162,981 results

1. pOPINB-OTULIN (full-length, aa 1-352)

   Plasmid

   #61464

   Purpose

   Expresses human OTULIN (full-length) in E. coli.

   Depositor

   David Komander

   Article

   Keusekotten et al Cell. 2013 Jun 6;153(6):1312-26. doi: 10

   Insert

   OTULIN (OTULIN Human)
   

   Use

   Tags

   His6-3C

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDGE503

   Plasmid

   #153276

   Purpose

   Intermediate sgRNA array cloning vector position 1

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDGE504

   Plasmid

   #153277

   Purpose

   Intermediate sgRNA array cloning vector position 2

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDGE508

   Plasmid

   #153280

   Purpose

   End-linker for cloning 2 sgRNA arrays into any recipient vector

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Insert

   linker
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDECKO_TFRC_C (with mCherry)

   Plasmid

   #72627

   Purpose

   Expresses two gRNAs targeting the TFRC promoter

   Depositor

   Roderic Guigo, Rory Johnson

   Article

   Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

   Insert

   gRNAs toward TFRC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 (gRNA1) and H1 (gRNA2)

   Available since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET29a-human histone 4-in-One

   Plasmid

   #223237

   Purpose

   Polycistronic bacterial coexpression construct for human His-Sumo-H2A, H2B, H3, and Dual-StrepII-H4

   Depositor

   Weixing Zhao

   Inserts

   His6-VSVG-Histone H2A
   Histone H2B
   Histone H3
   Histone H4-TEV-His6-Dual StrepII
   

   Use

   Tags

   Dual StrepII, His6, TEV protease site, and VSVG

   Expression

   Bacterial

   Mutation

   A103G

   Promoter

   T7

   Available since

   Aug. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. mEGFP-pBAD

   Plasmid

   #54622

   Purpose

   Localization: Protein Expression Vector , Excitation: 488, Emission: 507

   Depositor

   Michael Davidson

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   6xHis and mEGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. NylCK-TS_pET28a

   Plasmid

   #215423

   Purpose

   Expression construct for NylCK-TS gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   NylCK-TS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   S111G/A137L

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. LBR-mCherry

   Plasmid

   #172446

   Purpose

   Expression of nuclear envelope protein LBR with a C-terminal mCherry tag

   Depositor

   Stephen Royle

   Article

   Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

   Insert

   lamin B receptor (LBR Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGGS-flpE-puro

    Plasmid

    #20733

    Purpose

    Mammalian expression of FLPe recombinase from the highly-expressed CAGGS promoter; puro selectable marker

    Depositor

    Rudolf Jaenisch

    Article

    Beard et al Genesis. 2006 Jan . 44(1):23-8.

    Insert

    FLPe recombinase
    

    Use

    Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    March 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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