161,152 results

1. pAAV-nEF Con/Fon DREADD Gq-mCherry

   Plasmid

   #183532

   Purpose

   INTRSECT Chemogenetics - expresses DREADD Gq-mCherry in cells expressing Cre AND Flp

   Depositor

   Karl Deisseroth

   Insert

   DREADD Gq-mCherry
   

   Use

   AAV

   Tags

   Expression

   Mutation

   None

   Promoter

   nEF

   Available since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF1520

   Plasmid

   #141833

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NR2E1 (NR2E1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223_LacZ

   Plasmid

   #25893

   Depositor

   David Root

   Article

   Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

   Insert

   LacZ
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCoPURO

   Plasmid

   #17533

   Depositor

   Francis Castellino

   Article

   Iwaki et al Biotechniques. 2003 Sep . 35(3):482-4, 4

   Insert

   Puromycine Acetyl Transferase
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 21, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCB-1

   Plasmid

   #210387

   Purpose

   All-in-one cumate-based inducible CRISPRi plasmid for use in Streptomyces

   Depositor

   Gilles van Wezel

   Article

   Bai et al ACS Synth Biol. 2023 Oct 6. doi: 10.1021

   Inserts

   dCas9
   CymR
   sgRNA cassette
   

   Use

   CRISPR; Integrative vector for use in streptomyces

   Tags

   Expression

   Mutation

   BsaI-flanked RFP cassette inserted in place of sp…

   Promoter

   SP11 and SP30

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-TLR10-YFP

   Plasmid

   #13643

   Depositor

   Doug Golenbock

   Insert

   Toll-Like Receptor 10 (TLR10 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-CAG-jGCaMP8m-WPRE

   Plasmid

   #179255

   Purpose

   AAV-mediated expression of GCaMP8m under the CAG promoter

   Depositor

   Loren Looger

   Has Service

   AAV Retrograde, AAV1, and AAV9

   Insert

   jGCaMP8m
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA3-HA-Akt1

   Plasmid

   #73408

   Purpose

   Expresses human AKT1 with a N-terminus HA tag

   Depositor

   Jie Chen

   Article

   Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

   Insert

   AKT1 (AKT1 Human)
   

   Use

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. dCas9-miniTurbo

   Plasmid

   #219822

   Purpose

   EF1α-driven expression of dCas9 fused to miniTurbo with GFP (Adapted from plasmid #153209)

   Depositor

   Philipp Maass

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   miniTurbo

   Expression

   Mammalian

   Mutation

   D10A/H841A

   Promoter

   EF1a

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSL1765 (pSPIN-R, R*MmeI)

    Plasmid

    #160734

    Purpose

    Single-plasmid V. cholerae CAST. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA with BsaI sites. Reverse (R) order minimizes self-targeting; MmeI site for Tn-seq; pBBR1 backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST proteins, crRNA, and donor DNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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