We narrowed to 25,346 results for: Spr
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Plasmid#176249PurposepNOC episomal plasmid harboring the humanized fnCas12a gene sequence without Nlux tag and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 3DepositorInserthumanized fnCas12a
UseCRISPR and Synthetic Biology; Expression in micro…Available SinceNov. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUFlip-floxed2A-KalTA4-; gcry1:BFP -2
Plasmid#173891PurposeDonor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld methodDepositorInsert2A-KalTA4; gcry1: BFP
UseSynthetic BiologyAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKLV-gRNA-Bcan-Ntrk1_4
Plasmid#136413PurposeLentiviral expression of gRNAs targeting intron 13 of murine Bcan and intron 10 of murine Ntrk1. Also constitutively expresses Puromycin linked to TagBFP.DepositorInsertU6_sgRNA(Bcan)_U6_sgRNA(Ntrk1)
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKLV-gRNA-Myb-Qk_1
Plasmid#136415PurposeLentiviral expression of gRNAs targeting intron 4 of murine Qk and intron 9 of murine Myb. Also constitutively expresses Puromycin linked to TagBFP.DepositorInsertU6_sgRNA(Myb)_U6_sgRNA(Qk)
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
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gh130
Plasmid#106800Purposeexpression of gRNA targeting CSGALNACT2DepositorInsertCSGALNACT2 (CSGALNACT2 Human)
ExpressionMammalianAvailable SinceNov. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pJSC115 - Bacterial expression plasmid for SpCas9-HF1, HNH FRET variant
Plasmid#101210PurposeBacterial expression plasmid for SpCas9-HF1, HNH FRET variantDepositorInsertSpCas9 variant C80S/C574S/S355C/S867C/N497A/R661A/Q695A/Q926A
Tags10x His, MBP, and TEV siteExpressionBacterialMutationC80S, C574S, S355C, S867C, N497A, R661A, Q695A an…PromoterT7Available SinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJSC038 - Bacterial expression plasmid for SpCas9∆REC3, HNH FRET variant
Plasmid#101203PurposeBacterial expression plasmid for SpCas9∆REC3, HNH FRET variantDepositorInsertSpCas9 variant C80S/C574S/S355C/S867C/M1–N497,GGS,V713–D1368
Tags10x His, MBP, and TEV siteExpressionBacterialMutationC80S, C574S, S355C, S867C, M1-N497, GGS, V713-D13…PromoterT7Available SinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJSC131 - Bacterial expression plasmid for SpCas9-HF1, REC3 FRET variant
Plasmid#101212PurposeBacterial expression plasmid for SpCas9-HF1, REC3 FRET variantDepositorInsertSpCas9 variant C80S/C574S/S701C/S960C/N497A/R661A/Q695A/Q926A
Tags10x His, MBP, and TEV siteExpressionBacterialMutationC80S, C574S, S701C, S960C, N497A, R661A, Q695A an…PromoterT7Available SinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only