We narrowed to 12,205 results for: 112

1. pQE30-VRN1-R249E-R289E-R296E

   Plasmid

   #159532

   Purpose

   Expresses Arabidopsis thaliana VERNALIZATION1 mutant, with R249E, R289E and R296E mutations, in E. coli

   Depositor

   Joshua Mylne

   Article

   King et al J Biol Chem. 2013 Feb 1;288(5):3198-207.

   Insert

   VERNALIZATION1 (VRN1 Mustard Weed)
   

   Tags

   Six-Histidine tag

   Expression

   Bacterial

   Mutation

   Three charge reversal mutations, namely R249E R28…

   Promoter

   T5

   Available Since

   Dec. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. px335-sgRNA-EF(Cas9n)-mHdac1-L2

   Plasmid

   #122307

   Purpose

   Expresses sgRNA targeting mouse Hdac1 and Cas9 nickase in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   sgRNA for mouse Hdac1 (Hdac1 Synthetic)
   

   Expression

   Mammalian

   Available Since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-sACE2v5

   Plasmid

   #145152

   Purpose

   Mammalian expression plasmid for soluble ACE2 (protease domain) high affinity variant 5

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease domain (a.a. M1-D…

   Promoter

   CMV

   Available Since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-sACE2-T92Q

   Plasmid

   #145156

   Purpose

   Mammalian expression plasmid for soluble ACE2 (protease domain) T92Q glycosylation mutant

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease domain (a.a. M1-D…

   Promoter

   CMV

   Available Since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCEP4-BG505.SOSIP-gp160-QES.i05.c06-754*

   Plasmid

   #123275

   Purpose

   Mammalian expression plasmid for Env from the BG505 HIV-1 isolate (containing SOSIP mutations); C-terminal truncation; QES mutant for enhanced presentation of quaternary epitopes

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   HIV-1 (BG505) Env
   

   Tags

   CD5 leader peptide

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene; SOSIP mutations; …

   Promoter

   CMV

   Available Since

   April 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKP134 [pRS406-YBR139Wp-YBR139W(S219,D415,H474A)-GFP-ADH1t]

   Plasmid

   #106469

   Purpose

   Expresses Atg42/Ybr139w(S219,D415,H474A) with a C-terminal GFP tag in yeast cells

   Depositor

   Daniel Klionsky

   Article

   Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

   Insert

   YBR139W(S219,D415,H474A)
   

   Tags

   GFP

   Expression

   Bacterial and Yeast

   Mutation

   Changed Serine 219, Aspartate 415 and Histidine 4…

   Promoter

   YBR139W

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC19-Q03JI6

   Plasmid

   #103123

   Purpose

   Cas9 coding gene template with optimized sequence for human codon usage

   Depositor

   Duhee Bang

   Article

   Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

   Insert

   Q03JI6 (Cas9 coding gene from Campylobacter jejuni)
   

   Use

   CRISPR; Cloning vector

   Tags

   SV40NLS

   Mutation

   human codon-optimized

   Available Since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. TNNI3K gRNA (BRDN0001487146)

   Plasmid

   #77896

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TNNI3K

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   FPGT-TNNI3K,TNNI3K (TNNI3K, FPGT-TNNI3K Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. TNNI3K gRNA (BRDN0001146965)

   Plasmid

   #77897

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TNNI3K

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   FPGT-TNNI3K,TNNI3K (TNNI3K, FPGT-TNNI3K Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. TNNI3K gRNA (BRDN0001147407)

    Plasmid

    #77898

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TNNI3K

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    FPGT-TNNI3K,TNNI3K (FPGT-TNNI3K, TNNI3K Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. ADK gRNA (BRDN0001146463)

    Plasmid

    #75742

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ADK

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ADK (ADK Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. ADK gRNA (BRDN0001146410)

    Plasmid

    #75743

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ADK

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ADK (ADK Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. ADK gRNA (BRDN0001146622)

    Plasmid

    #75744

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ADK

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ADK (ADK Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. ADK gRNA (BRDN0001147557)

    Plasmid

    #75745

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ADK

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ADK (ADK Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. RalA (G23V)(mature peptide)-pcw107-V5

    Plasmid

    #64643

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    RALA (RALA Human)
    

    Use

    Lentiviral

    Tags

    V5

    Mutation

    G23V

    Promoter

    PGK

    Available Since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

16. RalA (G23V)(mature peptide)-pcw107

    Plasmid

    #64642

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    RALA (RALA Human)
    

    Use

    Lentiviral

    Mutation

    G23V

    Promoter

    PGK

    Available Since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pMIR-Report-SPRED1-3'UTR (mut - miR-21 site1)

    Plasmid

    #62580

    Purpose

    Translational Luciferase Reporter encoding a mutated fragment of the SPRED1 3'UTR. The upstream miR-21 (site 1) binding site was mutated.

    Depositor

    Michael Ruppert

    Article

    Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

    Insert

    SPRED1 (SPRED1 Human)
    

    Use

    Luciferase

    Mutation

    miR-21 binding site 1 mutant (TAAGCTA --> TAGA…

    Available Since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pMIR-Report-SPRED1-3'UTR (mut - miR-21 site 2)

    Plasmid

    #62581

    Purpose

    Translational Luciferase Reporter encoding a mutated fragment of the SPRED1 3'UTR. The downstream miR-21 binding site (site 2) was mutated.

    Depositor

    Michael Ruppert

    Article

    Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

    Insert

    SPRED1 (SPRED1 Human)
    

    Use

    Luciferase

    Mutation

    miR-21 binding site 2 mutant (TAAGCTA --> TAGA…

    Available Since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pLentiCRISPRv2_mSTING_gRNA_2

    Plasmid

    #196626

    Purpose

    Knock-out STING in murine cells, gRNA 2.

    Depositor

    Nicolas Manel

    Article

    Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

    Insert

    mSTING gRNA 2 (Sting1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Mutation

    WT

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV.CBA.eGFP.2A.P301L-Tau

    Plasmid

    #140425

    Purpose

    AAV.CBA.eGFP.2A.P301L-Tau propagation reporter P301L-2N4R-hTau

    Depositor

    Bradley Hyman

    Article

    Wegmann et al Sci Adv. 2019 Jun 26;5(6):eaaw6404. doi:

    Insert

    eGFP-2A-Tau(P301L) (MAPT Human, Synthetic)
    

    Use

    AAV

    Tags

    eGFP

    Mutation

    changed Proline 301 to Leucine

    Promoter

    CBA

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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