AGGT

Use

Synthetic Biology

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pPR-1_1A_5N_AATG

Plasmid

#250446

Purpose

GRASP module to assemble sPPR proteins for RNA editing: module 1A_5N_AATG

Depositor

Ian Small

Article

Dennis et al Nucleic Acids Res. 2025 Oct 28;53(20):gk

Insert

1A_5N_AATG

Use

Synthetic Biology

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pQE30::CLOMELEON:Strep

Plasmid

#247989

Purpose

Expression of the indicator protein CLOMELEON in E. coli.

Depositor

Christoph Plieth

Article

Lorenzen et al Plant J. 2004 May;38(3):539-44. doi: 10.

Insert

CLOMELEON (CFP-YFP fusion with ENLYFQG-linker)

Tags

6xHis and Strep

Expression

Bacterial

Mutation

CFP-variant: K26R, F64L, S65T, Y66W, N146I, M153T…

Promoter

T5-phage promoter

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pEvolvR-mCherry-nCas9-PolI5M

Plasmid

#249066

Purpose

Expresses nucleus-localized nCas9 (D10A) fused to PolI5M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

nCas9-PolI5M

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pEvolvR-mCherry-enCas9(D10A)-PolI5M

Plasmid

#249060

Purpose

Expresses nucleus-localized enCas9 (D10A) fused to PolI5M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

enCas9-PolI5M

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 23, 2025

Availability

Academic Institutions and Nonprofits only

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p-mCherry-dCas9-PolI5M

Plasmid

#249065

Purpose

Expresses nucleus-localized dCas9 fused to PolI5M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

dCas9-PolI5M

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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p-mCherry-enCas9(D10A)

Plasmid

#249063

Purpose

Expresses nucleus-localized enCas9 (D10A) fused to mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

enCas9

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pBAD_LcgcC-GFP-H6

Plasmid

#245528

Purpose

His-tagged superfolder GFP C-terminally fused to C-terminal LCGC14 split intein fragment

Depositor

Henning Mootz

Article

Humberg et al Angew Chem Int Ed Engl. 2025 Oct 23:e202

Insert

C-terminal Fragment of the LCGC14 PolB2 Split Intein

Tags

Hexahistidine Tag and Superfolder Green Fluoresce…

Expression

Bacterial

Available Since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pDonr201 RNF146

Plasmid

#240330

Purpose

Entry vector for Gateway with RNF146

Depositor

Junjie Chen

Article

Nie et al Mol Cell Proteomics. 2020 Dec;19(12):201

Insert

RNF146 (RNF146 Human)

Expression

Bacterial

Available Since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-HyPerFLEX-NLSx3-WPRE

Plasmid

#217563

Purpose

Mammalian expression of HyPerFLEX targeted to the nucleus

Depositor

Vsevolod Belousov

Article

Potekhina et al Nat Chem Biol. 2025 Oct 16. doi: 10.1038

Insert

HyPerFLEX-NLS

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-CaMKII-HyPerFLEX-UnaG-WPRE

Plasmid

#217564

Purpose

Expression of HyPerFLEX fused with UnaG in mammalian neurons

Depositor

Vsevolod Belousov

Article

Potekhina et al Nat Chem Biol. 2025 Oct 16. doi: 10.1038

Insert

HyPerFLEX-UnaG

Use

AAV

Expression

Mammalian

Promoter

CaMKII

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-HyPerFLEX-UnaG-WPRE

Plasmid

#217565

Purpose

Mammalian expression of HyPerFLEX fused with UnaG

Depositor

Vsevolod Belousov

Article

Potekhina et al Nat Chem Biol. 2025 Oct 16. doi: 10.1038

Insert

HyPerFLEX-UnaG

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-HyPerFLEX-NES-WPRE

Plasmid

#217562

Purpose

Mammalian expression of HyPerFLEX targeted to the cytoplasm

Depositor

Vsevolod Belousov

Article

Potekhina et al Nat Chem Biol. 2025 Oct 16. doi: 10.1038

Insert

HyPerFLEX-NES

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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XAC1 S3A,Y82F

Plasmid

#245949

Purpose

Expressing constitutively active XAC1 with lower turnover rate

Depositor

James Bamburg

Article

Abe et al J Cell Biol. 1996 Mar;132(5):871-85. doi

Insert

cofilin 1 (cfl1) (cfl1.L Frog)

Use

Frog expression

Mutation

S3A, Y82F

Promoter

CMV

Available Since

Oct. 22, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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